Navigation Links
CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor

onal NBS spin filter impeller for the standard pitched-blade impeller. The spin filter impeller is very useful for a continuous perfusion process because it is a simple device that lends itself to media exchange and can be easily scaled up. With most commonly available spin filters, screens rapidly become clogged when cells reach high cell density during perfusion. NBS, however, has designed spin filters with three different screen sizes that are suitable for most cell types. Feel free to contact NBS for screen size suggestions.




Control System
Listed below are six control modules included in an Advanced Cell Culture Kit, which were also used for this application:


Materials and Methods

Overview

Sterilize the vessel with phosphate buffer solution (PBS) for 60 minutes.
Remove the PBS from the vessel.
Add 1.5 L of media into the vessel.
Key in control setpoints.
Inoculate 500 mL
Use BioCommand software to automatically calculate and control glucose addition throughout the run.

Medium
EX-CELL 325 PF CHO from JRH.

Cell Line: CHO/B4C7

PCU: Select Cell Culture Application
On the initial set-up screen, the USE selector button will allow you to change the default Fermentation application to Cell Culture application. For further details refer to the Operation Section in the BioFlo 110 manual.

Control Setpoints
Setpoints were keyed into the controller prior to inoculation. The vessel was allowed to equilibrate prior to inoc
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Big Blue Rodent Cell Cultures for Mutagenesis Studies
2. Simple Isolation of RNA from Tissue and Cultured Cells
3. The QIAGEN Guide to Animal Cell Culture
4. The QIAGEN Guide to Animal Cell Culture
5. The QIAGEN Guide to Animal Cell Culture
6. The QIAGEN Guide to Animal Cell Culture
7. The QIAGEN Guide to Animal Cell Culture
8. The QIAGEN Guide to Animal Cell Culture
9. RNAi in Non-Mammalian Cultured Cells
10. Total RNA Isolation: 100 l Viral Sample From Cultured-Cell Supernatant Containing Virus
11. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: CHO Cell Culture Application Using BioFlo Benchtop Bioreactor

(Date:12/24/2014)... Dec. 24, 2014  Vermillion, Inc. (Nasdaq:   VRML), ... announced today that the Company closed its previously ... Management, Jack W. Schuler , Birchview Fund ... $10.5 million, before offering expenses.  The proceeds will ...
(Date:12/24/2014)... “Preparative & Process Chromatography Market by ... Solvents, Buffers, Valves, Guages, Seals), Accessories, Services, End ... to 2019” provides a detailed overview of the ... and strategies impacting the preparative and process chromatography ...
(Date:12/24/2014)... Boston, MA (PRWEB) December 23, 2014 Earlier ... James L. Sherley, director of the Adult Stem Cell Technology ... overlooked and under appreciated unique property of adult tissue stem ... Misunderstood in the Past, Important for the Future,” embodied the ...
(Date:12/24/2014)... Bethesda, MD (PRWEB) December 23, 2014 ... law H.R. 83, the Omnibus and Continuing Resolution Appropriations ... hydrocephalus a condition eligible to receive funding through the ... Department of Defense (DoD). The Hydrocephalus Association (HA), working ...
Breaking Biology Technology:Vermillion Announces Closing of Equity Financing 2Vermillion Announces Closing of Equity Financing 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 2Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 3Preparative and Process Chromatography Market is expected to reach $9 billion by 2019 - New Report by Marketsandmarkets 4Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4Rare Brain Condition Now Eligible for Department of Defense Research Dollars 2
... CLEVELAND, June 22, 2011 Clinical Research Management, ... in basic and applied research, clinical trials, and ... a new, 5-year, $97MM research support contract titled ... (SMMRS) to be performed at the Walter Reed ...
... June 22, 2011 ,   ... a novel platform technology for the oral delivery of ... Berelowitz, M.D., has been appointed the Chairman of its,Scientific ... Board of Directors in May 2010,and will now expand ...
... Biodesign, a drug discovery company that utilizes ultra-high throughput ... therapeutic agents against its novel disease-based targets, or its ... medicinal chemistry space at 8 Black Forest Road in ... 11,000 square feet of state-of-the-art research laboratories and office ...
Cached Biology Technology:ClinicalRM (formerly CRM) Awarded $97MM U.S. Army Contract to Support Military Clinical Research for the Warfighter 2Oramed Pharmaceuticals Appoints Dr. Michael Berelowitz as Chairman of Scientific Advisory Board 2Oramed Pharmaceuticals Appoints Dr. Michael Berelowitz as Chairman of Scientific Advisory Board 3VENENUM Biodesign Announces Opening of Medicinal Chemistry Labs 2
(Date:12/17/2014)... Dec. 15, 2014  HITLAB SM announced today its ... confirm its adherence to current U.S. Food and Drug ... regulated smart device and smart phone application trials utilizing ... "HITLAB is determined to improve global healthcare ...
(Date:12/11/2014)... , Dec. 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/twhjll/biometrics_market ) has announced the addition of the ... to their offering. One major trend ... continuous advances in technology, it is important to upgrade ...
(Date:12/10/2014)...  Valencell, a leader in performance biometric data sensor technology, ... its licensees for highly accurate, clinically validated biometric technology. ... from fitness and health sectors, but first responders/military and ... only as useful as the biometric data it delivers. ...
Breaking Biology News(10 mins):HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2Biometrics Market in the APAC Region 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2Wearable Technology Products Demand Highly Accurate Biometric Technology 2
... to accurately measure the levels of important chemicals in ... level of a single cell. Scientists at the Carnegie ... the first to overcome this obstacle by successfully applying ... brain chemical levels. The sensors alter their 3-dimensional form ...
... A research team based at Massachusetts General Hospital (MGH) ... of RNA interference (RNAi), a powerful new research tool ... the RNAi process itself to find new genes that ... to fight human disease. The report will appear in ...
... Montreal is pleased to announce the first successful birth in ... Seang Lin Tan, Chair of the Department of Obstetrics and ... Centre at the MUHC in Montreal confirmed the birth of ... are the first in-vitro fertilization (IVF) Centre in Canada to ...
Cached Biology News:Revolutionary nanotechnology illuminates brain cells at work 2Revolutionary nanotechnology illuminates brain cells at work 3Scientists identify genetic pathways essential to RNA interference 2Scientists identify genetic pathways essential to RNA interference 3First frozen egg baby born in Canada 2First frozen egg baby born in Canada 3
... solution, Type I is ... and agarose gel electrophoresis ... offers the advantage of ... blue and xylene cyanole). ...
Rabbit polyclonal to QKI...
... SilverSNAP Stain for Mass Spectrometry Applications ... spectrometry (MS) has emerged as a powerful ... of 2-D gels is now an important ... that leads ultimately to identification of specific ...
... without ammonium sulfate are compatible with thermostable ... Taq and AmpliThem™™ DNA polymerases. You can ... in one experiment by adding your own ... PreMixes supplied with the kit. This capability ...
Biology Products: