Navigation Links
CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

ulation. The DO loops Current Value remained higher than setpoint until the culture was introduced.
Temperature . . . . . . . . . . . . . . . .37C
pH . . . . . . . . . . . . . . . . . . . . . . . .7.2
DO . . . . . . . . . . . . . . . . . . . . . . . .50%
Agitation . . . . . . . . . . . . . . . . . . .70-130 rpm
O2 (Gases) . . . . . . . . . . . . . . . . . .4 Gas

PH Control
The pH probe was calibrated prior to the autoclave cycle (refer to BioFlo 110 Instruction Manual). CO2 gas and a liquid base were used to maintain the pH setpoint. pH control parameters were:
Base . . . . . . . . .. . .Sodium bicarbonate, 8% solution
Pump . . . . . . .. . . .Pump 4 of the 4-Pump Module
Transfer tubing . . .Narrow bore silicone tubing, as supplied (116" ID & 316" OD - NBS Catalog # P0740-2396)
Vessel inlet . . . . .. .Triport adapter in the vessel headplate

PCU setup: (pH loop)
Pump 4 plugged into "BASE" Power Controller Outlet
pH Control Selections:
Auto mode
Multiplier = 100% until base feed is started, after inoculation. Then, 25%
Multiplier = 25% after inoculation
Dead-band = 0.1
PID values: factory defaults

Dissolved Oxygen (DO) Control
The DO probe was calibrated after the autoclave cycle (refer to BioFlo 110 Instruction Manual). DO control was set to Auto mode, which automatically defaults to 4-Gas Mode after the Gas Control (O2 loop) selection is made. We also changed the P and I value of the DO loop to meet the culture demands, as shown on the next
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Big Blue Rodent Cell Cultures for Mutagenesis Studies
2. Simple Isolation of RNA from Tissue and Cultured Cells
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. The QIAGEN Guide to Animal Cell Culture
7. The QIAGEN Guide to Animal Cell Culture
8. The QIAGEN Guide to Animal Cell Culture
9. RNAi in Non-Mammalian Cultured Cells
10. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: CHO Cell Culture Application Using BioFlo Benchtop Bioreactor

