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CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

ulation. The DO loops Current Value remained higher than setpoint until the culture was introduced.
Temperature . . . . . . . . . . . . . . . .37C
pH . . . . . . . . . . . . . . . . . . . . . . . .7.2
DO . . . . . . . . . . . . . . . . . . . . . . . .50%
Agitation . . . . . . . . . . . . . . . . . . .70-130 rpm
O2 (Gases) . . . . . . . . . . . . . . . . . .4 Gas

PH Control
The pH probe was calibrated prior to the autoclave cycle (refer to BioFlo 110 Instruction Manual). CO2 gas and a liquid base were used to maintain the pH setpoint. pH control parameters were:
Base . . . . . . . . .. . .Sodium bicarbonate, 8% solution
Pump . . . . . . .. . . .Pump 4 of the 4-Pump Module
Transfer tubing . . .Narrow bore silicone tubing, as supplied (1⁄16" ID & 3⁄16" OD - NBS Catalog # P0740-2396)
Vessel inlet . . . . .. .Triport adapter in the vessel headplate

PCU setup: (pH loop)
Pump 4 plugged into "BASE" Power Controller Outlet
pH Control Selections:
Auto mode
Multiplier = 100% until base feed is started, after inoculation. Then, 25%
Multiplier = 25% after inoculation
Dead-band = 0.1
PID values: factory defaults

Dissolved Oxygen (DO) Control
The DO probe was calibrated after the autoclave cycle (refer to BioFlo 110 Instruction Manual). DO control was set to Auto mode, which automatically defaults to 4-Gas Mode after the Gas Control (O2 loop) selection is made. We also changed the P and I value of the DO loop to meet the culture demands, as shown on the next
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