Navigation Links
Cell Proliferation Kit Outperforms the Competition in the Range of Cells,,,Typically Counted


Quantitating cell number is more accurate using the Quantos cell proliferation assay kit

Leslie S. Arrington
Genetic Applications LLC

The Quantos cell proliferation assay kit provides a simple, reproducible method to assess the number of cells present in various tissue culture dish formats. In this study, we compared how well the kit performed compared to another commercial fluorescence-based cell proliferation assay product. The data demonstrated that the kit more accurately assessed cell number in the range of cell number frequently found in commonly used culture dish formats.

Researchers are frequently interested in determining the effects of various drugs, toxins, or experimental conditions on cell proliferation. With Stratagenes new Quantos cell proliferation assay kit, it is easy to detect cell number directly in the tissue culture well: After aspirating cell culture media, add the Quantos dye reagent, and measure fluorescence in each well using a fluorometer. Since the Quantos dye fluoresces only when bound to DNA, the assay is insensitive to changes in cellular RNA concentrations or proteins. Relative fluorescence units from the sample wells are compared to a standard curve prepared beforehand. In this way, the number of cells in each well of a tissue culture plate can be quickly and accurately determined.

Comparing Cell Proliferation Assays

We set out to determine how well the Quantos cell proliferation assay kit compares to another widely available cell proliferation assay product that uses a proprietary green fluorescent dye. Standard curves were made using adherent and suspension cells at various cell densities ranging from 0 to 80,000 cells/well in a 96-well format. The results were plotted, and trendlines for each assay were calculated.

Figure 1'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
2. Cell Proliferation and Viability Measurement
3. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
4. Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer
5. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
6. Cell Proliferation Assays
7. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
8. TripleMaster PCR SystemThe Ideal Choice for Long-Range PCR
9. Preparation of PCR Quality Template DNA from a Wide Range of Tissues Using InstaGene Matrix
10. Laser Capture Microdissection (LCM) and Expression Profiling of Long-Range Projection Neurons
11. Detection of N-in-1 Experiments: Extending the Range of N Using the API 3000 LC/MS/MS System
Post Your Comments:
(Date:8/1/2014)... , Aug. 1, 2014 AACC, a ... health through laboratory medicine, unveiled an updated brand identity ... professionals adapt to the changing healthcare environment and continue ... to physicians, so patients get the care they need. ... up AACC,s membership play an essential role in saving ...
(Date:8/1/2014)... , Aug. 1, 2014 Research and ... "Global Single Nucleotide Polymorphism (SNP) Genotyping and Analysis Market ... http://photos.prnewswire.com/prnh/20130307/600769 Single Nucleotide Polymorphism ... variations of SNPs in the genome of various species. ... SNP genotyping and analysis technology can analyze thousands of ...
(Date:8/1/2014)... SEATTLE , Aug. 1, 2014 /PRNewswire/ ... announce the launch of "Data InPress" ( ... tools for integrating multidimensional image data into ... DataViewer application and Video Injection Service. ... Photo - http://photos.prnewswire.com/prnh/20140731/132409 ...
(Date:7/31/2014)... Today BioSpace , the leading life sciences employment and ... the life sciences community in Illinois , ... , Michigan , Minnesota , ... and Wisconsin . BioMidwest is ... job postings on BioSpace originating in this region in 2013. The life ...
Breaking Biology Technology:AACC Debuts Refreshed Brand Identity 2Global Single Nucleotide Polymorphism (SNP) Genotyping and Analysis Market 2014-2018 2Glencoe Software Launches Data InPress 2Glencoe Software Launches Data InPress 3Midwest Biotech Leaders Featured On BioSpace Map 2
... China, July 7 /PRNewswire-Asia-FirstCall/ -- China Clean,Energy Inc. ... "Company"),a producer of biodiesel fuel and environmentally-friendly specialty ... Republic of China,("PRC"), today announced that business conditions ... the first quarter, driven by improved demand and,higher ...
... Lexicon Pharmaceuticals, Inc. (Nasdaq: ... developing breakthrough treatments for human disease, announced today that it ... LX1032 , the company,s oral drug candidate for managing ... LX1032 is designed to reduce serotonin production in patients ...
... The Leukemia & Lymphoma Society (LLS) and The ... initiative to develop stable and immortalized Waldenstrom,s macroglobulinemia (WM) ... researchers to test potential new treatments for the disease. ... lymphoma, characterized by B-lymphocytes in the bone marrow, and ...
Cached Biology Technology:China Clean Energy Provides Business Update 2China Clean Energy Provides Business Update 3Lexicon Initiates Phase 2 Clinical Trial of LX1032 in Patients With Carcinoid Syndrome 2Lexicon Initiates Phase 2 Clinical Trial of LX1032 in Patients With Carcinoid Syndrome 3Lexicon Initiates Phase 2 Clinical Trial of LX1032 in Patients With Carcinoid Syndrome 4The Leukemia & Lymphoma Society and The International Waldenstrom's Macroglobulinemia Foundation Partner to Develop Cell Lines to Advance Research 2The Leukemia & Lymphoma Society and The International Waldenstrom's Macroglobulinemia Foundation Partner to Develop Cell Lines to Advance Research 3
(Date:7/31/2014)... experimental vaccine protected 100 percent of animal models against ... , which causes an intestinal disease that kills approximately ... print in Infection and Immunity . , ... non-human primates against the purified toxins produced by ... spore infection, a laboratory model that mimics the human ...
(Date:7/31/2014)... how the brain processes speech, potentially increasing the ... at The University of Texas at Dallas. , ... Ear and Hearing , researchers demonstrated for the ... brain,s recognition of speech sounds. , Noise-induced hearing ... affecting an estimated 15 percent of Americans between ...
(Date:7/31/2014)... step has been made to help better identify and treat ... abnormal narrowing and enlarging of medium sized arteries in the ... other organs causing damage. In a new report appearing in ... , scientists provide evidence that that FMD may not be ... show a connection to abnormalities of bones and joints, as ...
Breaking Biology News(10 mins):C. difficile vaccine proves safe, 100 percent effective in animal models 2UT Dallas study reveals effect of loud noises on brain 2Scientists discover biochemical mechanisms contributing to fibromuscular dysplasia 2
... farming and relieve the pressure on overexploited fish stocks. ... fish escape? Researchers at the University of Gothenburg have ... are urging caution:,-Until further notice transgenic fish should be ... at the Department of Zoology, University of Gothenburg. ...
... A research report featured on the cover of the September ... http://www.fasebj.org ) describes how Australian scientists developed a new gene ... to transport their cargo into our cells. As a result ... cell,s nucleus far more efficiently than in the past, raising ...
... from natural coal deposits in the Gulf of Alaska ... the Exxon Valdez tanker catastrophe. This clearly disproves the ... observed environmental damage. PAH pollutants were blamed for the ... Alaska. Then a dispute erupted over the origins of ...
Cached Biology News:Risks involved with transgenic fish 2Finding the ZIP-code for gene therapy: Scientists imitate viruses to deliver therapeutic genes 2Bioavailable contaminants come from the Exxon Valdez oil catastrophe 2
Interact with both DNA and RNA; fluorescence green (Em=525 nm) bound to DNA; and fluorescence red (Em=~650 nm) bound to RNA....
One bottle containing 30 ml of protein solution and 0.1% sodium azide....
...
Sheep polyclonal to Quinolone ( Abpromise for all tested applications). Antigen: Norfloxacin(N)conjugated to BSA...
Biology Products: