Navigation Links
Brilliant Core Reagent Kits for QPCR and QRT-PCR

b> (Figure 2). This large dynamic range eliminated the need to analyze serial dilutions of experimental samples for gene quantification. Moreover, this target titration demonstrated that the Brilliant Plus QPCR kit is sensitive enough to detect as few as 10 copies of plasmid DNA.

To test the compatibility of the Brilliant Plus QPCR core reagent kit with different fluorescent chemistries, we used a Scorpions probe to detect and amplify the 134-bp rpoB gene target of Mycobacterium tuberculosis, an emergent pathogen. We amplified and detected 100, 1000, and 10,000 copies of the M. tuberculosis target gene; the respective Ct values were 38.2, 34.9, and 31.6 (data not shown).

Multiplex Capabilities

Fig.3

In further experiments, we showed that the Brilliant Plus QPCR reagent kits can efficiently detect more than one dye per reaction (Multiplex QPCR or MxQPCR). We used Stratagenes molecular beacon SDF-1 (Stromal Derived Factor 1) Allelic Discrimination kit, which detects a common G-to-A substitution that may delay or accelerate the onset of AIDS in humans (Figure 3). We detected this substitution by amplifying human genomic DNA in the presence of two molecular beacons in the same tube, each molecular beacon with a different dye (FAM and TET). In this scheme, the FAM probe was complementary to the mutant allele (A), and the TET probe was complementary to the wild-type allele (G). We included the passive reference dye, although not required, as the third fluorophore. The genotype-specific control DNA samples supplied in the molecular beacon SDF-1 kit were used for this allelic discrimination.

This multiplex assay provides a way of determining whether a
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
3. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
4. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
5. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
6. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
7. TransMessenger Transfection Reagent
8. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Effectene Transfection Reagent
11. PolyFect Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Brilliant Core Reagent Kits for QPCR and QRT PCR

(Date:5/4/2015)... ROCKVILLE, Md. , May 4, 2015 /PRNewswire/ ... "RegeneRx") today announced that an independent team of ... have reported that Thymosin B4 (TB4) plays an ... dermal burn wounds, induced in an experimental mouse ... rapid vascular remodeling of damaged dermal tissue.  The ...
(Date:5/4/2015)... , May 4, 2015  Soligenix, Inc. (OTCQB: ... developing products that address unmet medical needs in the ... it will be presenting preclinical data from three of ... Defense Science and Technology Conference on May 12-14, 2015 ... at 701 Convention Plaza, St. Louis MO ...
(Date:5/4/2015)... May 4, 2015   Tocagen Inc. , ... data from Tocagen,s ongoing investigational studies were presented ... the American Association of Neurological Surgeons (AANS)/Congress of ... 1-2, 2015, in Washington, D.C. ... a meeting with FDA and continues to advance ...
(Date:5/4/2015)... Marianjoy Rehabilitation Hospital ... and hand impairments by acquiring the Armeo®Spring arm ... network site. , Secured through grant support of ... treatment option for patients with upper extremity weakness ... one of just four Illinois providers offering treatment ...
Breaking Biology Technology:Researchers Report that Thymosin B4 has a Major Role in Dermal Burn Wound Healing 2Researchers Report that Thymosin B4 has a Major Role in Dermal Burn Wound Healing 3Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 2Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 3Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 4Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 5Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 6Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 7Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 8Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 9Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 10Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 11Soligenix Announces Presentation and Poster at the 2015 Chemical and Biological Defense Science and Technology Conference in St. Louis, MO 12Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 2Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 3Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 4Tocagen Presents Updated Interim Survival Data from Ongoing Clinical Studies at American Association of Neurological Surgeons (AANS)/Congress of Neurological Surgeons (CNS) Section on Tumors 5Marianjoy Acquires Armeo®Spring Arm and Hand Rehabilitation Exoskeleton at Oakbrook Terrace Facility 2
... Genoptix, Inc.,(Nasdaq: GXDX ), a specialized laboratory services ... Life Sciences Conference,scheduled to take place at the Grand ... 2008., Douglas Schuling, Senior Vice President and CFO ... 22, beginning at,approximately 3:30 pm EDT. Mr. Schuling will ...
... of oil palm plantations will worsen the dual environmental ... are better protected, warn scientists in the most comprehensive ... Emily Fitzherbert from the Zoological Society of London and ... debate over the role of palm oil production in ...
... will likely be the move from today,s two-dimensional chips ... is now running at 1.4 gigahertz at the University ... Rochester chip is not simply a number of regular ... designed and built specifically to optimize all key processing ...
Cached Biology Technology:Genoptix Announces Participation at the UBS Global Life Sciences Conference 2Oil palm plantations are no substitute for tropical rainforests, a new study shows 2Oil palm plantations are no substitute for tropical rainforests, a new study shows 3First 3-D processor runs at 1.4 Ghz on new architecture 2
(Date:4/27/2015)... , Apr. 27, 2015 NXT-ID, Inc. ... biometric authentication company focused on the growing mobile commerce ... shipping to pre-order customers the first week of May, ... the month of May. Gino ... significant milestone for the company as Wocket® enters the ...
(Date:4/21/2015)... BURNABY, Canada , April 21, 2015 ... spending and evolving government policies are boosting access control ...   According to a recently published report ... & Opportunities, 2020 ", the access control systems market ... to nearly USD1.2 billion by 2020.The access control systems ...
(Date:4/20/2015)... April 20, 2015 The announcement ... Glenbeigh Records Management (GRM), Ireland,s ... Islamic Bank. Having built up an impressive track record of ... leading player within the records management sector in ... double is GCC staffbase and employ a further eight staff ...
Breaking Biology News(10 mins):Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 2Wocket, the Smartest Wallet You Will Ever Own, Announces Shipment to Pre-order Customers 3Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 2Saudi Arabia Access Control Systems Market to Touch USD1.2 Billion by 2020 Says TechSci Research 3Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 2Glenbeigh Records Management Wins Contract With Dubai Islamic Bank 3
... oyster native to the West Coast could face a double ... research from the University of California, Davis, Bodega Marine Laboratory. ... Proceedings of the Royal Society B . Invasive snails ... snails were raised under ocean conditions forecast for the end ...
... Minn. , Jan. 15, 2014 MedNet Solutions ... study management systems, is pleased to announce that the latest ... released to the iMedNet user community.  iMedNet ... eClinical platform ideally suited to Sponsors and CROs wanting to ...
... news release is available in German . ... economists and politicians usually call a win-win situation: a relationship between ... acacia plants and the ants that live on them is an ... of food bodies and nectar as well as hollow thorns which ...
Cached Biology News:Acidification, predators pose double threat to oysters 2MedNet Solutions Announces Latest Release Of Its Innovative iMedNet EDC eClinical Solution 2Ants protect acacia plants against pathogens 2Ants protect acacia plants against pathogens 3
CASPR (H-66)...
Homo sapiens toll interacting protein Antigen: Recombinant Protein...
Human SIGIRR MAb (Clone 162201)...
... EPS 1001 Power Supply, 1. 1000 ... voltage, constant current, and constant power ... for precision and reproducibility.Stores and recalls ... flatbed gels on Multiphor II ...
Biology Products: