( ...)Brucella abortus

Multiporator / Electroporator 2510 Transformation Protocol Protocol No. 4308 915.508 12/2001 Microorganism Brucella abortus Cell type Bacteria, gram negative Molecules injected Plasmid DNA (pBA.sodkn^r) Growth medium Trypticase soy broth Washing solution Sterile, cold water Electroporation solution 10% glycerol Outgrowth medium Trypticase soy broth Cuvette 2 mm gap width Reference Tatum F.M., et al 1992 Infection and Immunity 60 2863-2869

Making electrocompetent cells:

1. Grow cells at 30 C to a density of O.D.₆₀₀: 1.5-2.0.


2. Harvest by centrifugation and wash five to seven times in sterile, cold water.


3. Resuspend the cells in 10% glycerol to a final concentration of 4-6 x 10¹¹ cell/ml and store frozen at 70 C.


4. For electroporation frozen cells were thawed in ice and diluted with sterile water to a density of 1 x 10¹⁰ cells/ml prior to use.

Electroporation of cells:

1. Grow cells in
   '"/>

Source:


Page: All 1 2
Related biology technology :

1. Brucella abortus