Navigation Links

CosMCPrep is a versatile SPRI-based, plasmid purification system that uses a single protocol to purify a variety of high and low copy number template types. This flexible system is easily automated and produces high quality templates. The single protocol, ease of automation and high quality results make CosMCPrep an ideal solution for all template purification needs.

  • High quality plasmid purification
  • Single protocol for all template types
  • Fully automated protocols
  • No centrifugation or filtration steps
  • Superior downstream sequencing results


    CosMCPrep can be used for purification of:
  • BACs
  • Cosmids
  • Fosmids
  • Low copy plasmids
  • High copy plasmids


    Purification of low copy plasmids can be challenging due to the low numbers per cell. CosMCPrep enables efficient, fully-automated purification of low copy plasmids such as fosmids (Figure 1) and BACs. CosMCPrep-purified plasmids are high quality and result in improved sequencing performance (Figure 2). Figure 3 shows a BAC end sequence with a Phred20 read length of 700bp.

    CosMCPrep successfully purifies plasmids derived from a number of vectors propagated in a variety of bacteria, including DH5a, DH10B and JM101. Figure 4 shows the products of a CosMCPrep purification from nine plasmid clones. The high quality of CosMCPrep-purified plasmids is demonstrated by the quality of sequencing results, such as pass rates in excess of 90% and Phred20 values routinely above 650bp.
    One of CosMCPreps unique features is the ability to use a single reagent system to purify a variety of template types. CosMCPrep was exclusively used as the template purification method for the Rickettsia Genome Sequencing Project performed at Agencourt. The complete automation of CosMCPrep enabled Agencourt to complete all sequencing in under four days.
    Genomic DNA was cloned into both fosmid (pEPI-FOS57.5kb single copy per cell vector; 35-40kb inserts) and low copy plasmid vectors (pBT3.2kb plasmid; 5-10 copies per cell) for sequencing and functional genomic studies. More than 30,000 sequencing reactions were run from the purified templates. The low copy plasmid sequencing results, shown in Figure 5, had average Phred20 bases in excess of 640 and an average pass rate of 90%.

    CosMCPreps streamlined protocol does not require the labor-intensive process of cellular debris removal making CosMCPrep a fully-automated plasmid purification system. Automation of CosMCPrep on medium or high throughput robotic platforms has proven to quickly and cost effectively purify high quality plasmids (Figure 6).

  • '"/>


    Page: All 1 2 3

    Related biology technology :

    Post Your Comments:

