Navigation Links
Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer

William C Heiser, PhD and Ma Theresa Redila-Flores, Bio-Rad Laboratories, Inc . , Hercules, CA 94547 USA


Introduction
An accurate and rapid assessment of viable cell number and proliferation is often required for in vitro and in vivo experiments. These assessments are necessary and useful for analysis of growth factor activity, drug screening, serum batch testing, and toxicological determination of carcinogenic compounds, to name a few. Cell proliferation kits are used to measure cell viability and division in response to growth factors, cytokines, and nutrients, as well as growth inhibition by cytotoxic agents. The cell proliferation kit described here utilizes a microplate spectrophotometer to quantitate an assay based on XTT, a tetrazolium salt that is cleaved as a result of metabolic activity to generate a colored formazan product.


Method
A cell proliferation kit from Roche was used to measure proliferation of A549 cells grown at different densities overnight. The cells were incubated with XTT before measuring the absorbance. To confirm the optimal wavelength at which to read the samples, a spectral scan was done. The cells were then read after 1, 2, and 3 hr of incubation as suggested in the kit instructions.

Procedure
1. Inoculate A549 cells into a 25 cm2 flask with DMEM or appropriate medium. Grow cells to 70% confluence.

2. Wash cells with 1x PBS. Trypsinize, then add 2 ml of appropriate medium.

3. Dilute cells 10-fold, to about 2.5 x 105 cells/ml. Prepare 2-fold dilutions and plate 100 l of each dilution in replicates of 5 in a 96-well plate. Prepare a blank by dispensing
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Cell Proliferation Kit Outperforms the Competition in the Range of Cells Typically Counted
2. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
3. Cell Proliferation and Viability Measurement
4. measuring cell proliferation with SpectraMax M5, M2 and Gemini microplate readers using the CyQUANT Cell Proliferation Assay
5. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
6. Cell Proliferation Assays
7. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
8. Luminol-enhanced Assay for Superoxide Anion (O2 face="Symbol" )
9. Organize Your Data from the Big Blue Mutation Assay
10. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
11. RNase Protection Assay (RPA) Using DIG-Labeled RNA Probes
Post Your Comments:
(Date:8/18/2014)... - SQI Diagnostics Inc. (TSX-V: SQD), a life sciences company ... advanced microarray diagnostics, today announced it will hold an ... pm ET.  During the call, ... of SQI,s recent commercialization progress and then introduce Dr. ... appointed to the Board of Directors of SQI and ...
(Date:8/18/2014)... YORK , August 18, 2014 ... published by Transparency Market Research "Life Science Reagents ... Flow Cytometry, Electrophoresis, Chromatography, In-Vitro Diagnostic Reagents and ... and Academic Institutions, Clinical Laboratories, and Forensic Laboratories)- ... Forecast, 2013 - 2019", the global life science ...
(Date:8/18/2014)... 18. August 2014 Vier führende ... internationale klinische Studie auf, in der eine ... soll das Sehvermögen bei allen Entfernungen verbessern, ... möglicherweise keine Lese- oder Gleitsichtbrille mehr benötigen. ... FluidVision Accommodating Intraocular Lens (FluidVision ...
(Date:8/15/2014)... FOREST, Ill. , Aug. 15, 2014   ... world,s leading provider of injectable drugs and infusion technologies, ... Morgan Stanley Global Healthcare Conference on Tuesday, Sept. 9, ... The presentation is scheduled to begin at 11:45 ... will be available to all interested parties through a ...
Breaking Biology Technology:SQI Diagnostics Announces Investor Conference Call with Dr. Allan Pronovost 2SQI Diagnostics Announces Investor Conference Call with Dr. Allan Pronovost 3Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 2Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 3Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 4Global Life Science Reagents Market Expected to Reach USD 25.7 Billion in 2019: Transparency Market Research 5Implantation einer revolutionären akkommodativen Linse bei Katarakt-Patienten in deutschen Augenzentren im Rahmen einer internationalen klinischen Studie 2Implantation einer revolutionären akkommodativen Linse bei Katarakt-Patienten in deutschen Augenzentren im Rahmen einer internationalen klinischen Studie 3Implantation einer revolutionären akkommodativen Linse bei Katarakt-Patienten in deutschen Augenzentren im Rahmen einer internationalen klinischen Studie 4
