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CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

sequencing samples).

For genetic analysis, STRP banding patterns were obtained using a precast gel on the automated infrared DNA analyzer. In Figure 4, four STRP loci are detected in each lane (four multiplexed reactions per channel). Expect 1,440 genotypes (60 lanes x 4 loci x 2 channels x 3 loads) for a typical throughput per precast gel, which is equivalent to manually poured gels. Additionally, the precast gel can be reloaded three times (Figure 4), making fluorescence STRP analysis cost effective as well.

Figure 4

Conclusions

Use Stratagenes CastAway gels for the LI-COR system on the IR2 DNA Analysis System for automated DNA sequencing and genotyping applications. CastAway precast gels are made with soda-lime float glass plates, which produce minimal background fluorescence with the systems infrared technology. Therefore, it is unlikely these precast gels could be used with visible fluorescence automated DNA sequencers, such as the ABI PRISM 377, since the system sensitivity would be lower due to significant emission of background fluorescence by the soda-lime plates. Further, CastAway gels compare favorably to the standard LI-COR manually poured 25-cm gelwith respect to electrophoresis run time, band morphology, band resolution, and signal strengthand both gel types produce a similar sequence read length and accuracy.

REFERENCES
  1. Smith, L.M., et al. (1986) Nature 321: 674-679.

  2. Ansorge, W., et al. (1986) J. Biochem. Biophys. Methods 13: 315-323.

  3. Middendorf, L.R., et al. (1992) Electrophoresis 13: 487-494.

  4. Ziegle, J.S., et al. (1992). Genomics 14: 1026-1031.

  5. Gi
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