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C. therm. Polymerase One-Step RT-PCR System

for one-step RT-PCR amplification of up to 3 kb RNA targets Cat. No. 2 016 338 50 reactions
Cat. No. 2 016 346 250 reactions Description The system contains a unique enzyme blend of C. therm. Polymerase and Taq DNA Polymerase.

Note: C. therm. Polymerase provided by Roche Applied Science is the recombinant form of the DNA polymerase from the thermophilic eubacterium Carboxydothermus hydrogenoformans, expressed in E. coli. The recombinant enzyme lacks the 5'3' exonuclease domain (RNase H activity) of the native enzyme. The system also contains RT-PCR reaction buffer (5x conc.) with 12.5 mM MgCl2 and 10% DMSO; DMSO solution; and 100 mM DTT solution. Application The C. therm. Polymerase One-Step RT-PCR System may be used for a one-tube, one-step RT-PCR. The C. hydrogenoformans DNA polymerase produces first strand cDNA with high yield and specificity, then Taq DNA Polymerase amplifies the cDNA. The system may be used for:
  • Amplification of up to 3 kb targets (especially GC-rich sequences) from mRNA or total RNA for virus detection, cloning, mutation analysis, etc.
  • Qualitative, semi-quantitative, or quantitative analysis of RNA transcription levels (in combination with gel-based or ELISA detection methods).
  • RT-PCR in the presence of dUTP for prevention of carry-over contamination in combination with UNG (see the pack insert for details).
Application profile Operating paramet
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