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Brinkmann/Eppendorf SOP,,, Standard Operating Procedure for Pipettes

as described above. Evaluate the inaccuracy and the imprecision - With adjustable pipettes, proceed in the same way, using the nominal volume and 50% of the nominal volume - With multichannel pipettes, the measured values of all channels per test volume are summarized for evaluation of the inaccuracy and imprecision Please note: When user testing, follow section 3.1 Full Certification Procedure.

4 Individual evaluation

The following calculation is carried out from all measured values within the evaluation limits: Mean value = x Number of measured values = n Standard deviation = s Coefficient of variation = CV (imprecision) Inaccuracy = d Range = Difference between maximum and minimum value For exact calculation, Z factor is used to correct changes in the test liquid regarding temperature and air pressure.

The manufacturer specifications for the pipette can be found in the instruction manual or in Technical specifications

5 Sterilizing and cleaning the pipette

Sterilization
The instruction manual of the pipette contains information on cleaning with solvents or autoclaving(121 C, 20 min.).


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