Navigation Links
C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

Polymerase for two-step RT-PCR amplification of up to 4 kb targets

Cat. No. 2 016 311 300 units

Description C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. Application Produces first strand cDNA (for RT-PCR) or full-length double-stranded cDNA transcripts from total RNA or mRNA. May be used for:
  • Two-step RT-PCR for virus detection, cloning, mutation analysis, etc.
  • One-step RT-PCR (e.g. when used with Taq DNA Polymerase).
  • One-Step RT-PCR System.
  • RT-PCR amplification of GC-rich sequences.
Operating parameters
  • Temperature optimum, reverse transcriptase reaction: 5572C.
  • Divalent ion requirement: Mg2+.
  • Addition of betaine (0.52.0 M) to reaction mix improves yield and specificity of transcription for GC-rich templates.
  • Accepts DIG-labeled and other modified nucleotides.
  • Works with Uracil-DNA Glycosylase (PCR carry-over prevention).
Key advantages
  • Enhances specificity, because the high reaction temperature reduces nonspecific primer binding.
  • Ensures high fidelity first strand cDNA, because the enzyme has a twofold lower transcriptional error rate than Tth DNA Polymerase.
  • Efficiently transcribes GC-rich mRN A regions, because the thermoactive enzyme can operate at temperatures (up to 72C) high enough to eliminate most secondary structure, even in GC-rich regions.
  • Accurately transcribes even low amounts of template (high sensitivity).
  • Flexible, because the enzyme can use either oligo(dT) primer or anchor primer at temperatures up to 60C.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. C. therm. Polymerase One-Step RT-PCR System
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Pfu DNA Polymerase as a Hot Start Alternative
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. High-Fidelity PCR with a Novel Polymerase Mixture
9. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
10. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
11. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:7/20/2017)... ... July 20, 2017 , ... Crucial Data Solutions ... and study participants truly unified. TrialKit, a native mobile app, empowers investigators and ... research studies entirely on mobile devices. With TrialKit, clinical researchers can utilize Core ...
(Date:7/18/2017)... (PRWEB) , ... July 18, 2017 , ... ... today announced that Merck, a leading science and technology company, has implemented ... therapeutics for the therapeutic areas of Oncology, Immunology, and Neurodegenerative Diseases. , The ...
(Date:7/18/2017)... ... July 18, 2017 , ... Allotrope Foundation won the ... phase of the Allotrope Framework for commercial use. , The Bio-IT World Best ... only elevate the critical role of information technology in modern biomedical research, but ...
(Date:7/17/2017)... ... July 17, 2017 , ... The Academy of Model Aeronautics (AMA) will ... Aviation Day will take place from 9 a.m. to 2 p.m. at the International ... all ages. , Aviation Adventure Day will be packed with entertaining activities for the ...
Breaking Biology Technology:
(Date:3/30/2017)... , March 30, 2017 Trends, opportunities and ... and behavioral), by technology (fingerprint, AFIS, iris recognition, facial ... and others), by end use industry (government and law ... financial and banking, and others), and by region ( ... , Asia Pacific , and the ...
(Date:3/28/2017)... -- The report "Video Surveillance Market by ... Devices), Software (Video Analytics, VMS), and Service (VSaaS, Installation ... 2022", published by MarketsandMarkets, the market was valued at ... reach USD 75.64 Billion by 2022, at a CAGR ... considered for the study is 2016 and the forecast ...
(Date:3/24/2017)... MILAN , March 24, 2017 The Controller ... Deputy Controller Mr. Abdulla Algeen have received the prestigious international ... Continue Reading ... ... small picture) and Deputy Controller Abdulla Algeen (small picture on the right) ...
Breaking Biology News(10 mins):