    (Date:7/22/2014)... LANSING, Mich., July 22, 2014  Neogen Corporation (NASDAQ: ... its 2014 fiscal year, which ended May 31, increased ... net income was $28,158,000, compared to the prior year,s ... 30, 2013, earnings per share in the current year ... revenues and net income for the 2014 fiscal year ...
    (Date:7/22/2014)... , July 22, 2014 Hussey Copper ... MD-Cu 29 antimicrobial copper announces Pullman Regional Hospital,s ... first EPA-registered antimicrobial solid touch surface, at a nominal ... of MRSA, E-coli and other bacteria within 2 hours of ... protecting against bacteria and infectious diseases in its facilities. ...
    (Date:7/22/2014)... July 22, 2014  United Therapeutics Corporation (NASDAQ: ... will release its second quarter 2014 financial results ... United Therapeutics will host a half-hour ... a.m. Eastern Time.  The teleconference is accessible by ... rebroadcast of the teleconference will be available for ...
    (Date:7/22/2014)... (PRWEB) July 22, 2014 Dr. Williams ... those who have recognized that the greatest health care ... far too often needlessly deadly while trying to serve ... of quasi-experts seeking to transform an enormous system that ... Dr. Williams, in his continuing series of Open Letters ...
    Breaking Biology Technology:Neogen reports 19% increase in annual revenues 2Neogen reports 19% increase in annual revenues 3Neogen reports 19% increase in annual revenues 4Neogen reports 19% increase in annual revenues 5Neogen reports 19% increase in annual revenues 6Neogen reports 19% increase in annual revenues 7Neogen reports 19% increase in annual revenues 8Pullman Regional Hospital changes more than 1,100 touch points to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 2Pullman Regional Hospital changes more than 1,100 touch points to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 3Dr. Ira Williams Asks What’s Wrong With Jonathan Bush’s Book, Where Does It Hurt? 2
    ... Gov. Jim Doyle , whose "Grow Wisconsin" economic development ... state, has signed a bill that removes from the ... to capital formation , ,With little fanfare, Doyle signed ... the Wisconsin Economic Development Association. The law repeals a ...
    ... Something that was always taken for granted was the quality ... Sprint commercials, they emphasized that "you can hear a pin ... infrastructure. , ,The old Bell system also had very ... from analog to digital lines with digital repeaters (amplifiers) that ...
    ... Wis. - Tundra Semiconductor Corp. , the ... and computing markets, has announced the acquisition of ... company based in Eau Claire, for $14 million. , ... as a division of Tundra Semiconductor Corp. The company ...
    Cached Biology Technology:Doyle repeals shareholder liability 2Is traditional voice quality an oxymoron with today's cellular, VoIP? 2Is traditional voice quality an oxymoron with today's cellular, VoIP? 3Canadian firm acquires Silicon Logic 2
    (Date:7/22/2014)... July 22, 2014 BGI Tech Solutions Co., ... world,s largest genomics organization, announced today the global ... service based on Complete Genomics, industry-leading platform. This ... SNP validation, and is available at reduced prices ... highly regarded in the industry for providing proprietary ...
    (Date:7/22/2014)... spot with natural tourism resources, a recent study revealed a ... Geoscience and Mineral Resources (KIGAM) indicated that there are the ... 5,000 years ago. That is the first time to actually ... volcano 5,000 years ago in the inland part of the ... The research team led by Dr. Jin-Young Lee confirmed in ...
    (Date:7/22/2014)... type of immune cell widely believed to exacerbate ... and multiple sclerosis (MS), can actually protect the ... slow the progression of neurodegenerative diseases, according to ... journal Nature Communications . , The research ... the Department of Neurosciences at Cleveland Clinic,s Lerner ...
    Breaking Biology News(10 mins):BGI Tech launches human whole exome sequencing service on complete genomics' advanced platform 2Jeju Island is a live volcano 2Cleveland Clinic researchers discover neuroprotective role of immune cell 2
    ... Bio-Technology Group Co., Inc. (OTC Bulletin Board: ... leading developer, manufacturer and marketer of Traditional Chinese ... other health and nutritional products in the People,s ... its private placement offering for aggregate gross proceeds ...
    ... In order for medical students to ultimately provide quality patient ... teach physiology, one of the core disciplines of medicine, according ... Journal of the American College of Radiology ( ... systems. "It is vital that medical schools provide ...
    ... Avantra Biosciences (Avantra) today announced the selection ... test site for Avantra,s new biomarker quantitation platform. ... has created an automated multiplex immunoassay system to ... patient characteristics, and drug responses. The new Q400 ...
    Cached Biology News:Weikang Bio-Technology Announces Completion of $5.0 Million Private Placement 2Weikang Bio-Technology Announces Completion of $5.0 Million Private Placement 3Avantra Biosciences teams with TGen Drug Development to assist pharma 2
    BD IMag Anti-Mouse CD90.2 (Thy1.2) Particles-DM 2 x 109 cells...
    Chicken polyclonal to YebU ( Abpromise for all tested applications). Antigen: Synthetic peptide, corresponding to amino acids 196-244 of E. coli YebU....
    Mouse monoclonal [6911] to E. coli K99 pilus ( Abpromise for all tested applications)....
    ... Rabbit Antibody to CRF ... with the ovine CRF sequence ... Cells and fibres are found ... of rat brain in colchicine ...
    Biology Products: