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Calcium Flux Assays using the LabChip 3000

ity over multiple runs. Figure 8 shows the calculated Z' values for the agonist assay with both a 12 and 5 s sample sip time. Assay stability was measured over several runs and was determined to be adequate over the intended run time of between 4 and 8 hr. Average Z' values were calculated at 0.86 and 0.75 over 8 hr for the 12 and 5 s sample sip times, respectively.

Figure 9 shows the Z' values for the associated antagonist assay, with stability and reproducibility within expected limits. Average Z' values were calculated at 0.72 and 0.57 over 5 hr for the 12 and 5 s sample sip times, respectively. Note that the shorter sip time does result in slightly lower Z' values, as discussed in section 8.

In addition to Z' analysis , the stability of both the agonist and antagonist response was measured over several runs for both 12 and 5 s sample sip times. Figures 10 and 11 show the stability of the agonist and antagonist response relative to an ionomycin and atropine control, respectively, over several hours for a 12 s sample sip time. The 5 s sample sip response is similar over an extended run (data not shown).


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