... when theoretical work is eventually supported by experimentation. Such ... Italian and US scientists when they received preliminary confirmation ... that is currently orbiting the Earth on the Foton-M3 ... week ago, the scientists are already very excited about ...
... Board Reflects Emphasis on Drug Development and Recent ... ... Lexicon,Pharmaceuticals, Inc. (Nasdaq: LXRX ) announced several changes to ... drug development and the completion of a major strategic financing,with ...
... NanoBio(R) Corporation, a,biopharmaceutical company developing novel anti-infective ... infections, today announced,the addition of four new scientists ... PharmD, Ph.D.; Susan Ciotti, Ph.D.; Robert L. Hagan, ... developing and commercializing,its novel topical anti-infectives and mucosal ...
Cached Biology Technology:Fluid theory confirmed by Foton 2Lexicon Appoints Judith Swain, M.D. to Board of Directors 2Lexicon Appoints Judith Swain, M.D. to Board of Directors 3Lexicon Appoints Judith Swain, M.D. to Board of Directors 4NanoBio Expands Drug Development Team for Its Anti-Infective Products 2NanoBio Expands Drug Development Team for Its Anti-Infective Products 3
(Date:8/19/2014)... Aug. 19, 2014 To improve students, chances ... grades, the University of Houston (UH) implemented a ... students at risk for failure. , Through ... Board (THECB), the Department of Biology and Biochemistry ... students taking "General Biology" for non-majors and "Introductory ...
(Date:8/19/2014)... is available in German . ... intermittent energy sources. If a large amount of electricity is ... excess energy will have to be stored during productive periods ... storage capacities are far from adequate for the purpose. Science ... important focus lies on battery systems that used to be ...
(Date:8/19/2014)... lemur. It,s not an African Bush Baby or even a ... downright "cool" primate from Southeast Asia. , "It,s really not ... Brown, curator-in-charge at the University of Kansas, Biodiversity Institute. "A ... body; a long tail with a furry tuft at the ... a bit like the disks on the digits of tree ...
Breaking Biology News(10 mins):University of Houson program earns kudos for improving grades, retaining students 2University of Houson program earns kudos for improving grades, retaining students 3Asian inventions dominate energy storage systems 2Asian inventions dominate energy storage systems 3Philippine tarsier gets boost from Kansas research, and genetic proof of a new variety 2Philippine tarsier gets boost from Kansas research, and genetic proof of a new variety 3
... by global warming can prove extremely destructive to island habitats, ... C. Bellard, C. Leclerc and F. Courchamp of the ... possible scenarios, from optimistic to very pessimistic, to bring attention ... biodiversity hotspots worldwide. The study was published in the open ...
... measures carbon dioxide and suggests that far from being a ... a biological signalling molecule. Researchers led by Professor Nick ... of Warwick have shown that the body senses carbon dioxide ... receptor for the gas. Connexin 26 is better known as ...
... largest lake in Britain and Ireland, Lough Neagh, has lost ... to researchers at Queen,s University Belfast., The study by Quercus, ... number of diving ducks migrating to the lake for the ... in the space of a decade., The research, published in ...
Cached Biology News:Island biodiversity in danger of total submersion with climate change 2Carbon dioxide's new-found signalling role could be applied to blood flow, birth and deafness 2Largest lake in Britain and Ireland has lost three-quarters of winter water birds 2
5-bromouridine 5'-triphosphate (BrUTP) *10 mM in TE buffer*...
...
in vitro Translation, Accessory Products...
... The GenoStat Inducible Expression System provides tightly ... mammalian cells and is capable of repeated ... is mediated by the highly specific interaction ... bipartite nuclear receptor complex. Gene expression levels ...
Biology Products: