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CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor


Introduction
This Application Report is one in a series documenting simple protocols for culturing cells in a BioFlo 110 benchtop fermentor/bioreactor system. The modular BioFlo 110 is offered with an extensive choice of vessels, impellers and control modules to enable growth of a wide variety of cultures, including mammalian, insect, plant, yeast and bacterial cells. Complete kits are also available. This report concentrates on achieving high-density CHO cell cultures using NBS Advanced Cell Culture Kit.

For this application, researchers in NBS cell culture laboratory used a:
BioFlo 110 Advanced Cell Culture Kit with a magnetic drive and a 3.0L total volume water-jacketed vessel, (nominal working volume 2.2L.) (NBS Catalog # M1273-1212)
Suspension Cell Spin Filter with a 12-14 micron screen (NBS Catalog # M1273-3202) for continuous perfusion culture
BioCommand Plus Supervisory Software (NBS Catalog # M1291-0000) to control the feeding schedule and collect data
NucleoCounter Automated Cell Counting System (NBS Catalog # M1293-0000) to count viable cells.


The Bioreactor
All BioFlo 110 Advanced Cell Culture Kits are configured with six control modules, a complete vessel assembly including a pitched blade agitation impeller, foam/level sensors, as well as dissolved oxygen and pH probes (Mettler Toledo), three liquid addition bottles, cables, tubing, clamps and other related items. Comes with choice of direct drive or magnetic-drive agitation systems, as well choice of water-jacketed or heat-blanketed vessel options.

For this application we substituted an opti
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