Navigation Links
CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

s: assemble the gel apparatus, mix reagents, degas the gel solution, pour the gel, and complete gel polymerization. In contrast, Stratagenes CastAway gels are provided polymerized and ready to load in less than 30 minutes (Figure 1).

The gel apparatus and precast gel are easily assembled in four steps (Figure 1). First, remove the CastAway gel from its packaging and allow it to equilibrate to room temperature. Second, rinse the front and back glass plates with distilled water and dry with lint-free wipes. Third, tighten the left and right precast gel rails and the upper buffer tank into position, and the gel assembly is complete. Finally, install the gel apparatus on the LI-COR instrument and carry out a 20 minute pre-electrophoresis prior to loading the gel.

Focusing and Electrophoresis

The LI-COR IR2 Automated DNA Analyzer uses a scanning infrared fluorescence microscope to detect the electrophoretic movement of fluorescently labeled DNA molecules in real time. The system is a two-dye infrared automated DNA sequencer that can detect the products of two sequencing reactions in parallel by generating two separate gel images, each corresponding to the fluorescence emission of a different infrared dye (700 nm channel for IRD 700; 800 nm channel for IRD 800).

The microscope consists of one or two laser diodes for providing dye excitation (wavelength at 685 nm and 785 nm) and one or two avalanche photodiodes for detecting the fluorescence emission.6,7 By using infrared radiation, the background fluorescence caused by the soda lime glass of the precast gel is greatly reduced. Moreover, variations in precast gel thickness can be accommodated by autofocusing the fluorescence microscope to
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Four New CastAway Precast Gels
2. Identification of Differentially Expressed Gene Products with the CastAway System*
3. Advantages of ReadyAgarose Precast Gels Over 96-Well Handcast Gels for High-Throughput Analysis, Rev A
4. Simple, Sensitive, and Rapid Detection of FLAG -Tagged Fusion Proteins
5. A Simple and Rapid Technique to Purify High-Quality Midiprep DNA
6. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
7. HSVision Molecular Beacon Detection Module Rapidly Detects Herpes Simplex Virus DNA
8. Simple and Rapid Isolation of Plant RNA Using the Eppendorf Perfect RNA, Eukaryotic, Maxi Kit
9. Rapid PCR* Using Active Tube Temperature Control On Thermos PCR Machines
10. Molecular Biology:Easy Dephosphorylation and Rapid DNA Ligation
11. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: CastAway Precast Gels for Rapid Automated DNA Sequence Analysis

(Date:7/26/2014)... Canada Endoscopy Visualization Systems Market Outlook ... Canada Endoscopy Visualization Systems market. The report provides ... units) and average prices (in US dollars) within ... Endoscopy Visualization Systems and Low-End Endoscopy Visualization Systems. ... , The report also provides company shares and ...
(Date:7/25/2014)... bacteria live in each person,s digestive tract. Scientists believe ... stave off harmful infections, but their role in human ... light on the role of these bacteria, a team ... recently tracked fluctuations in the bacterial populations of two ... in the July 25 issue of the journal ...
(Date:7/25/2014)... New York (PRWEB) July 25, 2014 ... Market Research, "Biological Drugs Market - Global Industry Analysis, ... the global biological drugs market is estimated at USD ... at a CAGR of 10.1% from 2014 to 2020, ... in 2020. , The biological drugs market is witnessing ...
(Date:7/25/2014)... Livermore, California (PRWEB) July 25, 2014 ... been named to Thomson Reuters list of " The ... Pitz are part of the 3,000 researchers who were ... years to recognize those who published the highest-impact work ... numerous research papers on combustion modeling . , ...
Breaking Biology Technology:Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5Monitoring the rise and fall of the microbiome 2Monitoring the rise and fall of the microbiome 3Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3Scientists Burst onto Influential List for Combustion Research 2Scientists Burst onto Influential List for Combustion Research 3Scientists Burst onto Influential List for Combustion Research 4
... is a missing link in the capital food chain, and Bill ... devoured by it. , ,Payne, an entrepreneur-in-residence for the Kauffman ... to address the June 25 Wisconsin Alumni Research Foundation's ... the capital food chain for entrepreneurs, and he's familiar with all ...
... have pleaded guilty in Milwaukee to charges of criminal ... $6 million in counterfeit Rockwell Automation computer ... Alice Fisher for the Criminal Division and U.S. Attorney ... announced that Robert Koster of Jonesboro, Ark. pleaded ...
... print still captures the imagination of consumers - even consumers ... clearer example than Blurb . I first discussed Blurb ... than a brief PowerPoint deck. We met at ... unwound a vision for consumer-generated books that well pre-dates the ...
Cached Biology Technology:Investor warns of angel-venture funding gap 2Investor warns of angel-venture funding gap 3Two convicted of selling $6 million in counterfeit Rockwell software on eBay 2Blurb shows that print still captures imaginations 2Blurb shows that print still captures imaginations 3
(Date:7/25/2014)... factor characterized by the existence of numerous isoforms ... to the protein deeply different characteristics. NRG1 plays ... during development and the different phases occurring after ... regrowth, remyelination and target reinnervation, Researchers at the ... soluble NRG1 upregulation observed in Schwann cells immediately ...
(Date:7/25/2014)... tractography (DTT), which is derived from diffusion tensor ... motor tracts, such as the corticospinal tract, the ... The corticospinal tract is known to be a ... human brain. Several studies have reported on injury ... addition, some studies have demonstrated recovery of a ...
(Date:7/25/2014)... MOResearchers at the Stowers Institute for Medical Research ... pheromone receptors, and found pheromones crucial to triggering the ... of receptors helps a male mouse detect pheromones that ... of receptors lets him know if the female mouse ... pheromones are critical to trigger mating. Stowers, researchers believe ...
Breaking Biology News(10 mins):It takes two to court 2It takes two to court 3
... technologies are repairing the human body in ways never ... leaving an increasing population of patients who are newly ... have become a formidable clinical opponent, and foremost among ... a new text from ASM Press. "This ...
... This Christmas season, think twice about spending money on ... good, old-fashioned methodkeeping your tree in a container of ... tree green and healthy. Researchers have determined that some ... fact, in several cases the chemical retardants sped up ...
... In light of recent developments that threaten to lead to ... daily satellite images of the ice shelf available to the ... to monitor the developments as they occur. The Wilkins ... coast of the Antarctic Peninsula, has undergone many changes in ...
Cached Biology News:New text focuses on formidable opportunistic fungus 2Flame retardants prove ineffective on fresh-cut Christmas trees 2
Mouse monoclonal antibody raised against a partial recombinant AATK. NCBI Entrez Gene ID = AATK...
... The Rotofor purification system with PowerPac 3000 ... protein separation and purification by liquid-phase isoelectric ... mini (18 ml) and standard (60 ml) ... electrode chambers, PowerPac 3000 power supply, 10 ...
... Bio-Rad Molecular Imager VersaDoc MP 4000 ... CCD based imaging system capable of ... methods, including fluorescence, chemiluminescence, densitometry, and ... illumination sources allow imaging of multiplexed ...
...
Biology Products: