Navigation Links
Calcium Flux Assays using the LabChip 3000

ty of the assay can be assessed by examining the cell count rate and the cell viability (e.g. EC50) over time.

CHO-m1 cells are loaded in the chip wells at a density of between 3-5 x 106 cells/mL. The cell count over time is monitored to ensure that sufficient statistics are gathered on the last plate of the run. Figure 5 shows that cell counts are > 100 cells per well after 5 hr. Figure 6 shows the stability of the cell response over a typical run time.

8. Optimize Throughput and Cell Usage
Optimization of throughput is achieved by gathering data on a sufficient number of cells for each sample well visited on all assay plates. Figure 7 shows the dependence of data quality (Z') on cell count per sample. Typically more than 50 cells per sample are required for sufficient statistics to develop high Z' assays.

As cell count numbers are dependent on sample sip time, throughput and data quality can be optimized as needed. In this assay, sample sip times of 12 and 5 s were chosen as low- and high-throughput versions of both agonist and antagonist calcium flux assays. The sip time of degassed buffer between samples should also be chosen to maximize throughput while still maintaining a low dissolved gas content on the chip. Buffer sip times of 5 and 3 s were chosen for 12 and 5 s sample sip times, respectively.

The number of wells sampled during an assay run will affect cell usage. Higher-throughput assays will have lower Z' but reduced cell consumption on a per-well basis. Table 2 summarizes throughput and cell usage for CHO-m1 agonist and antagonist assays.

9. Confirm Z': % Activity
Stability vs. Time over URT. Once assay conditions had been set, the assay was validated. Typical validation criteria include data quality (Z') assessment and assay stabil
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
2. Cis-Reporting System Monitors Intracellular Calcium Mobilization
3. Cis-Reporting System Monitors Intracellular Calcium Mobilization
4. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
5. FLIPR Calcium Assay Kit Datasheet
6. FLIPR Calcium 3 Assay Kit Datasheet
7. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
8. HEK293 Calcium Flux Assay
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
11. Using Validated siRNAs in Functional Genomic Assays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Calcium Flux Assays using the LabChip

(Date:7/30/2015)... -- AACC welcomed thousands of medical professionals and ... & Clinical Lab Expo in Atlanta ... revolutionary advancements in clinical testing technology and research ... diagnose patients quickly and accurately and make sure ... As of Wednesday, July 29, more than 17,500 ...
(Date:7/30/2015)... ... July 30, 2015 , ... ... a field clinical study of its canine osteoarthritis stem cell product, currently under ... (Kansas City, KS) and will be marketed in the US by Aratana. This ...
(Date:7/30/2015)... 30, 2015 HIGHLIGHTS:Q2 2015 Results ... , Reported sales were $697 million compared to ... grew organically by 8%, and changes in foreign currency ... sales by 1%. , By business unit, organic ... and 11% in SAFC Commercial. , Reported diluted ...
(Date:7/29/2015)... , July 30, 2015 ... la santé, publie ses résultats pour le premier ... commente les résultats. Visionner l,interview ... http://www.eurobusinessmedia.com/ceo-direct/sanofi/sanofi-h1-2015-results-interview-with-ceo-olivier-brandicourt?utm_source=ceo-direct&utm_medium=wire Au sommaire de ... - Moteurs de croissance - ...
Breaking Biology Technology:Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 2Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 3Innovative Research on Cancer, Heart Disease, and Infectious Diseases Draws More than 17,500 Attendees to 2015 AACC Annual Meeting 4VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 2Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 3Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 4Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 5Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 6Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 7Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 8Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 9Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 10Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 11Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 12Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 13Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 14Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 15Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 16Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 17Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 18Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 19Sigma-Aldrich (NASDAQ - SIAL) Reports Q2 2015 Sales Of $697 Million And Adjusted Diluted EPS Of $1.01 20
... – EMD Serono, Inc., an affiliate of Merck ... a complete response letter (CRL) from the U.S. ... drug application (NDA) for Cladribine Tablets, EMD Serono,s ... therapy for relapsing-remitting multiple sclerosis (MS). ...
... , the team have created a microscope which shatters the ... the diffraction limit of light. Previously, the standard optical ... millimetres clearly. But now, by combining an optical ... Manchester researchers can see 20 times smaller 50 nanometres ...
... object has put Canadian researchers at the National Institute for ... of World Records. Only one atom at its end ... man-made object. It is made of Tungsten and fabricated ... evaluated for its commercial potential., "We did not start out ...
Cached Biology Technology:EMD Serono Receives Complete Response Letter From FDA on Cladribine Tablets New Drug Application 2EMD Serono Receives Complete Response Letter From FDA on Cladribine Tablets New Drug Application 3EMD Serono Receives Complete Response Letter From FDA on Cladribine Tablets New Drug Application 4Microscope could 'solve the cause of viruses' 2
(Date:7/8/2015)... NEW YORK , July 8, 2015 ... cancer globally, and the fourth most fatal, with a ... year. The poor prognosis of pancreatic cancer patients has ... to treatment, which is not being met by the ... contains an array of products with varying molecule types ...
(Date:7/7/2015)... 2015 Research and ... of the "Capacitive Fingerprint Sensors Patent Landscape" ... this date, fingerprint sensing technology is the most ... are well developed. This patent landscape focuses on ... The domain of capacitive fingerprint sensors is closely ...
(Date:7/2/2015)... -- Research and Markets( http://www.researchandmarkets.com/research/jvft55/next_generation ) ... Biometrics Market by Application, Technology, Function & Geography - ... The next generation biometrics market is expected ... of 17.9% between 2015 and 2020 Travel ... Safran SA ( France ), ...
Breaking Biology News(10 mins):Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 2Frontier Pharma: Pancreatic Cancer - Identifying and Commercializing First-in-Class Innovation 3Worldwide Capacitive Fingerprint Sensors Patent Landscape Market Report 2015-2020 - Main IP Holders include Seiko Epson, AuthenTec, Sony and STMicroelectronics 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 2Next Generation Biometrics Market 2015 - Global Forecast to 2020 with Safran, NEC, 3M Cogent, Fujitsu & Suprema Dominating the $24 Billion Market 3
... midge larvae ( Chaoborus spp.) may explain a few ... acidification and fish death in Swedish lakes. Researchers at the ... lake-bottom sediments to show when and how fish life disappeared ... acidified regions. "It is actually just like a ...
... Although the apple genome has already been sequenced, it can ... actual piece of fruit on a tree. That,s why University of ... help sequence the genome of the woodland strawberry a close ... making his work much more efficient. "The woodland ...
... study of 27,000 individual trees by National Science Foundation ... to produce viable seeds--are more sensitive to climate change ... the journal Global Change Biology , identify earlier ... tree reproduction and growth. They also ...
Cached Biology News:Dead midges reveal living conditions of fish 2Simpler woodland strawberry genome aids research on more complex fruits 2Simpler woodland strawberry genome aids research on more complex fruits 3'Epidemiological' study demonstrates climate change effects on forests 2
... and negatively supercoiled DNA in presence of EDTA ... effects of supercoiling on transcription in vitro ... Determining the degree of supercoiling of naturally occurring ... in length by only one base-pair , ...
Required to perform reference file generation, flatfield calibration and performance verification processes on the GSD-501...
Applications: Immunohistochemistry, ISH, FISH, CISH....
Applications: Immunohistochemistry, ISH, FISH, CISH....
Biology Products: