Navigation Links
Calcium Flux Assays using the LabChip 3000

ak calcium response yields maximum sensitivity to low agonist doses while maintaining high responses to high agonist doses (Figure 3).

5. Determine On-Chip Agonist Concentration
For antagonist assays, the concentration of agonist to be placed on the chip is determined. The CHO-m1 assay was run with on-chip agonist concentration equal to the compound EC80.

EC80 is determined by placing increasing concentrations of agonist in the wells of a microplate and determining cell response. This response is compared to a control response such as ionomycin and a % activity is calculated. Analysis of the dose-response curve (Figure 4) yielded an EC50 of ~ 0.07 μM and an EC80 of ~ 0.6 μM. The concentration of agonist in the agonist well on the chip was then set at 6 μM to yield 0.6 μM in the reaction channel of the chip.

6. Determine Compound Plate and 100% Control Concentrations
Compound concentrations in microplates and control troughs must be chosen to account for on-chip dilution from cell sidearms and dispersion in the main channel. Sidearm dilutions for agonist and antagonist chips is ~50%. Sample dilution due to dispersion depends on assay parameters such as sample sip time, detection zone and applied pressure. For agonist and antagonist chips, typical operating conditions of -2 psi, (zone 1), and sample sip times > 5 s result in effectively no dispersion-related dilution of compound from the sipper. On-plate and 100% control compound concentrations were therefore doubled to account for on-chip dilutions.

7. Assay Stability
Once reaction conditions have been determined, the stability of the assay over the anticipated run time can be assessed. Typical run times for cell-based assays range from 4 to 8 hr. Stabili
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
2. Cis-Reporting System Monitors Intracellular Calcium Mobilization
3. Cis-Reporting System Monitors Intracellular Calcium Mobilization
4. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
5. FLIPR Calcium Assay Kit Datasheet
6. FLIPR Calcium 3 Assay Kit Datasheet
7. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
8. HEK293 Calcium Flux Assay
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
11. Using Validated siRNAs in Functional Genomic Assays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Calcium Flux Assays using the LabChip

(Date:5/22/2013)... May 22, 2013  Superior Controls, Inc. has ... by Business NH Magazine.  For the past 22 ... that distinguish themselves with extraordinary business and civic ... receive this honor," said Rick Pierro ... any recognition, this award is a reflection of ...
(Date:5/22/2013)... -- Cleveland BioLabs, Inc. (NASDAQ: CBLI) today announced that the Company ... Microcap Conference, May 30, in New York, NY ... , CPA, Chief Financial Officer of Cleveland BioLabs, will present ... 1:30 p.m. Eastern Time.  A live and archived webcast of ... of the Cleveland BioLabs website at www.cbiolabs.com . ...
(Date:5/22/2013)... 2013 Express Diagnostics Int’l, Inc. ... are pleased to announce a sponsorship agreement with ... New Zealand distributor of DrugCheck® onsite testing devices, manufactured ... in the Austin 400 May 17-19 in Austin, Texas. ... that involves each car of the Erebus Motorsport team ...
(Date:5/22/2013)... 22, 2013 A recent study from ... athletes to find that those who took probiotics were able ... from the study showed that in extreme heat conditions, probiotics ... their adult acne treatment explains what this new ... may help their skin condition. , While researchers tote ...
Breaking Biology Technology:Superior Controls of Seabrook, NH named Business of the Year for 2013 by Business NH Magazine 2Cleveland BioLabs to Present at Second Annual Marcum LLP Microcap Conference 2Australian Distributor of Express Diagnostics Signs Sponsorship Agreement with V8 Supercar Team 2Adult Acne Treatment, Probiotic Action Shares Insight on New Findings Between Probiotics and Heat Tolerance, and What It Means for Acne Sufferers 2
... 18 Volcano Corporation,(Nasdaq: VOLC ), ... measurement (FM) products designed to enhance the ... disease, announced today that it,has completed its ... CardioSpectra is a privately-held company developing innovative ...
... Dec. 18 Pharmos Corporation,(Nasdaq: PARS ) ... has elected,to resign from the Company., Mr. ... instrumental in the,strategic acquisition and the subsequent integration ... Vela acquisition,Mr. Rubino has been focused on key ...
... Vice President and General Manager of facility ... prescriptions a week, FRANKLIN LAKES, N.J., Dec. 18 ... nation,s leading pharmacy benefit,managers, today announced that AllPoints at Anson ... most-advanced automated pharmacy,capping a nearly year-long search that spanned 48 ...
Cached Biology Technology:Volcano Announces Closing of CardioSpectra Acquisition 2Volcano Announces Closing of CardioSpectra Acquisition 3Pharmos Corporation Announces President & COO Alan L. Rubino to Leave Company 2Medco Chooses AllPoints at Anson in Whitestown, Indiana as Site for the World's Largest, Most-Advanced Automated Pharmacy 2Medco Chooses AllPoints at Anson in Whitestown, Indiana as Site for the World's Largest, Most-Advanced Automated Pharmacy 3Medco Chooses AllPoints at Anson in Whitestown, Indiana as Site for the World's Largest, Most-Advanced Automated Pharmacy 4Medco Chooses AllPoints at Anson in Whitestown, Indiana as Site for the World's Largest, Most-Advanced Automated Pharmacy 5
(Date:5/22/2013)... life scientists provide important new details on how climate ... online May 21 in the Journal of Animal ... making accurate predictions and informing policymakers of how species ... , "There is a growing recognition among biologists ... one another, and that this is going to have ...
(Date:5/21/2013)... NC Biologists have known for a long time ... why isn,t well understood, particularly for plants. But it ... Lanfear of Australian National University and the U. S. ... to be published 21 May in the journal ... plants have faster-changing genomes. , Drawing from a database ...
(Date:5/21/2013)... When infections occur in the body, stem cells ... multiplying and differentiating into mature immune cells that ... inflammation can deplete these cell populations, potentially leading ... as cancer. Now, a team of researchers led ... (Caltech) has found that, in mouse models, the ...
Breaking Biology News(10 mins):UCLA life scientists present new insights on climate change and species interactions 2UCLA life scientists present new insights on climate change and species interactions 3UCLA life scientists present new insights on climate change and species interactions 4UCLA life scientists present new insights on climate change and species interactions 5Small but speedy: Short plants live in the evolutionary fast lane 2Keeping stem cells strong 2
... involves the incorporation of community service into a course ... service learning model, students participate in ,real life, and ... T.M. Waliczek and J.M. Zajicek reported on a study ... in HortTechnology . The team found that involvement ...
... HOUSTON (June 23, 2011) The remoteness ... to astronaut health care. One solution is ultrasound. ... (NSBRI) have developed tools that expand the use of ... and underserved locations. These tools include techniques that streamline ...
... DALLAS June 23, 2011 UT Southwestern ... inhibit a signaling pathway in Huntington,s disease, a finding ... to help slow the progression of degenerative nerve disorders. ... for Huntington,s disease treatment and possibly for other neurodegenerative ...
Cached Biology News:Service projects increase learning, social impact for undergrads 2Space research gives birth to new ultrasound tools for health care in orbit, on Earth 2Space research gives birth to new ultrasound tools for health care in orbit, on Earth 3Space research gives birth to new ultrasound tools for health care in orbit, on Earth 4Compound may provide drug therapy approach for Huntington's disease 2