Navigation Links
Brilliant Core Reagent Kits for QPCR and QRT-PCR

-template control did not show any amplification (Figure 4).

To evaluate performance, we compared Stratagenes Brilliant single-step QRT-PCR kit to a another commercially available quantitative RT-PCR one-step system by amplifying and detecting a 150-bp region of human GAPDH mRNA using the Molecular Beacon GAPDH Expression Analysis kit. For this comparison, we amplified three concentrations (100, 1, and 0.01 ng) of human total RNA (Methods); however, because the other commercially available kit did not provide a passive reference dye, none was included in this experiment. We found that for each concentration of target RNA, the Ct was comparable or better for the Brilliant kit (data not shown). We assessed the sensitivity of the Brilliant two-step QRT-PCR kit to detect a low-abundance target by amplifying and detecting hypoxanthine phosphoribosyl transferase (HPRT) mRNA from human brain total RNA. For the real-time analysis of the RT-PCR amplification, we used the primers and linear hydrolysis probe from the TaqMan pre-developed assay reagents (PE Biosystems) in the Brilliant Plus two-step QRT-PCR kit. We included UNG and the passive reference dye.The following Ct values were obtained: 22.5 (100 ng), 26.4 (10 ng), 29.1 (1 ng), 32.7 (100 pg), and 37.7 (10 pg) (data not shown). The results show that with the Brilliant Plus two-step quantitative RT-PCR kit, a low-abundance mRNA can be detected from as little as 10 pg of total RNA.

Conclusions

The Brilliant QPCR and QRT-PCR core reagent kits provide highly sensitive and versatile quantitative assessments for real-time gene quantification and gene expression analysis. The kits are compatible with linear hydrolysis (TaqMan probes), molecular beacon, single dye primers, and Scorpions fluorescent technologies. With the Brilliant QPCR core reagent kit, we detected as few as 10 copies of mouse muscle nicotinic ace
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
3. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
4. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
5. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
6. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
7. TransMessenger Transfection Reagent
8. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Effectene Transfection Reagent
11. PolyFect Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Brilliant Core Reagent Kits for QPCR and QRT PCR

