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Brilliant Core Reagent Kits for QPCR and QRT-PCR

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AmpliTaq Gold and MultiScribe RT

dUTP only for single-tube kit

In Buffer

200

$700

$3.50

#N808-0232

Platinum Quantitative RT-PCR ThermoScript
One-Step System

Platinum Taq ThermoScript

No

No

500

$1,575

$3.00

#11721023

Available in either a single- or two-step format (for those who wish to archive a portion of the RT reaction prior to PCR), the Brilliant and Brilliant Plus QRT-PCR kits include high-performance StrataScript RNaseH-minus reverse transcriptase and SureStart Taq DNA polymerase, which has a hot-start feature. For compatibility with carry-over contamination-control strategies, the Brilliant Plus kits also include dUTP and uracil-N-glycosylase (UNG) for decontamination of amplicons. (The Single-Step RT-PCR Kit contains dUTP only.) While the dUTP provides a convenient decontamination strategy, incorporation of dUTP is slightly less efficient than dTTP and the Brilliant Plus kits are slightly less sensitive than the standard Brilliant kits.

By including a passive reference dye as a separate component, reaction volume pipeting error can be normalized from well to well. Users have the option of including or not including the passive reference dye in their reactions. While a passive reference dye is very helpful, it does consume some of the useable light spectrum and thus reduces the number of different dyes that can be used in muliplex reactio
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