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Brinkmann/Eppendorf SOP,,, Standard Operating Procedure for Pipettes

- Have Eppendorf Pipette Tips been attached (pipette + pipette tip -> pipetting system)?
Volume errors may occur during the pipetting of liquids which have a high vapor pressure or a density which is significantly different to that of water.
Do not adjust the device until all of these points have been examined. 8.1 Adjusting pipettes (adjustable-volume)

The adjustment of adjustable-volume pipettes Series 2100 (Research), Model 4810, Series 2000 (Reference)
adjustable-volume, Positive Displacement Pipette (Model 4830),
and Titermate is a zero-point shift.
  1. In accordance with the Standards, Part 6; the temperature of the device, the Eppendorf Pipette Tip, the test liquid, and the ambient conditions must be the same (20 25 C constant to 0.5 C) during testing.
  2. Set the device to the smallest possible volume, which should not be less than 10% of the nominal volume.
  3. When using single-channel pipettes, attach an appropriate Eppendorf Pipette Tip to the pipette. With multichannel pipettes, the tip can be attached to any one channel.
  4. Pipette the volume ten times. The actual volume is calculated from the mean value obtained from these weighings (use the Z factor to convert mg into l).
  5. To adjust the device, insert the tool provided into the appropriate opening and set the actual volume (see Fig. 1 8 or consult the instruction manual).
  6. Check the volume which has been set by carrying out a measurement once again. If the nominal volume does not correspon
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