(Date:7/10/2014)... Calif., July 10, 2014   Ceres, Inc . ... seed company, announced today financial results for the three ... on its business. Ceres reported that the ... product performance this growing season in Brazil, which concluded ... the company,s sorghum evaluation areas for part of the ...
(Date:7/10/2014)... Using microscopic polymer light resonators that expand in ... Quantum Photonics Laboratory have developed new optical sensors ... Optical sensors are ideal for detecting trace gas ... lightweight nature, and immunity to electromagnetic interference. , ... before, the MIT team conceived an extremely sensitive, ...
(Date:7/10/2014)... Columbia researchers have observed the fractional quantum Hall effect ... of matter can be tuned by an electric field. ... occur when electrons confined to thin sheets are exposed ... collective behavior where thousands of individual electrons behave as ... this effect is well established, many details of this ...
(Date:7/10/2014)... 1960s, theatergoers have shelled out for crude 3-D ... their viewing experience. These basic devices, used to ... reality, may soon be rendered obsolete with the ... Aviv University researchers. , TAU doctoral students Yuval ... highly efficient holography based on nanoantennas that could ...
Breaking Biology Technology:Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9Swell new sensors 2Columbia researchers observe tunable quantum behavior in bilayer graphene 2Columbia researchers observe tunable quantum behavior in bilayer graphene 3Projecting a 3-dimensional future 2Projecting a 3-dimensional future 3
... simultaneously with the tempest thermoshaker , Jeff Braman ,Stratagene , ... 1: The tempest Thermoshaker , , ... and a 96-deep-well, round-bottom plate were used ... format. Early- and mid-log phase rates of bacterial growth, ...
... system for inducible mammalian expression , , ... control mammalian expression,system ... a wide range of mammalian cell types. The,inducible promoter ... absence of,the ecdysone analog ponasterone A (ponA). In a ...
... ,Economical quantitative PCR (QPCR) reagent kits ... , Gothami Padmabandu Lisa Grismer ... quantitative PCR and RT-PCR core,reagent kits ... Several versions have been created to optionally ...
Cached Biology Technology:High-Throughput Cultivation of Bacterial Clones 2High-Throughput Cultivation of Bacterial Clones 3Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 2Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 3Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 4Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 5Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 6Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 7Versatile Vectors for Ponasterone A- Inducible Control of Gene Expression in,Mammalian Cells 8Brilliant Core Reagent Kits for QPCR and QRT-PCR 2Brilliant Core Reagent Kits for QPCR and QRT-PCR 3Brilliant Core Reagent Kits for QPCR and QRT-PCR 4Brilliant Core Reagent Kits for QPCR and QRT-PCR 5Brilliant Core Reagent Kits for QPCR and QRT-PCR 6Brilliant Core Reagent Kits for QPCR and QRT-PCR 7Brilliant Core Reagent Kits for QPCR and QRT-PCR 8Brilliant Core Reagent Kits for QPCR and QRT-PCR 9Brilliant Core Reagent Kits for QPCR and QRT-PCR 10Brilliant Core Reagent Kits for QPCR and QRT-PCR 11Brilliant Core Reagent Kits for QPCR and QRT-PCR 12Brilliant Core Reagent Kits for QPCR and QRT-PCR 13Brilliant Core Reagent Kits for QPCR and QRT-PCR 14
(Date:7/11/2014)... addition to the hippocampus, the marginal division of ... memory. What is the impact degree of substance ... and memory function? Yan Yu and his team, ... found, using immunofluorescence staining, that substance P receptor, ... and striatal marginal division of normal rats. Unilateral ...
(Date:7/11/2014)... de Janeiro, Brazil- In the brains of all ... that allows an electric or chemical signal to ... Chemical synapses, which are the most abundant type ... Synapse formation is crucial for learning, memory, perception ... inhibitory synapses critical for brain function. For ...
(Date:7/10/2014)... July 1, 2014 Smart technology emphasis ... convenience and improved security: NXT-ID, Inc. (OTCBB: NXTD), eBay Inc. ... GOOG ), Apple Inc. (NASDAQ: AAPL ), Visa ... MA) NXT-ID, Inc., (OTCQB: NXTD) a biometric authentication company ... that the second series of 30 second spots on CNBC ...
Breaking Biology News(10 mins):Blame it on the astrocytes 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5
... known already for its role in schizophrenia ... according to a report in todays advance on-line issue ... Deaconess Medical Center (BIDMC), the study further suggests that ... diagnostic marker and therapeutic supplement for the treatment of ...
... which glows under ultraviolet light have shown that up ... cleaned. Their findings, published in BioMed Centrals journal, BMC ... bacteria Clostridium difficile (C. difficile) linger in the loo ... Michelle Alfa and a team of scientists from Manitoba, ...
... technology, DALLAS, May 7 An innovator ... will provide its proven public key,infrastructure to the ... on machine readable travel documents (MRTDs),-- also known ... nearly 23 million,citizens by the end of 2008., ...
Cached Biology News:A new gene trigger for pregnancy disorder identified 2UV lotion lights the way to cleaner facilities 2Entrust, Hewlett Packard Partner to Deploy Taiwanese ePassports, Authenticate Biometric Data 2Entrust, Hewlett Packard Partner to Deploy Taiwanese ePassports, Authenticate Biometric Data 3
... Edited by N.C. Price (1996) ... information on protein chemistry and ... of proteins. Data on the ... crystalization, size determination, amino acid ...
... B.S. Dunbar (1994) • This ... principles and methods for preparing samples ... such as radiometric and bioluminescence-enhanced detection ... covered are, the detection and characterization ...
See product name for description....
RAT ANTI MOUSE TCR V BETA 7:RPE Immunogen: T-cell lymphoma TK1...
Biology Products: