Navigation Links
Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays

Introduction

The understanding of the cellular signaling processes leading to programmed cell death (apoptosis) is of utmost importance in the study of autoimmune diseases such as rheumatoid arthritis or Parkinsons and Alzheimers disease. Here, errors in the signaling cascade are believed to lead to premature apoptosis in the affected tissue. At the same time, damaged cells that do not enter the apoptotic pathway may proliferate unchecked and become a cancerous cell mass. Thus, the study of apoptosis benefits many different fields of medicine by offering multiple targets for specific drug treatment.

Caspases (cysteinyl aspartate-specific proteases) are a family of important signaling molecules with various tasks depending on subtype and organ. The activation of caspases is also a marker for cellular damage in diseases such as stroke or myocardial infarction (reviewed by Lavrik et al . 2005). Although the precise role in the initiation and progression of apoptosis is not known for all caspases, their involvement as an indicator alone and as a potential leverage point for drug treatment make them widely researched molecules.

We explored the use of the active form of caspase-3 for the detection of apoptotic events. This protease has been implicated as an effector caspase associated with the initiation of the death cascade and is therefore an important marker of the cells entry point into the apoptotic signaling pathway (Nicholson et al . 1995). Caspase-3 is activated by the upstream caspase-8 and caspase-9 and since it serves as a convergence point for different signaling pathways, it is well suited as a read-out in an apoptosis assay. Detection of active caspase-3 can be used in different cell lines or primary cells, does not require the use of transfection techniques and can be multiplexed with other probes to get an in-depth understanding of signaling events with cell-by-cel
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
2. Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
3. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
4. Monitoring Receptor-Mediated Changes in [Ca2+]i Using Single-Wavelength and Dual-Wavelength Indicators on the FlexStation Workstation
5. RT-PCR Primers for the Study of Apoptosis
6. A Further Step in Understanding Apoptosis Direct Detection of PARP Cleavage
7. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
8. Promegas Multiplexed Cell Viability and Apoptosis Assays performed on the PHERAstar
9. An Image-Based Assay of Endothelial Cell Tube Formation as a Model of Angiogenesis
10. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
11. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Caspase Activation Indicator Apoptosis Image Based Assays

(Date:9/22/2014)... Synageva BioPharma Corp. ("Synageva") ... developing therapeutic products for rare disorders announced today ... Chief Operating Officer.  Reporting directly to Sanj ... Robert will oversee the planned global commercial launch ... LAL Deficiency, and will have responsibility for the ...
(Date:9/21/2014)... to produce ultra-thin "diamond nanothreads" that promise extraordinary properties, ... strongest nanotubes and polymers. A paper describing this discovery ... a professor of chemistry at Penn State University, will ... the journal Nature Materials . , "From a ... the threads we formed have a structure that has ...
(Date:9/20/2014)... (PRWEB) September 20, 2014 ... urological and interventional radiology products and services, has ... subsidiary with Clinicon Corporation for its ... HealthTronics’ portfolio of minimally invasive, mobile, surgical technology—offering ... , Under the agreement, HealthTronics will make available ...
(Date:9/19/2014)... disease risks, the biological characteristics people care most about ... heightthe textbook example of a complex traitattributes like risk ... genetic and environmental influences, making it challenging to find ... typically mate two individuals that differ in key waysfor ... study their descendents, looking for genes that tend to ...
Breaking Biology Technology:Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 2Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 3Synageva BioPharma Appoints Robert Bazemore As Chief Operating Officer 4Smallest possible diamonds form ultra-thin nanothreads 2Smallest possible diamonds form ultra-thin nanothreads 3Smallest possible diamonds form ultra-thin nanothreads 4HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 2HealthTronics Announces Distribution Agreement with Clinicon-Laser Surgical Device Expands Minimally Invasive, Mobile Technology Portfolio 3Want to link genes to complex traits? Start with more diversity 2
... IgG1 Using UNOsphere S and CHT , ... , Henry Lai, PhD, and Samuel G Franklin, PhD, ... USA , , ... a variety of sources have been purified , using ...
... Agencourt offers primer walking sequencing of PCR products, ... submit your template in either raw or , ... Select from either single stranded coverage for confirmation sequencing , ... clone validation , purposes. , ...
... , RNAClean is a ... isolates RNA or cDNA from in vitro transcription, cDNA or RNA ... As gene expression profiling , analysis and ... effective, automated purification reagent is required. Traditional purification , ...
Cached Biology Technology:Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 2Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 3Purification of Murine IgG1 Using UNOsphere S and CHT , Ceramic Hydroxyapatite Chromatography, Rev A 4Primer Walking 2RNA & cDNA Purification Using RNAClean 2RNA & cDNA Purification Using RNAClean 3RNA & cDNA Purification Using RNAClean 4RNA & cDNA Purification Using RNAClean 5
(Date:9/19/2014)... will study multifunctional and specialized spinal cord nerve ... Science Foundation grant in the amount of $680,000 ... in the Department of Biology, OU College of ... between the multifunctional and specialized spinal cord nerve ... what connections they make. Berkowitz will collaborate ...
(Date:9/19/2014)... a species which over thousands of years has adapted to ... substance that makes the cold dangerous for you. , This ... , and the cold provoking substance, called nonylphenol, comes from ... is suspected of being a endocrine disruptor, but when entering ... worm,s ability to protect the cells in its body from ...
(Date:9/19/2014)... A new study shows that legume plants regulate ... cytokininssignaling molecules that are transmitted through the plant ... the number of bacteria-holding nodules in the roots. ... the National Institute for Basic Biology, the Graduate ... Center for Sustainable Resource Science in Japan. ...
Breaking Biology News(10 mins):Environmental pollutants make worms susceptible to cold 2Environmental pollutants make worms susceptible to cold 3For legume plants, a new route from shoot to root 2
... has demonstrated how a technique using lasers could be ... campaign supports one of the main objectives of the ... Carbon and climate Observation of Planet Earth) is one ... just completed assessment study. The mission concept, along with ...
... International Society for Stem Cell Research (ISSCR), the world,s ... guidelines for the responsible development of safe and effective ... summarizes the Guidelines for the Clinical Translation of Stem ... December issue of Cell Stem Cell , the ...
... for pregnancy - other asthma drugs have not been as ... using the same asthma drugs they were using before they ... be regarded as safe. More trials of other essential medicines ... and Efficiency in Health Care (IQWiG), more data from trials ...
Cached Biology News:ESA tests laser to measure atmospheric carbon dioxide 2ESA tests laser to measure atmospheric carbon dioxide 3The International Society for Stem Cell Research releases new guidelines 2The International Society for Stem Cell Research releases new guidelines 3Pregnant women with asthma can be more confident about some medicines 2
Unit Definition: 1 U corresponds to the amount of enzyme which produces 1 mol protons per minute at pH 5.5 and 30C (peptin from citrus peel, Fluka-No. 76280, as substrate)...
ANTI STENOTROPHOMONAS MALTOPHILIA...
... tube handling and reagent dispensing Holds: ... - 96 x 0.3ml U-bottom Omnistrips ... Non-Skirted Plate - Thermo-Fast® 96 Semi-Skirted Plate ... Multiple workstations can be securely stacked Can be stored ...
... well Magnetic-Ring Stand is designed for paramagnetic ... microplates. The stand has a plastic base ... plate footprint. The use of 96 extremely ... easy magnetic separation. With this magnetic stand, ...
Biology Products: