Navigation Links
Calcium Flux Assays using the LabChip 3000

ak calcium response yields maximum sensitivity to low agonist doses while maintaining high responses to high agonist doses (Figure 3).

5. Determine On-Chip Agonist Concentration
For antagonist assays, the concentration of agonist to be placed on the chip is determined. The CHO-m1 assay was run with on-chip agonist concentration equal to the compound EC80.

EC80 is determined by placing increasing concentrations of agonist in the wells of a microplate and determining cell response. This response is compared to a control response such as ionomycin and a % activity is calculated. Analysis of the dose-response curve (Figure 4) yielded an EC50 of ~ 0.07 μM and an EC80 of ~ 0.6 μM. The concentration of agonist in the agonist well on the chip was then set at 6 μM to yield 0.6 μM in the reaction channel of the chip.

6. Determine Compound Plate and 100% Control Concentrations
Compound concentrations in microplates and control troughs must be chosen to account for on-chip dilution from cell sidearms and dispersion in the main channel. Sidearm dilutions for agonist and antagonist chips is ~50%. Sample dilution due to dispersion depends on assay parameters such as sample sip time, detection zone and applied pressure. For agonist and antagonist chips, typical operating conditions of -2 psi, (zone 1), and sample sip times > 5 s result in effectively no dispersion-related dilution of compound from the sipper. On-plate and 100% control compound concentrations were therefore doubled to account for on-chip dilutions.

7. Assay Stability
Once reaction conditions have been determined, the stability of the assay over the anticipated run time can be assessed. Typical run times for cell-based assays range from 4 to 8 hr. Stabili
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
2. Cis-Reporting System Monitors Intracellular Calcium Mobilization
3. Cis-Reporting System Monitors Intracellular Calcium Mobilization
4. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
5. FLIPR Calcium Assay Kit Datasheet
6. FLIPR Calcium 3 Assay Kit Datasheet
7. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
8. HEK293 Calcium Flux Assay
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
11. Using Validated siRNAs in Functional Genomic Assays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Calcium Flux Assays using the LabChip

(Date:12/17/2014)... December 16, 2014 The Activated ... 2013, which is why IBISWorld updated its original industry ... to benefit from an intensified focus on environmental policy. ... new emission standards for power plants and a range ... to IBISWorld Industry Analyst Sarah Kahn, “a range of ...
(Date:12/17/2014)... Washington, USA (PRWEB) December 16, 2014 ... Baum, manager at Philipps- Universität Marburg’s Department of ... and photonics, in the newly released SPIE ... “And never be too shy to ask questions.” ... (science, technology, engineering, and mathematics) occupations ranging from ...
(Date:12/17/2014)... BASKING RIDGE, N.J. (PRWEB) December 17, 2014 ... Ipsen (Euronext: IPN; ADR: IPSEY), ... 120 mg (referred to as Somatuline®) was approved ... for the treatment of gastroenteropancreatic neuroendocrine tumors (GEP-NETs) ... differentiated, locally advanced or metastatic disease to improve ...
(Date:12/17/2014)... 17, 2014 This report is ... Tubing industry. For an overview analysis, the report ... industry chain structure, industry overview, policy analysis, and ... industry has witnessed rapid development with key ... acquisitions. This report mentions quartz tubing upstream raw ...
Breaking Biology Technology:Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 2Activated Carbon Manufacturing in the US Industry Market Research Report Has Been Updated from IBISWorld 3Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 2Network, Multitask, Persevere: SPIE Women in Optics Planner Subjects Share from Experience 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 2Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 3Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 4Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 5Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 6Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 7Ipsen Announces the FDA Approval of Somatuline® Depot® (lanreotide) Injection for the Treatment of Gastroenteropancreatic Neuroendocrine Tumors 82020 Quartz Tubing Market: Global Development & Forecasts Analysis Now at DeepResearchReports.com 2
... ARBOR, Mich. and AMSTERDAM, Feb. 23, 2012 ... commercializing sample-specific pre-analytical processes to improve the ... analytical platforms, today announced a clinical supply ... its TransPLEX® whole genome RNA amplification technology.  ...
... Feb. 23, 2012  Amarantus BioSciences, Inc. (OTCBB: AMBS), ... therapeutic protein, today announced that Gerald E. Commissiong, ... business plan at the 2012 National Investment Banking ... at 12:25pm CST at Le Pavillon Hotel in ...
... Telome Health, Inc.™, a privately held biotechnology company focused on ... that co-founder and Nobel Laureate Elizabeth Blackburn has been awarded ... " I am very pleased and honored ... innovation, Silicon Valley, and to acknowledge the efforts of my ...
Cached Biology Technology:Rubicon Genomics and Agendia Sign Agreement for Use of TransPLEX® Whole Genome RNA Amplification Technology With Symphony™ Cancer Diagnostics 2Rubicon Genomics and Agendia Sign Agreement for Use of TransPLEX® Whole Genome RNA Amplification Technology With Symphony™ Cancer Diagnostics 3Amarantus BioSciences to Present at the 2012 National Investment Banking Association Conference in New Orleans 2Telome Health Co-founder and Nobel Laureate Elizabeth Blackburn Awarded Innovator of the Year 2
(Date:12/5/2014)... Dec. 4, 2014  Tute Genomics, a leader in ... in Series A1 funding led by UK-based Eurovestech. Peak ... in the investment round. "We are at ... adopts next-generation sequencing and seeks new approaches for the ... Robison , MD MBA, and CEO of Tute Genomics. ...
(Date:12/3/2014)... , Dec. 2, 2014   Marvin ... deployed, innovative test solutions for military, aerospace, and ... version of its successful TS-900 PXI semiconductor ... and features of high-end systems to customers at ... value compared to traditional ATE. ...
(Date:11/21/2014)... According to a new market research report ... Control, Intrusion Detection, Parking Management, Under Vehicle Inspection), End ... - Global Forecasts to 2020", published by MarketsandMarkets, The ... $25 Billion in 2014 and is expected to reach ... 8.69%. Browse 116 market data Tables and ...
Breaking Biology News(10 mins):Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 2Tute Genomics Raises $2.3 Million to Scale Up Cloud-Based Genome Analytics and Accelerate Personalized Medicine 3Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3
... biocompatible, about 7 millimeters long and, incredibly, ... at the University of Illinois are making tracks ... has been a riddle that scientists at the ... solve. The walking bio-bots demonstrate the Illinois team,s ...
... 2012 ActiveCare, Inc. (OTC.BB: ACAR), the ... a dramatic decrease in diabetic claims in the first ... revolutionary health monitoring and information technology combined with a ... healthier Caprock. By collecting real-time biometric readings ActiveCare is ...
... available in German . Researchers from Jena ... of land crab migration on Christmas Island. In cooperation with ... scientists from the Max Planck Institute for Chemical Ecology in ... of freely roaming robber crabs, which is the first large-scale ...
Cached Biology News:These bots were made for walking: Cells power biological machines 2Diabetes Management Provider ActiveCare Announces Cost Savings Of Over $4,000 Per Year Per Diabetic Member 2Researchers use GPS tracking to monitor crab behavior 2
Immunogen: Extract of pigmented melanoma metastases from lymph nodes. Storage: -20 C, Avoid Freeze/Thaw Cycles...
with GlutaMAXIL-glutamine...
alpha N-catenin (C-19)...
This STAT antibody sampler pack contains 8 monoclonal ,STAT antibodies....
Biology Products: