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Calcium Plus Assay Kit, Superior Detection for Specialized Targets


HOMOGENEOUS ASSAY

HIGHER MAXIMUM SIGNAL

RAPID PROCEDURE

COMPATIBLE WITH MORE TARGETS

SUITABLE FOR HTS APPLICATONS


The Calcium Plus Assay Kit provides a reliable and homogeneous method for detecting changes in intracellular calcium concentration. Specifically, this fast and simple fluorescencebased assay allows the researcher to accurately and easily detect intracellular calcium fluxes in targets that generate unreliable data using some traditional methods. Further, the Calcium Plus Assay Kit is designed for use with chemokine and small peptide targets, which can be problematic and produce less than optimal results with standard assay formats.


IMPROVED RESULTS
The primary advantage of the Calcium Plus Assay Kit is the improvement in the data quality. When compared with the standard fluo-3 AM dye incubation and wash procedure, results using the Calcium Plus Assay Kit are more consistent. Advances in the reagent chemistry and control of addition volumes are the pivotal reasons for the enhanced performance. These developments result in fewer spontaneous calcium fluxes and unresponsive cells. Figure 1 illustrates dose:response traces generated using the FLIPR384 system. Note the signal dynamic range and the small addition volume (6 L).

Strong signals are attained in cases that may previously have yielded no peak at all. When compared against assays using fluo-3, the peaks generated with the Calcium Plus Assay Kit are substantially higher and more distinct. Additionally, tighter CVs across 96- and 384- well plates are observed. Figure 2 illustrates the difference in kinetics and dynamic range between the equivalent concentrations of RANTES and MIP1a. '"/>

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