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CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

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PCU Setup: (DO loop)
DO Control Selections: Auto (4 Gas mode)
PID values: P = 2.00 and I = 0.67

Gas Control (O2 loop)
The control was set to 4-Gas mode, to automatically maintain DO and pH setpoints, using 3 gases (Air, O2, N2) to control the DO setpoint and CO2 to control pH setpoint.

PCU Setup: (O2 loop)
O2 Control Selections: 4 Gas mode

Continuous Feed
All pumps were calibrated using the standard tubing supplied to keep track of the liquid quantities entering and exiting the vessel.

Samples were taken several times a day for off-line measurement of glucose and cell density.

PCU setup: (Pump 1 & Pump 2 loop)
Pump 1 . . . . . . . . . . .. .plugged into "Pump A" Power Controller outlet
Pump 2 . . . . . . . . . .. . .plugged into "Pump B" Power Controller outlet
Pump 1 (Nutrient) . .. ..Vessel inlet - Triport adapter in the vessel headplate
Pump 2 (Harvest) . . . .Vessel outlet - 12mm port using the dip tube
Pump A Selection . .. . .Manual mode (automatically adds liquid to the vessel with the BioCommand control program)
Pump B Selection . .. . .Wet On (removes liquid from the vessel using the level probe as a trigger to turn the pump on)

Control Program
For this study, we used NBS BioCommand Plus software to control nutrient addition. For the nutrient control program, we calculated the approximate time frame profile to automatically add fresh media using pump 1.




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