Navigation Links
C. therm. Polymerase for Reverse Transcription in Two-Step RT-PCR

Polymerase for two-step RT-PCR amplification of up to 4 kb targets

Cat. No. 2 016 311 300 units

Description C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. Application Produces first strand cDNA (for RT-PCR) or full-length double-stranded cDNA transcripts from total RNA or mRNA. May be used for:
  • Two-step RT-PCR for virus detection, cloning, mutation analysis, etc.
  • One-step RT-PCR (e.g. when used with Taq DNA Polymerase).
  • One-Step RT-PCR System.
  • RT-PCR amplification of GC-rich sequences.
Operating parameters
  • Temperature optimum, reverse transcriptase reaction: 5572C.
  • Divalent ion requirement: Mg2+.
  • Addition of betaine (0.52.0 M) to reaction mix improves yield and specificity of transcription for GC-rich templates.
  • Accepts DIG-labeled and other modified nucleotides.
  • Works with Uracil-DNA Glycosylase (PCR carry-over prevention).
Key advantages
  • Enhances specificity, because the high reaction temperature reduces nonspecific primer binding.
  • Ensures high fidelity first strand cDNA, because the enzyme has a twofold lower transcriptional error rate than Tth DNA Polymerase.
  • Efficiently transcribes GC-rich mRN A regions, because the thermoactive enzyme can operate at temperatures (up to 72C) high enough to eliminate most secondary structure, even in GC-rich regions.
  • Accurately transcribes even low amounts of template (high sensitivity).
  • Flexible, because the enzyme can use either oligo(dT) primer or anchor primer at temperatures up to 60C.


'"/>

Source:


Page: All 1 2

Related biology technology :

1. C. therm. Polymerase One-Step RT-PCR System
2. Novel PCR Enhancing Factor Improves Performance of Pfu DNA Polymerase
3. Pfu DNA Polymerase as a Hot Start Alternative
4. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
5. pfuturbo DNA Polymerase: A High-Performance, High-Fidelity Enzyme Ideal for PCR Cloning
6. Unique DNA Polymerase Formulation Excels in a Broad Range of PCR Applications
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. High-Fidelity PCR with a Novel Polymerase Mixture
9. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
10. Unique Enhanced DNA Polymerase Delivers High Fidelity and Great PCR Performance
11. The Use of MasterTaq Polymerase for PCR with Humic Material-Contaminated DNA
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:1/18/2017)... Texas , Jan. 18, 2017  Caris ... and the Lustgarten Foundation, the largest private funder ... a clinical trial evaluating the impact of immunotherapy ... providing clinical trial enrollment services to identify potential ... facilitate communication between treating physicians and study investigators. ...
(Date:1/17/2017)... Jan. 17, 2017 The Global Implantable ... CAGR of around 7.5% over the next decade ... of the prominent trends that the market is ... diseases & graft transplant surgeries and medical implants ... market is categorized into immunomodulatory biomaterials, natural, polymers, ...
(Date:1/17/2017)... NEW YORK , Jan. 17, 2017 /PRNewswire/ ... mobile health coaching, is the first to offer ... and obesity. Noom,s Spanish diabetes prevention ... expand the reach and accessibility of lifestyle interventions ... of developing these conditions. Noom,s robust food database, ...
(Date:1/17/2017)... ... ... Pono Ola , a mind-body wellness firm on a mission to ... of its much-anticipated Pono Board: a re-invented fitness and anti-fatigue balance board that engages ... year, the patented Pono Board is the world’s only exercise balance board built to ...
Breaking Biology Technology:
(Date:1/6/2017)... Jan. 6, 2017  Privately-held CalciMedica, Inc., announced ... healthy volunteers of a novel calcium release-activated calcium ... pancreatitis. Acute pancreatitis, sudden painful ... disorder, but can be very serious.  In severe cases ... where extended hospital stays, time in the ICU ...
(Date:1/4/2017)... LAS VEGAS , Jan. 4, 2017 /PRNewswire/ ... in performance biometric sensor technology, today announced the ... Benchmark™ sensor systems, the highly-accurate biometric sensor ... ® biometric technology, experience and expertise. The ... of Benchmark designed specifically for hearables, and Benchmark ...
(Date:12/20/2016)... 2016 The rising popularity of mobility ... is stoking significant interest in keyless access systems. ... low energy (BLE), biometrics and near-field communication (NFC) ... of wireless technologies in the automotive industry. This ... access systems opens the market to specialist companies ...
Breaking Biology News(10 mins):