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CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

onal NBS spin filter impeller for the standard pitched-blade impeller. The spin filter impeller is very useful for a continuous perfusion process because it is a simple device that lends itself to media exchange and can be easily scaled up. With most commonly available spin filters, screens rapidly become clogged when cells reach high cell density during perfusion. NBS, however, has designed spin filters with three different screen sizes that are suitable for most cell types. Feel free to contact NBS for screen size suggestions.




Control System
Listed below are six control modules included in an Advanced Cell Culture Kit, which were also used for this application:


Materials and Methods

Overview

Sterilize the vessel with phosphate buffer solution (PBS) for 60 minutes.
Remove the PBS from the vessel.
Add 1.5 L of media into the vessel.
Key in control setpoints.
Inoculate 500 mL
Use BioCommand software to automatically calculate and control glucose addition throughout the run.

Medium
EX-CELL 325 PF CHO from JRH.

Cell Line: CHO/B4C7

PCU: Select Cell Culture Application
On the initial set-up screen, the USE selector button will allow you to change the default Fermentation application to Cell Culture application. For further details refer to the Operation Section in the BioFlo 110 manual.

Control Setpoints
Setpoints were keyed into the controller prior to inoculation. The vessel was allowed to equilibrate prior to inoc
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