miRNA Expression in Lung Cancer
Twenty-two tumor and normal adjacent tissue (NAT) samples from lung cancer patients were analyzed using mirVana miRNA array platform. The arrays were analyzed, and the relative expression of each miRNA was compared between the tumor and normal adjacent tissues from each patient. The various miRNAs were clustered based on their relative expression in tumors across different patients (Figure 1A). Six miRNAs (miR-126, -30a, -143, -145, -188, and -331) were expressed at significantly lower levels in the tumors of more than 70% of the patients. Three miRNAs (miR-21, -189, and -200b) were expressed at significantly higher levels in the tumors of more than 70% of the patients. The differential expression of a number of these miRNAs was verified by Northern analysis (Figure 2).
Figure 2. Validation
of miRNA Array Expression Results in Lung Cancer Patients.
Total RNA samples from two lung cancer patients were analyzed for expression
of miR-16, miR-21, miR-143, miR-145, and let-7 using Northern analysis.
The graphs show the relative abundance of each miRNA (ratio of tumor:NAT)
from the array analysis and Northern phosp