Navigation Links
Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform

es and subsequently lead to oncogenesis.


Figure 4. Cancer-related miRNAs and Their Putative Oncogene Targets.


Measuring the Effect of miRNAs on Oncogene Expression

Confirming miRNA target site predictions can be done in a variety of ways. In Drosophila and C. elegans, genetic approaches have been applied wherein mutations in the miRNA and the putative miRNA target site(s) are made and shown to result in similar phenotypes [8, 9]. In mammalian cells, where genetic approaches are far more difficult, reporter constructs have been used to show that the 3' UTRs of putative target genes are regulated in cells at levels that are disproportionate to reporter vector controls that contain mutations in the putative miRNA binding sites [10]. In addition, vectors and oligonucleotides have been used to introduce or inhibit miRNAs in cells to determine the effects on endogenous levels of putative target genes [10, 11]. We have taken the latter approach to validate our miRNA target site predictions.

Ambion recently introduced a series of precursor miRNAs (Pre-miR miRNA Precursor Molecules, Cat# 17100) and miRNA inhibitors (Anti-miR miRNA Inhibitors, Cat# 17000) that can be transfected into mammalian cells to either introduce miRNAs into cells or inhibit the activity of miRNAs in cells, respectiv
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Precursor miRNAs for Successful miRNA Functional Studies
2. Detecting miRNA & siRNA
3. miRNA Expression in White Blood Cells
4. Isolate It All: siRNA miRNA Total RNA Native Protein
5. Get the miRNA OutPlant Applications
6. Novel MicroRNA Array Technology for Sensitive miRNA Profiling
7. pSilencer 4.1-CMV: Versatile Vectors for Expression of siRNA, miRNA, and mRNA
8. Prepare siRNA and miRNA Probes in Just 1 Hour
9. Reagents for Up and Down-Regulation of miRNA Activity in Mammalian Cells
10. New Microarray Labeling Kit for Preparing cDNA Probes
11. Stratagenes Human cDNA Microarrays: Perform Gene Expression Profiling and Discover New Genes
Post Your Comments:
(Date:10/22/2014)...   Synthetic Biologics, Inc. (NYSE MKT: SYN), ... infections and diseases, announced today that the U.S. Patent ... for a composition of matter patent application that covers ... SYN-004. This is Synthetic Biologics, first allowed patent application ... to the Company,s extensive C. difficile patent ...
(Date:10/22/2014)... KONG , Oct. 22, 2014  aTyr Pharma ... that rare disease expert John C. McKew , ... McKew brings more than two decades of expertise in ... Institutes of Health, Wyeth Research and Genetics Institute, Inc. ... aTyr,s efforts to expand and translate its novel Physiocrine ...
(Date:10/20/2014)... 2014 Asterias Biotherapeutics, Inc. (NYSE MKT: ... a Notice of Grant Award (NGA) with the ... 1, 2014.  The NGA provides for the immediate ... of additional grant funds pursuant to the previously ... development of Asterias, product, AST-OPC1. The grant provides ...
(Date:10/20/2014)... Oct. 20, 2014 PureTech , a ... big healthcare problems, announced today the closing of ... participation from Invesco Perpetual, a $120 Billion group ... drive PureTech,s existing pipeline forward and to advance ... the scientific creativity to really go for the ...
Breaking Biology Technology:Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 2Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 3Synthetic Biologics Announces Allowance of Key U.S. Composition of Matter Patent for C. difficile Program 4aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 2aTyr Pharma Appoints John C. McKew, Ph.D., as Vice President, Research 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6
... 19 , - Shocking Lack of Global Statistics ... Atlas, The World Hepatitis Alliance, a newly formed ... do more to,drive improvements in prevention, diagnosis and treatment ... Although it is estimated that 500 million, approximately ...
... Biosystems,Inc. (OTC Bulletin Board: WGBS), a leading developer of state-of-the-art,gene ... the signing of two separate private placements. The details,of the ... -- WaferGen has agreed to sell shares of the ... certain funds for which Brencourt Advisors is the ...
... YORK, May 19 Intellect Neurosciences,Inc. (OTC ... on,development of disease-modifying therapeutic agents for the ... today that it has entered,into a license ... and Elan Pharma,International Ltd. ("Elan") regarding certain ...
Cached Biology Technology:World Hepatitis Alliance Calls on Governments to Take Urgent Action to Tackle Chronic Viral Hepatitis B & C Epidemic 2World Hepatitis Alliance Calls on Governments to Take Urgent Action to Tackle Chronic Viral Hepatitis B & C Epidemic 3WaferGen Announces Signing of Two Private Placements 2WaferGen Announces Signing of Two Private Placements 3WaferGen Announces Signing of Two Private Placements 4WaferGen Announces Signing of Two Private Placements 5Intellect Neurosciences, Inc. Grants License for Certain Patents and Patent Applications to Wyeth and Elan Pharma International Ltd. 2Intellect Neurosciences, Inc. Grants License for Certain Patents and Patent Applications to Wyeth and Elan Pharma International Ltd. 3
(Date:10/15/2014)... from strains of influenza virus increases with the latest ... complacent that the most substantial threats have been identified, ... arise when a new virus strain – against which ... in the human population. There have been five such ... which – the 1918 Spanish Flu – cost 50 ...
(Date:10/15/2014)... virus is spreading rapidly and to an unexpected extent. ... the past and the virus shows a new disease ... before. For this reason, the German National Academy of ... and Engineering, and the Union of the German Academies ... the Ebola epidemic today. , In the statement the ...
(Date:10/15/2014)... Geneticist Fred Alt will be awarded the 44th Rosenstiel ... University for his pioneering research exploring the mechanisms of ... and cancer cells. Alt is the second alumnus to ... the Nobel Prize in 2003. , Alt is the ... Genetics at Harvard Medical School and an investigator at ...
Breaking Biology News(10 mins):Scientists 'must not become complacent' when assessing pandemic threat from flu viruses 2Academies call for consequences from the Ebola virus epidemic 2Academies call for consequences from the Ebola virus epidemic 3Academies call for consequences from the Ebola virus epidemic 4Academies call for consequences from the Ebola virus epidemic 5Brandeis awards 44th Rosenstiel Award to pioneering geneticist Fred Alt 2
... today a $480,000 gift from the Emily Landecker Foundation. ... in Astrobiology, which will support graduate students in the ... of astrobiology. The fellowship is named in honor of ... currently a senior research professor, and a member of ...
... yuck factor, have captured the hearts of two University of ... this week is publishing the leech,s complete genome sequence. ... UC Berkeley professor of molecular and cell biology who lobbied ... that has targeted a variety of animals in order to ...
... Medical Center researchers have pinpointed a molecular mechanism needed ... step toward developing eventual therapies for damage suffered following ... UT Southwestern, teaming up to study in mice how ... that regulate gene expression contribute to the heart,s ...
Cached Biology News:Rensselaer awarded gift to establish fellowship in astrobiology 2What do leeches, limpets and worms have in common? Now, a sequenced genome 2What do leeches, limpets and worms have in common? Now, a sequenced genome 3Research pinpoints key gene for regenerating cells after heart attack 2
This buffer is Specifically formulated to be used with Endogen's Poly-HRP Streptavidin...
... Microarray Gene Frame® has been specifically ... frames feature the same dual strength ... Frame® range for easy removal after ... and there are three sizes available. ...
For microscopy...
... slide processing offering reproducibility, reliability and ... miniaturization, automation and massive parallel analysis ... with many applications in both industrial ... from gene expression profiling, gene regulation ...
Biology Products: