Navigation Links
Calcium Flux Assays using the LabChip 3000

I. Introduction

A calcium flux assay has been developed for the LabChip 3000 microfluidic system for use in drug screening against GPCR targets. The miniaturized platform results in low cellular and reagent consumption, high data quality and a high degree of automation.

The LabChip microfluidic assay accesses test compounds from a microplate and mixes the samples with suspended cells continuously flowing through the chip. Flow through the microchannel network results from a vacuum applied to the waste wells of the chip. The system measures the fluorescence properties of individual cells flowing past a fluorescence detector. The LabChip system measures as few as 50 cells and uses less than 50 nL of sample and 15 nL of agonist per measurement.

The steps involved in developing and validating an assay to conduct screens for GPCR agonists and antagonists will be discussed here. CHO-m1 is used as a model cell line.

CHO-m1 Assay Summary
Typical cell usage as low as 600-1000 cells/well
Z' = 0.86 for Agonist, Z' = 0.72 for Antagonist
Unattended run time of between 4 and 8 hr
Throughput of up to 8,000 samples in 8 hr

II. Overview

The general steps followed in the development of a calcium flux assay are outlined in Figure 1. A preliminary choice of cell line, growth conditions, and cell assay buffer is made (Step 1). A protocol for labeling, washing and suspending cells is then developed (Step 2), after which confirmation is made that the labeled cells can be detected (Step 3). Once feasibility has been established, the kinetics and magnitude of response to agonist exposure is measured (step 4) and, in the case of an antagonist assay, the concentration of agonist to be placed on-chip is determined (Step 5).

The dilution factor used to calculate the appropriate microplate an
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
2. Cis-Reporting System Monitors Intracellular Calcium Mobilization
3. Cis-Reporting System Monitors Intracellular Calcium Mobilization
4. Calcium Plus Assay Kit, Superior Detection for Specialized Targets
5. FLIPR Calcium Assay Kit Datasheet
6. FLIPR Calcium 3 Assay Kit Datasheet
7. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
8. HEK293 Calcium Flux Assay
9. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
10. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
11. Using Validated siRNAs in Functional Genomic Assays
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Calcium Flux Assays using the LabChip

