Navigation Links
C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

Polymerase for two-step RT-PCR amplification of up to 4 kb targets

Cat. No. 2 016 311 300 units

Description C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. Application Produces first strand cDNA (for RT-PCR) or full-length double-stranded cDNA transcripts from total RNA or mRNA. May be used for:
  • Two-step RT-PCR for virus detection, cloning, mutation analysis, etc.
  • One-step RT-PCR (e.g. when used with Taq DNA Polymerase).
  • One-Step RT-PCR System.
  • RT-PCR amplification of GC-rich sequences.
Operating parameters
  • Temperature optimum, reverse transcriptase reaction: 5572C.
  • Divalent ion requirement: Mg2+.
  • Addition of betaine (0.52.0 M) to reaction mix improves yield and specificity of transcription for GC-rich templates.
  • Accepts DIG-labeled and other modified nucleotides.
  • Works with Uracil-DNA Glycosylase (PCR carry-over prevention).
Key advantages
  • Enhances specificity, because the high reaction temperature reduces nonspecific primer binding.
  • Ensures high fidelity first strand cDNA, because the enzyme has a twofold lower transcriptional error rate than Tth DNA Polymerase.
  • Efficiently transcribes GC-rich mRN
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. C. therm. Polymerase One-Step RT-PCR System
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Pfu DNA Polymerase as a Hot Start Alternative
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. High-Fidelity PCR with a Novel Polymerase Mixture
9. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
10. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
11. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: therm Polymerase for Reverse Transcription Two Step PCR

(Date:8/20/2014)... (PRWEB) August 20, 2014 Not long ... E&L studies should be performed late in the development ... known. But current regulatory trends suggest that, like many ... the rise. In recent years, multiple drug sponsors have ... or address specific questions during phase I/II. It’s not ...
(Date:8/20/2014)... Stimulating nerves in your ear could improve the health ... at the University of Leeds used a standard TENS ... apply electrical pulses to the tragus, the small raised ... front of the ear canal. , The stimulation changed ... by reducing the nervous signals that can drive failing ...
(Date:8/20/2014)... Sofia, Bulgaria (PRWEB) August 20, 2014 ... was released today including improvements to the ... rates and connectors for Lucene, SOLR and Elasticsearch. ... change for this high performance triplestore – GraphDB™ ... organizations interested in deploying the only mature enterprise ...
(Date:8/19/2014)... August 19, 2014 Shimadzu Scientific ... systems, Prominence-i and Nexera-i, adding to the company’s ... excellent functionality, an intuitive operating environment, and full ... more efficient workflow for conventional to ultra-high-speed analysis. ... intuitive and intelligent design so users can begin ...
Breaking Biology Technology:Are Extractable and Leachables going Phase-Appropriate? New Webinar Hosted by Xtalks 2'Tickling' your ear could be good for your heart 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 2Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 3Ontotext Improves Its RDF Triplestore, GraphDB™ 6.0: Enterprise Resilience, Faster Loading Speeds and Connectors to Full-Text Search Engines Top the List of Enhancements 4Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 2Shimadzu’s New i-Series Integrated Liquid Chromatography Systems Provide Laboratories Wider Range of Analytical Capabilities 3
... ever involving patients with cutaneous T-cell lymphoma on schedule ... RADNOR, Pa., June 29 Yaupon Therapeutics, a privately ... enrollment for a pivotal Phase 2 clinical trial for ... (CTCL - stages 1-2a). The study, which is ...
... DOR BioPharma, Inc. ... late-stage biopharmaceutical company, announced today that investigators at the ... paper this morning in Bone Marrow Transplantation ... (oral beclomethasone dipropionate or BDP) decreases non-infectious inflammation of ...
... 29 /PRNewswire-FirstCall/ - YM BioSciences Inc. (NYSE Amex: ... product development company that identifies and advances a ... stages of development, today reported that YM,s subsidiary, ... the issues between the companies in a cooperative ...
Cached Biology Technology:Yaupon Therapeutics Completes Patient Enrollment for Pivotal Phase 2 Study of Clearazide for Treatment of Cutaneous T-cell Lymphoma 2DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 2DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 3DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 4DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 5DOR BioPharma Announces Publication of orBec(R) Clinical Pulmonary Data in Bone Marrow Transplantation 6YM BIOSCIENCES' SUBSIDIARY RESOLVES PROPOSED ARBITRATION WITH ONCOSCIENCE AG 2YM BIOSCIENCES' SUBSIDIARY RESOLVES PROPOSED ARBITRATION WITH ONCOSCIENCE AG 3YM BIOSCIENCES' SUBSIDIARY RESOLVES PROPOSED ARBITRATION WITH ONCOSCIENCE AG 4
(Date:8/21/2014)... implications for life in other extreme environments, both on ... Associate Professor of Biological Sciences Brent Christner and fellow ... this week published a paper confirming that the waters ... (2600 feet) beneath the surface of the West Antarctic ... than 400 subglacial lakes and numerous rivers and streams ...
(Date:8/21/2014)... 18-22, 2014 , WHERE: , San Diego ... CA 92101 , WHAT: , Invited and ... latest research in human genetics. Examples of sessions ... on rare genetic variants in health and disease, ... sun sensitivity (Saturday, Oct. 18, 5:30-7:30 pm) , ...
(Date:8/21/2014)... researchers at Louisiana Tech University has developed ... 3D printers and materials to fabricate custom ... chemotherapeutic compounds for targeted drug delivery. , ... research faculty from Louisiana Tech,s biomedical engineering ... filament extruders that can make medical-quality 3D ...
Breaking Biology News(10 mins):800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 2800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 3800 meters beneath Antarctic ice sheet, subglacial lake holds viable microbial ecosystems 4American Society of Human Genetics 2014 Annual Meeting 2Louisiana Tech University researchers use 3D printers to create custom medical implants 2Louisiana Tech University researchers use 3D printers to create custom medical implants 3
... Hopkins have discovered that a tiny piece of genetic code ... and it gets there under its own internal code. , ... that directs its movement, will be published Jan. 5 in ... development, latch on to and gum up larger strands of ...
... risk factor for Alzheimer's disease puts out the welcome mat for ... more active in the brain compared to other forms of the ... Aging, add some scientific heft to the idea, long suspected by ... Alzheimer's disease. , The work links a form of the ApoE ...
... percent of men treated with a combination of radiation ... cancer are cured of their disease 15 years following ... the International Journal of Radiation Oncology*Biology*Physics, the official journal ... physicians at the Seattle Prostate Institute. Doctors wanted to ...
Cached Biology News:Renegade RNA -- Clues to cancer and normal growth 2Cold sore virus might play role in Alzheimer's disease 2Cold sore virus might play role in Alzheimer's disease 3Cold sore virus might play role in Alzheimer's disease 4
... The Nick Translation System is ideal ... DNA. The Nick Translation System: Provides ... labeled nucleotide incorporation Yields >10 8 ... Includes control DNA to monitor the performance ...
... portable, easy-to-use and makes identifying your samples more ... to use Labels withstand extreme lab ... Time/Date stamp Laboratory symbols ... Serialization feature makes you more efficient ...
... Direct SPA Screening Assay, 5 ... that eliminate lengthy sample extraction procedures ... use).Range: 1.32-84.28 ng/ml (4-256 pmol/ml).Sensitivity: 0.65 ... 2-8 C. Category: Drug Screening & ...
The E. coli Expression System with Gateway Technology is designed to create E. coli expression clones containing the T7 promoter. The expression clones are ready for transformation and expression in ...
Biology Products: