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Cancer-Related miRNAs Uncovered by the mirVana miRNA Microarray Platform

es and subsequently lead to oncogenesis.


Figure 4. Cancer-related miRNAs and Their Putative Oncogene Targets.


Measuring the Effect of miRNAs on Oncogene Expression

Confirming miRNA target site predictions can be done in a variety of ways. In Drosophila and C. elegans, genetic approaches have been applied wherein mutations in the miRNA and the putative miRNA target site(s) are made and shown to result in similar phenotypes [8, 9]. In mammalian cells, where genetic approaches are far more difficult, reporter constructs have been used to show that the 3' UTRs of putative target genes are regulated in cells at levels that are disproportionate to reporter vector controls that contain mutations in the putative miRNA binding sites [10]. In addition, vectors and oligonucleotides have been used to introduce or inhibit miRNAs in cells to determine the effects on endogenous levels of putative target genes [10, 11]. We have taken the latter approach to validate our miRNA target site predictions.

Ambion recently introduced a series of precursor miRNAs (Pre-miR miRNA Precursor Molecules, Cat# 17100) and miRNA inhibitors (Anti-miR miRNA Inhibitors, Cat# 17000) that can be transfected into mammalian cells to either introduce miRNAs into cells or inhibit the activity of miRNAs in cells, respectiv
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