Navigation Links
Calcium Flux Assays using the LabChip 3000

rated and the pellet was loosened by flicking prior to resuspension in 3 mL of cell assay buffer at 3-5 x 106 cells/mL (note cell assay buffer may require optimization, but an initial buffer composition given in Section IV should suffice for early phase feasibility).

3. Confirm Cell Detection
Typically, once cells have been loaded with dye and suspended in solution, the cells are added to the cell wells on the microfluidic chip. The cells are then carried to the detection point in the chip by applying a vacuum to the waste wells. Cell fluorescence excited by a 488 nm laser is detected at both 530 (Fluo-4) and 685 (Fura Red) nm. A spike or peak in fluorescence is observed as each cell passes the detector. Robust signals display peak heights greater than ten times the baseline noise.

4. Determine Kinetics of Response
The LabChip 3000 calcium flux chip is designed such that calcium response for each cell is measured at a fixed incubation time. The incubation time is determined by the cell velocity and the length of the incubation channel. Cell velocity is a function of pressure applied to the chip, the chip temperature and the solution viscosity, while incubation channel length can be varied by selecting detection points at three locations (or zones) along the cell path. Table 1 lists the dependence of incubation time on pressure and detection zone. Typically pressures of -1.2, -2 and -3 psi are sufficient to gather statistics on optimum incubation time. In this experiment, a candidate agonist is sipped up onto the chip and contacted with cells. The ratio of fluorescence emission for each cell at 530 and 685 nm is measured and plotted as a function of incubation time. From this experiment 15 s (pressure = -2 psi at Zone 1) was determined to be the optimum incubation time for CHO-m1 cells stimulated by carbachol, since detecting at a point after the pe
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
2. Cis-Reporting System Monitors Intracellular Calcium Mobilization
3. Cis-Reporting System Monitors Intracellular Calcium Mobilization
4. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
5. FLIPR Calcium Assay Kit Datasheet
6. FLIPR Calcium 3 Assay Kit Datasheet
7. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
8. HEK293 Calcium Flux Assay
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
11. Using Validated siRNAs in Functional Genomic Assays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Calcium Flux Assays using the LabChip

(Date:7/10/2014)... CA (PRWEB) July 10, 2014 Senior ... institutions and professional associations, addressed the challenges of “Reducing ... Medical Device Industries” through strategic sourcing. Describing the partnership ... with the Supply Chain Management Institute ( SCMI) ... event on June 24 at the Kroc Institute of ...
(Date:7/10/2014)... , July 10, 2014 On ... Dmitry Medvedev , presented Russia,s ... BIOCAD . The Company is developing a unique project ... project BIOCAD develops a number of innovative drugs based ... diseases. The ceremony took place at the International Exhibition ...
(Date:7/10/2014)... , July 10, 2014 /PRNewswire-iReach/ -- Tute ... interpretation, today announced an agreement with Lineagen, ... for Lineagen,s NextStep Dx PLUS.  Lineagen, ... and neurological disorders, currently offers FirstStep Dx ... testing service used by healthcare providers as ...
(Date:7/10/2014)... July 10, 2014   Ceres, Inc . (Nasdaq: ... company, announced today financial results for the three months ... its business. Ceres reported that the company ... performance this growing season in Brazil, which concluded in ... company,s sorghum evaluation areas for part of the growing ...
Breaking Biology Technology:Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 2Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 3Biotech & Biopharm Supply Chain Management Executives Launch BSMA in San Diego 4Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9
... witnessed a biotech meltdown during the first quarter of 2005 ... will take a look at the larger cap life science ... for the pharmaceutical sector. You may have noticed headlines at ... decision to pull its Cox-2 inhibitor (Bextra) off the market. ...
... series that will follow entrepreneurs through the trials of starting ... finalists in the Governor's Business Plan Contest. Surgeons have little ... procedures. For Milwaukee-area general surgeon Michael Phillips, frustration with having ... out during cancer surgeries led to the creation of the ...
... me that if you couldn't say anything nice, don't say ... ,Occasionally something so egregious happens that it must be addressed. ... products. , ,What's got my steam vents working overtime is ... in a single pass." Oh my. What kind of business ...
Cached Biology Technology:Midwest large-cap life-science sector is solid in first quarter 2Vector Surgical hopes to turn frustration into faster surgeries 2Vector Surgical hopes to turn frustration into faster surgeries 3Double your productivity! Hype that damages technologists' reputations 2Double your productivity! Hype that damages technologists' reputations 3
(Date:7/11/2014)... normal circumstances, astrocytes participate in normal physiological ... exhibit therapeutic and repairing effects on brain ... found that nerve cells differentiated from adipose-derived ... viability, which produces influences on subsequent studies ... Hospital, Hebei United University, China demonstrated that ...
(Date:7/10/2014)... Santa Fe, Albuquerque, and other major cities in New ... with treated municipal wastewater rather than precious potable water ... than 40% of all golf courses receive treated effluent. ... , Additionally, golf courses and homeowners alike fertilize ... in fertilizer is nitrate. A New Mexico State University ...
(Date:7/10/2014)...  Unisys Corporation,s (NYSE: UIS ) subsidiary in ... announced that it has been selected by the Dutch Custodial ... Basic Provision Biometrics solution for penitentiaries across The ... with a solution based on its open standards-based LEIDA ... contract is up to seven years, with the new system ...
Breaking Biology News(10 mins):'Tailored' water -- the latest in lawn care 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 2Unisys Delivers New Basic Provision Biometrics Solution to Dutch Custodial Institutions Service 3
... in salads are chock-full of important vitamins and nutrients, ... type and amount of salad dressing, a Purdue University ... subjects salads topped off with saturated, monounsaturated and polyunsaturated ... fat-soluble carotenoids ╨ compounds such as lutein, lycopene, beta-carotene ...
... (June 19, 2012) Using data from its unique online ... to the finding of six novel genetic associations for early ... study now published online in the journal PLoS Genetics ... of new genetic discoveries. Nearly 90 percent of ...
... 2012 Digicel Pacific Limited, a mobile network operator across ... it is deploying biometric enabled payment systems into stores in ... more convenient way to send money home to the Pacific. ... home to register just once and record their fingerprint as ...
Cached Biology News:Study: No-fat, low-fat dressings don't get most nutrients out of salads 223andMe contributes to genetic discoveries related to male pattern baldness 223andMe contributes to genetic discoveries related to male pattern baldness 3Digicel Launches World's First Biometric Identification System For International Money Transfers 2Digicel Launches World's First Biometric Identification System For International Money Transfers 3
IHC detection kit for PCNA in cells and tissues...
... Immunogen: Synthetic peptide derived from ... WNT1 protein. Specificity: Reacts with ... Reactivity: Human Mouse (positive controls: ... cell lysates and mouse Mouse-1 cell ...
The pYES2 vector is designed for native expression of your protein of interest in S. cerevisiae. It contains the URA3 gene for selection in yeast and 2 origin for high-copy maintenance....
... [125I] Direct SPA Screening Assay, ... reagents that eliminate lengthy sample extraction ... (optional use).Range: 1.32-84.28 ng/ml (4-256 pmol/ml).Sensitivity: ... at 2-8 C. Category: Drug Screening ...
Biology Products: