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Brinkmann/Eppendorf SOP,,, Standard Operating Procedure for Pipettes

Balance tested by Board of Weights and Measures (NIST) (e.g. Sartorius, Mettler, Ohaus or AnD)
Certified Barometer, Thermometer, and Hygrometer

Resolution/Accuracy:
The relationship between the error limits of the pipettes to be tested and the graduation scale of the balance ensures that scattering within a measurement series is recorded with sufficient accuracy to evaluate the accuracy and precision in accordance with the DIN standard. Manufacturer specifications sometimes deviate from the DIN standard and demand a high degree of weighing accuracy (see Technical specifications).

Nominal volume of the pipette
to be tested (l)
Error limits of the device
to be tested (l)
Required accuracy
of balance (g)
1 50* 0.1 0.8 0.00001 > 50 1,000 1.0 10 0.0001 > 1,000 > 10 0.001 * A balance with six decimal places or, where appropriate, a photometric test should be used for volumes <= 1 l. Evaporation protection:
Moisture trap or other equipment which prevents evaporation.

Electrostatic charge:
For test volumes <= 50 l, electrostatic discharge to all parts which come in direct or indirect contact with the pipettes must be prevented. The tubes provided by Eppendorf and the moisture trap with lid are made of glass, which minimizes electrostatic charge.

Measuring station:
Calibration s
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Source:


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