Listed below are six control modules included in an Advanced Cell Culture Kit, which were also used for this application:
Materials and Methods
Sterilize the vessel with phosphate buffer solution (PBS) for 60 minutes.
Remove the PBS from the vessel.
Add 1.5 L of media into the vessel.
Key in control setpoints.
Inoculate 500 mL
Use BioCommand software to automatically calculate and control glucose addition throughout the run.
EX-CELL 325 PF CHO from JRH.
Cell Line: CHO/B4C7
PCU: Select Cell Culture Application
On the initial set-up screen, the USE selector button will allow you to change the default Fermentation application to Cell Culture application. For further details refer to the Operation Section in the BioFlo 110 manual.
Setpoints were keyed into the controller prior to inoculation. The vessel was allowed to equilibrate prior to inoc