(Date:7/23/2015)... -- The Hong Kong Science and Technology Parks ... of "Most Innovative Technology Hub" by The New ... innovation and technology development amongst all places in ... The award has been given by The ... and website with a large international readership. This ...
(Date:7/23/2015)... , ... July 23, 2015 , ... The Pittcon 2016 ... Harry S. Mosher Professor of Chemistry and Professor, by courtesy, of Applied Physics at ... Single-Molecule Detection in Solids Led to Super-Resolution Nanoscopy in Cells and Beyond” , Dr. ...
(Date:7/23/2015)... LONDON , July 23, 2015 ... HCM) today announces that it has signed a ... of 5,247,493 convertible preference shares held by Mitsui ... subsidiary of Chi-Med, Hutchison MediPharma Holdings Limited ("Hutchison ... Hutchison MediPharma) for 3,214,404 new ordinary shares in ...
(Date:7/23/2015)... , July 23, 2015 /PRNewswire/ – Sigma-Aldrich Corporation ... high technology company, today announced the Company has received ... its Dekalb and ... Louis . The certification extends Sigma-Aldrich,s ability to ... proteins and antibodies used in diagnostic assays, and provides ...
Breaking Biology Technology:HKSTP Wins Most Innovative Technology Hub at The New Economy Awards 2015 2HKSTP Wins Most Innovative Technology Hub at The New Economy Awards 2015 3The 2014 Nobel Prize in Chemistry Recipient W. E. Moerner, to Deliver Wallace H. Coulter Lecture at Pittcon 2016 2The 2014 Nobel Prize in Chemistry Recipient W. E. Moerner, to Deliver Wallace H. Coulter Lecture at Pittcon 2016 3Mitsui Exchanges its Shares in Hutchison MediPharma for Shares in Chi-Med 2Sigma-Aldrich Meets Evolving Quality and Regulatory Needs with ISO 13485 Expansion, Provides Diagnostic Customers with Reliable Critical Raw Materials 2Sigma-Aldrich Meets Evolving Quality and Regulatory Needs with ISO 13485 Expansion, Provides Diagnostic Customers with Reliable Critical Raw Materials 3
... 7 BioMarin Pharmaceutical Inc. (Nasdaq: BMRN ) ... Corporate Development of BioMarin, will present a company update at ... 14, 2009 at 10:40 a.m. CT. Interested parties may ... the investor section of the BioMarin website, www.BMRN.com ...
... deCODE genetics, Inc.,(Nasdaq: DCGN ) today announced that it ... release to be issued on Monday, May 11, after,the close ... a live webcast of,its conference call to discuss these results ... Daylight Time/ 12pm GMT/1pm British Summer,Time. , ...
... (NYSE: CVD ) announced that it has ... McCartney currently serves as chairman of the board of ... and supply chain solutions. He was formerly president ... multibillion dollar provider of information access technologies. He was ...
Cached Biology Technology:BioMarin to Present at the Baird Growth Stock Conference 2deCODE genetics Announces Webcast of Conference Call to Discuss First Quarter 2009 Financial Results 2Covance Elects John McCartney to Company's Board of Directors 2
(Date:6/25/2015)... 25, 2015 According to ... (Swipe & Area), Technology, Material (Optical Prism, Piezoelectric, ... Security & Others) & Geography - Global Forecast ... said market is expected to reach $14,500.07 Million ... Browse 76 market Tables and 109 ...
(Date:6/24/2015)... 2015 This report provides market analysis of ... years. It contains an analysis of the drivers, challenges, ... impact from the short, medium, and long term perspective ... market, and technology trends that currently, prevail in the ... authorities to efficiently manage the increasing marine traffic and ...
(Date:6/23/2015)... June 23, 2015   MedNet Solutions , ... the entire spectrum of clinical research, is pleased ... ™ , the company,s intuitive, flexible and affordable ... Silver 2015 Stevie® Award by the American ... Services Website category.  The American Business Awards are ...
Breaking Biology News(10 mins):Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2MedNet Solutions' iMedNet eClinical Wins Silver Stevie Award 2
... crystals measuring just a few billionths of a meter ... for a wide range of applications. Nanocrystals could be ... or the glowing pixels on high-resolution display screens. They ... The size of a semiconductor nanocrystal determines its electrical ...
... Researchers at King,s College London, in collaboration with ... have succeeded in mapping how light behaves in ... butterfly wings. Scientists have broken the limit of ... fundamental insight into how light and matter interact, ...
... ( www.medboxinc.com ) Medbox, Inc. released their ... over $820,000 for the three-month period ending June 30. Cash ... year, and receivables jumped to an impressive 1.1 million. ... and the sales of their proprietary prescription medicine dispensing systems. ...
Cached Biology News:Patterning defect-free nanocrystal films with nanometer resolution 2Patterning defect-free nanocrystal films with nanometer resolution 3Scientists shed light on glowing materials 2Scientists shed light on glowing materials 3Medbox, Inc. Releases 2nd Quarter Financials 2
Anti-human VEGFR-2/KDR (Protein-A Purified) Polyclonal Antibody...
Anti-TNKS2 Polyclonal AntibodyOther Sub-Family: not assigned-Other Applications: ELISA, Western Blot...
... (eIF4E-binding protein II) is involved in translation ... kidney. PHAS proteins bind to eIF-4E, the ... capped mRNA in vitro and in cells. ... of PHAS/eIF-4E complexes. This allows eIF-4E to ...
... (VMAT2). An antibody made to the C-terminal ... ~55 kDa in postnuclear supernatants of CHO ... type cells (2). Some additional bands, both ... detected and remain unaffected by the inclusion ...
Biology Products: