Navigation Links
CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

page.

PCU Setup: (DO loop)
DO Control Selections: Auto (4 Gas mode)
PID values: P = 2.00 and I = 0.67

Gas Control (O2 loop)
The control was set to 4-Gas mode, to automatically maintain DO and pH setpoints, using 3 gases (Air, O2, N2) to control the DO setpoint and CO2 to control pH setpoint.

PCU Setup: (O2 loop)
O2 Control Selections: 4 Gas mode

Continuous Feed
All pumps were calibrated using the standard tubing supplied to keep track of the liquid quantities entering and exiting the vessel.

Samples were taken several times a day for off-line measurement of glucose and cell density.

PCU setup: (Pump 1 & Pump 2 loop)
Pump 1 . . . . . . . . . . .. .plugged into "Pump A" Power Controller outlet
Pump 2 . . . . . . . . . .. . .plugged into "Pump B" Power Controller outlet
Pump 1 (Nutrient) . .. ..Vessel inlet - Triport adapter in the vessel headplate
Pump 2 (Harvest) . . . .Vessel outlet - 12mm port using the dip tube
Pump A Selection . .. . .Manual mode (automatically adds liquid to the vessel with the BioCommand control program)
Pump B Selection . .. . .Wet On (removes liquid from the vessel using the level probe as a trigger to turn the pump on)

Control Program
For this study, we used NBS BioCommand Plus software to control nutrient addition. For the nutrient control program, we calculated the approximate time frame profile to automatically add fresh media using pump 1.




'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Big Blue Rodent Cell Cultures for Mutagenesis Studies
2. Simple Isolation of RNA from Tissue and Cultured Cells
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. The QIAGEN Guide to Animal Cell Culture
7. The QIAGEN Guide to Animal Cell Culture
8. The QIAGEN Guide to Animal Cell Culture
9. RNAi in Non-Mammalian Cultured Cells
10. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: CHO Cell Culture Application Using BioFlo Benchtop Bioreactor

(Date:7/30/2015)... ... July 30, 2015 , ... ... a field clinical study of its canine osteoarthritis stem cell product, currently under ... (Kansas City, KS) and will be marketed in the US by Aratana. This ...
(Date:7/30/2015)... COPENHAGEN, Denmark , July 30, 2015 /PRNewswire/ ... a biotechnology company that applies its innovative TransCon ... announced positive top-line results from a six-month Phase ... of once-weekly TransCon Growth Hormone in 53 treatment-naïve, ... "We are extremely pleased with the ...
(Date:7/30/2015)... ... July 30, 2015 , ... ... applications and designed for continuous operation up to 1500 bar. The sanitary design ... efficiency, and preventing toxic contamination. , The Pony™ NS2006L homogenizer is an ideal ...
(Date:7/29/2015)... US-Australian drug discovery company, Novogen Limited (ASX: ... committed to progressing its ground-breaking technology platforms to Phase ... ensure the Company delivers the best value for shareholders. ... , said the Company currently had an extensive program ... programs and academic partnerships and initiatives, and he was ...
Breaking Biology Technology:VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 2VetStem Biopharma Successfully Completes Large Milestone Efficacy Study for its Flagship Canine Osteoarthritis Stem Cell Product 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 5Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 6Ascendis Pharma A/S Announces Positive Top-line Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 7GEA Announces the Pony™ NS2006L… a Self-contained, High Pressure Lab Homogenizer for Product Development Ensuring Maximum Efficiency and Safety 2Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 2Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 3Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 4Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 5Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 6Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 7Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 8Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 9Newly Appointed Acting CEO Outlines Immediate Plans for Novogen 10
... SAN DIEGO, Dec. 13 BioMed Realty Trust, Inc.,(NYSE: ... I. Gilchrist to its,board of directors, increasing the board ... in the real estate industry, with a breadth of,ability ... large-scale commercial real estate projects. We believe that,Rick,s contributions ...
... Lesson as Prep for ... In-Person ... physician,comfort and knowledge with techniques around botulinum toxin injections,lead to ... Inc. (Solstice) has created MYOBLOC(TM) University, an,online, self-study resource that ...
... and PHILADELPHIA, December 13, The Board of Directors ... the global specialty biopharmaceutical company,today announces its Board ... retire as Non-Executive Chairman; Matthew Emmens (56) will ... be appointed Chief,Executive., These Board changes will ...
Cached Biology Technology:BioMed Realty Trust Appoints Executive Officer of the Irvine Company to Its Board 2BioMed Realty Trust Appoints Executive Officer of the Irvine Company to Its Board 3New Online Learning Tool Fills the Knowledge Gap on Botulinum Toxin Type B for Cervical Dystonia 2New Online Learning Tool Fills the Knowledge Gap on Botulinum Toxin Type B for Cervical Dystonia 3Shire plc: Board Changes 2Shire plc: Board Changes 3Shire plc: Board Changes 4
(Date:7/31/2015)... China , 31 de julho de 2015 /PRNewswire/ ... (ICG-10, www.icg-10.org ) será realizada pela BGI de 22-25 ... . A conferência está celebrando seu ... em 2006, a ICG se tornou uma das reuniões ... um dos encontros científicos mais dinâmicos, entusiastas e prazerosos. ...
(Date:7/31/2015)... Die 10. internationale Konferenz zu Genomik ... und findet vom 22. bis 25. Oktober 2015 ... Die Konferenz feiert in diesem ... ist die ICG weltweit zu einem der einflussreichsten ... eines der dynamischsten, enthusiastischsten und angenehmsten wissenschaftlichen Zusammentreffen. ...
(Date:7/30/2015)... 2015 Cellecta, Inc., a U.S.-based provider ... and biomarker discovery, announced the release of its ... all human protein coding genes. CRISPR technology enables ... gene,s function. Cellecta,s new Human Whole Genome CRISPR ... so that researchers can investigate in a single ...
Breaking Biology News(10 mins):A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 2A 10a. Conferência Internacional sobre Genômica (ICG-10) será em outubro 3Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 2Die 10. internationale Konferenz zu Genomik (ICG-10) beginnt im Oktober 3CELLECTA, INC. Announces Launch of Human Whole Genome CRISPR Knockout Library 2
... the University of Toronto describes a nanoscale material they've ... smaller digital devices and cellphones, and could even lead ... , The material, known as periodic mesoporous organosilica (PMO), ... of a metre across. The team created it by ...
... a molecular switch in mammals that shifts the body's fuel ... torpor in mice, a research team led by scientists at ... the Jan. 19 edition of Nature. , While ... note that the pivotal metabolic signal that emerged from the ...
... mother counts her newborn's fingers and toes, she probably ... baby's complete set of 20 digits. , Although ... substance responsible for clogging arteries, Vanderbilt University Medical Center ... an important developmental protein controls the development of fingers ...
Cached Biology News:Darkness unveils vital metabolic fuel switch between sugar and fat 2Darkness unveils vital metabolic fuel switch between sugar and fat 3Cholesterol gets 'thumbs up' for role in digit development 2Cholesterol gets 'thumbs up' for role in digit development 3
Mouse monoclonal [Ley 37D/G6] to LKB1 ( Abpromise for all tested applications). entrezGeneID: 6794 SwissProtID: Q15831...
... phase reagent for Virus ... & unique water insoluble ... detergents and chaotropes; Engineered ... non-envelop viruses while preserving ...
Integrins Sampler Kit 10g each...
PKC Sampler Kit 10 mu g each...
Biology Products: