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CLEANSEQ DYE-TERMINATOR REMOVAL

CleanSEQ is a SPRI-based dye-terminator removal system with a simple, three-step protocol requiring no centrifugation or filtration steps. CleanSEQ produces superior quality sequencing results and is easily automated, making it the preferred dye-terminator removal method in many of the top genomic research facilities.

HIGH QUALITY SEQUENCING RESULTS

CleanSEQ consistently delivers:

  • Long Phred20 read lengths
  • Increased fluorescent signal strength
  • Efficient elimination of unincorporated dyes (Figure 1)

  • FLEXIBLE PURIFICATION
    CleanSEQ is compatible with common sequencing chemistries and platforms in both 96 and 384-well formats.

    PLATFORMS:
  • ABI Prism 3730, 3700 and 3100
  • Beckman CEQ
  • Amersham MegaBACE

    REAGENTS:
  • BigDye versions 1.0, 1.1, 2.0, 3.0 and 3.1
  • DYEnamic ET
  • CEQ DTCS



    COMPLETE CLEANSEQ AUTOMATION
    The lack of centrifugation and filtration steps allows CleanSEQ protocols to be completely automated while maintaining superior performance. CleanSEQ is fully compatible with most commonly used low, medium and high throughput liquid handling systems. Contact Agencourt for the most up-to-date list of supported platforms.



    CleanSEQ has been successfully automated on the Biomek FX from Beckman Coulter in 96 and 384-well formats. (Photo courtesy of Beckman Coulter.)




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