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Brucella abortus

Trypticase soy broth at 37 C with vigorous shaking, chill on ice for 10 min.
  • Harvest by centrifugation (10 min, 10,000 x g). Wash three times in an equal volume of sterile cold water.
  • Resuspend cells in 1/500 volume of 10% glycerol (number of cells: 1010 cells/ml). Freeze aliquots on dry ice and
    store at 70 C.

    Mode Prokaryotes O Voltage (V) 2,500 V Time constant (T) 5 ms
  • Immediately add 1 ml trypticase soy broth, incubate 6 hours at 37 C.
  • Plate cells on selective tryptose agar plates, incubate at 37 C for four days.


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