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COSMCPREP HIGH AND LOW COPY PLASMID PURIFICATION

b> HIGH COPY PLASMID PURIFICATION
CosMCPrep successfully purifies plasmids derived from a number of vectors propagated in a variety of bacteria, including DH5a, DH10B and JM101. Figure 4 shows the products of a CosMCPrep purification from nine plasmid clones. The high quality of CosMCPrep-purified plasmids is demonstrated by the quality of sequencing results, such as pass rates in excess of 90% and Phred20 values routinely above 650bp.
COSMCPREP AND THE RICKETTSIA GENOME PROJECT
One of CosMCPreps unique features is the ability to use a single reagent system to purify a variety of template types. CosMCPrep was exclusively used as the template purification method for the Rickettsia Genome Sequencing Project performed at Agencourt. The complete automation of CosMCPrep enabled Agencourt to complete all sequencing in under four days.
SEQUENCING STRATEGY
Genomic DNA was cloned into both fosmid (pEPI-FOS57.5kb single copy per cell vector; 35-40kb inserts) and low copy plasmid vectors (pBT3.2kb plasmid; 5-10 copies per cell) for sequencing and functional genomic studies. More than 30,000 sequencing reactions were run from the purified templates. The low copy plasmid sequencing results, shown in Figure 5, had average Phred20 bases in excess of 640 and an average pass rate of 90%.

COMPLETE COSMCPREP AUTOMATION
CosMCPreps streamlined proto
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