(Date:7/28/2014)... PA & Boston, MA (PRWEB) July 28, 2014 ... collaboration agreements with two researchers to further develop potential ... deadly malignant brain tumor, in combination with their patented ... by ITI and Duke University, are based on the ... Chief, Division of Neurosurgery at The Preston Robert Tisch ...
(Date:7/28/2014)... , July 28, 2014  InterMune, Inc. (NASDAQ: ... release its second quarter 2014 financial results at the ... 2014. A live conference call and webcast will be hosted ... day. Interested investors and others may participate ... +1 212-231-2900 (international), conference ID#21728775. A replay of the webcast ...
(Date:7/25/2014)... "Ischemic Stroke and Cerebral Stroke ... on the Ischemic Stroke and Cerebral Stroke clinical ... data relating to the clinical trials on Ischemic ... Ischemic Stroke Research Report 2014 at: http://www.marketresearchreports.biz/analysis/213207 ... trial numbers and their recruitment status as per ...
(Date:7/25/2014)... According to a new market report ... Global Industry Analysis, Size, Share, Growth, Trends and Forecast, ... estimated at USD 161,056.5 million in 2014 and is ... 2014 to 2020, to reach an estimated value of ... market is witnessing a significant growth due to the ...
Breaking Biology Technology:String of Collaborations Launch Immunomic Therapeutics’ Brain Tumor Program Pipeline 2String of Collaborations Launch Immunomic Therapeutics’ Brain Tumor Program Pipeline 3String of Collaborations Launch Immunomic Therapeutics’ Brain Tumor Program Pipeline 4InterMune to Release Second Quarter Financial Results on August 6 2Ischemic Stroke and Cerebral Stroke Global Clinical Trials Review H2, 2014: MarketResearchReports.Biz 2Ischemic Stroke and Cerebral Stroke Global Clinical Trials Review H2, 2014: MarketResearchReports.Biz 3Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 2Biological Drugs Market: Global Industry Analysis, Size, Share, Growth, Trends and Forecast 2014 - 2020 3
... 5 Cell Therapeutics (CTI),(Nasdaq and MTA: CTIC) announced ... Preferred Stock in exchange for 6,634 shares of CTI,s ... constitutes 83% in interest of all,series of CTI,s Convertible ... become convertible into 7,143 shares of CTI,s common stock, ...
... DIEGO, Feb. 4 Orexigen(R) Therapeutics, Inc.,(Nasdaq: OREX ), ... the Company will be speaking at the 11th Annual,BIO CEO & ... Corporate Presentation:, Date: Monday, February 9, ... Eastern, Location: Waldorf Astoria, Astor Room, New York, ...
... Feb. 4 NeurogesX, Inc. (Nasdaq: NGSX ... novel pain management therapies, announced today that Anthony DiTonno, ... at the 11th Annual BIO CEO & Investor Conference ... Mr. DiTonno and Stephen Ghiglieri, Chief Financial Officer, will ...
Cached Biology Technology:Cell Therapeutics Exchanges 83% of Remaining Convertible Preferred Stock Into New Non-dividend Bearing, Non-redeemable Convertible Preferred Stock With Conversion Price of $0.14 per Share 2NeurogesX to Present at 11th Annual BIO CEO & Investor Conference 2NeurogesX to Present at 11th Annual BIO CEO & Investor Conference 3
(Date:7/28/2014)... top 10 U.S. cities that have seen an increase ... frequent road closures, overwhelmed storm drains and compromised infrastructure--are ... technical report. , This nuisance flooding, caused by rising ... between 300 and 925 percent since the 1960s. , ... Changes around the United States, also finds Annapolis and ...
(Date:7/28/2014)... The findings of a new study suggest two ways ... not work as well in obese women, compared to women ... are a one-size-fits-all method, researchers say, but as the population ... the pill works for obese women. Studies have consistently found ... the body, which may in turn affect how well the ...
(Date:7/28/2014)... He calls himself the bug hunter, but the ... only be found and identified with special methods and ... Kansas State Veterinary Diagnostic Laboratory at Kansas State University, ... porcine enterovirus G, which is an important find in ... in cells, but didn,t know what it was," Hause ...
Breaking Biology News(10 mins):NOAA: 'Nuisance flooding' an increasing problem as coastal sea levels rise 2NOAA: 'Nuisance flooding' an increasing problem as coastal sea levels rise 3Strategies identified to improve oral contraceptive success with obese women 2Researcher using next-generation sequencing to rapidly identify pathogens 2Researcher using next-generation sequencing to rapidly identify pathogens 3
... Kan. -- With harvest in full swing, many farmers have ... and minimal rainfall has left most wheat yields looking less ... ugly. But as a Kansas State University plant pathologist ... also has been battling a much smaller opponent: viruses. ...
... to be an acclaimed field biologist and college dean is ... Joseph Travis, dean of the College of Arts and Sciences ... researcher in the field of ecological genetics, has won the ... of the ASN in June, the award "is given to ...
... 2011 , the New York Academy of Sciences will ... of subspecialties, in addition to patients and caregivers, to ... of the clinical features, management, and treatment of Shwachman-Diamond ... affecting children and young adults. The clinical features ...
Cached Biology News:Plant pathologist finding Kansas wheat fields a molecular battleground this season 2FSU dean wins prestigious E.O. Wilson Naturalist Award 2FSU dean wins prestigious E.O. Wilson Naturalist Award 3New York Academy of Sciences hosts a conference on rare disease Shwachman-Diamond Syndrome 2
...
Normal human serum collected off the clot from healthy normal humans. Each donor unit is negative for anti-HIV 1&2, HIV-Ag, HCV, and HBsAg by FDA required tests...
Normal human AB serum, pooled...
sterile filtered, off the clot, all male donors, Total Protein: 4.5 to 8.5 g/dl...
Biology Products: