Navigation Links
Brilliant Core Reagent Kits for QPCR and QRT-PCR

-template control did not show any amplification (Figure 4).

To evaluate performance, we compared Stratagenes Brilliant single-step QRT-PCR kit to a another commercially available quantitative RT-PCR one-step system by amplifying and detecting a 150-bp region of human GAPDH mRNA using the Molecular Beacon GAPDH Expression Analysis kit. For this comparison, we amplified three concentrations (100, 1, and 0.01 ng) of human total RNA (Methods); however, because the other commercially available kit did not provide a passive reference dye, none was included in this experiment. We found that for each concentration of target RNA, the Ct was comparable or better for the Brilliant kit (data not shown). We assessed the sensitivity of the Brilliant two-step QRT-PCR kit to detect a low-abundance target by amplifying and detecting hypoxanthine phosphoribosyl transferase (HPRT) mRNA from human brain total RNA. For the real-time analysis of the RT-PCR amplification, we used the primers and linear hydrolysis probe from the TaqMan pre-developed assay reagents (PE Biosystems) in the Brilliant Plus two-step QRT-PCR kit. We included UNG and the passive reference dye.The following Ct values were obtained: 22.5 (100 ng), 26.4 (10 ng), 29.1 (1 ng), 32.7 (100 pg), and 37.7 (10 pg) (data not shown). The results show that with the Brilliant Plus two-step quantitative RT-PCR kit, a low-abundance mRNA can be detected from as little as 10 pg of total RNA.

Conclusions

The Brilliant QPCR and QRT-PCR core reagent kits provide highly sensitive and versatile quantitative assessments for real-time gene quantification and gene expression analysis. The kits are compatible with linear hydrolysis (TaqMan probes), molecular beacon, single dye primers, and Scorpions fluorescent technologies. With the Brilliant QPCR core reagent kit, we detected as few as 10 copies of mouse muscle nicotinic ace
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14

Related biology technology :

1. Optimized Imaging of Protein Gels Stained with Coomassie Brilliant Blue Dye
2. A Comparison Study of Lipid Tranfection Reagents in A549, NIH 3T3 and COS-7 Cell Lines
3. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
4. High-Efficiency Transfections Achieved with New Low-Toxicity Reagent
5. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
6. Protocol for RNA Isolation Using TRIzol Reagent with Phase Lock Gel-Heavy
7. TransMessenger Transfection Reagent
8. Effectene Transfection Reagent provides efficient gene delivery to primary neuronal cell cultures
9. Efficient DNA transfection of primary CNS neurons using TransMessenger Transfection Reagent
10. Effectene Transfection Reagent
11. PolyFect Transfection Reagent
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Brilliant Core Reagent Kits for QPCR and QRT PCR

(Date:10/19/2014)... 19, 2014 The Asia-Pacific Bromine ... in Asia-Pacific with analysis and forecast of revenue. ... Pacific Bromine Market report, to get an idea ... a glimpse of the segmentation in the Asia-Pacific ... and figures. , http://www.micromarketmonitor.com/market/asia-pacific-bromine-6741503144.html , ...
(Date:10/19/2014)... October 19, 2014 The report ... & Homeland Security), Technology, Payload, Type & by ... segments the global unmanned ground vehicle market into ... forecasting of market sizes. The report also identifies ... opportunities impacting it along with the adoption trends. ...
(Date:10/18/2014)... The Asian arthroscopic devices report defines and ... forecast of revenue. The arthroscopic devices market in Asia ... at a CAGR of 6.5% from 2013 to 2018. ... devices market, to get an idea of the in-depth ... segmentation of arthroscopic devices market in the same region, ...
(Date:10/18/2014)... (PRWEB) October 18, 2014 According to ... Resource & Well Type - Global Trends & Forecasts ... estimated $31 billion in 2011. This value is expected ... billion by 2017, with 10% CAGR during the same ... hydraulic fracturing production market in terms of hydraulic horse ...
Breaking Biology Technology:The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 2The Asia-Pacific Bromine Market is estimated to grow to $4,080.1 million by 2018 - New Report by MicroMarket Monitor 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 2Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 3Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 4Unmanned Ground Vehicle (UGV) Market Estimated to Reach $8.26 Billion by 2020 - New Research Report by MarketsandMarkets 5The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 2The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 3The Asian arthroscopic devices market is estimated to grow to $712.3 million by 2018 - New Report by MicroMarket Monitor 4Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 2Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 3Hydraulic Fracturing Market Projected To Reach $64 Billion by 2017 - New Report by MarketsandMarkets 4
... 11 Debiopharm Group,(Debiopharm), a global independent ... conditions, today announced that it,received current Good ... chemical, physical and biochemical quality control work,conducted ... the same time,certification of the Lausanne site ...
... the Sustainable Biodiesel Alliance On September 10th, 2007 ... Tour to New York City. Legendary artist Willie Nelson,and ... Square. Dedicated to,its "save the planet" motto, Hard Rock,s ... of the Sustainable Biodiesel Alliance. Award-winning,actors Woody Harrelson and ...
... 10 SGX Pharmaceuticals, Inc.,(Nasdaq: SGXP ) ... collaboration,agreement with Novartis focused on the development and ... first line and drug resistant,Chronic Myelogenous Leukemia (CML). ... progressing a number of,potential drug candidates, with the ...
Cached Biology Technology:Debiopharm Receives cGMP Certification for Quality Control of its Drug Products 2MultiVu Video Feed: Hard Rock's 2007 Ambassadors of Rock Tour Is "On the Road Again" With Music Legend Willie Nelson 2SGX Provides Update on BCR-ABL Collaboration 2SGX Provides Update on BCR-ABL Collaboration 3
(Date:10/15/2014)... , Oct. 15, 2014   Neurotechnology ... today announced the availability of the VeriLook ... Surveillance 3.0 provides real-time biometric face identification using ... digital surveillance cameras. The new version not only ... differentiates people from objects while they are moving ...
(Date:10/14/2014)... is how to produce enough food to feed the ... Agriculture Organization of the United Nations predicts that food ... to feed a growing global population, and plants are ... production. Plants—grains, cereals, fruits, vegetables, and more—feed humans ... must tap into our knowledge of how plants work ...
(Date:10/14/2014)... of researchers, led by the Chinese Academy of Agricultural ... Genetics a brief genomic history of tomato breeding, ... plant. , The C.M. Rick Tomato Genetics Resource Center ... study by providing seed of both cultivated tomato varieties ... on the first tomato genome sequence completed just two ...
Breaking Biology News(10 mins):VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 2VeriLook Surveillance 3.0 SDK Identifies Faces and Moving Objects, Differentiates Pedestrians from Other Moving Objects in Video Surveillance Systems 3Building a bridge from basic botany to applied agriculture 2Building a bridge from basic botany to applied agriculture 3
... DURHAM, N.C. - Immune system cells of the brain, ... in memory and learning, according to new research by Duke ... in psychology & neuroscience, had shown that laboratory rats experiencing ... response to subsequent infections, which also harms their learning and ...
... Altamira caves are stirring controversy over the possibility that ... paintings that changed views about the intellectual ability of ... the current edition of Chemical & Engineering News ... of Stone Age paintings so magnificent that experts have ...
... Bees, bats, and moths all follow their noses in ... such animals for pollination often produce particular chemical scents ... produce certain chemicals is also determined by a plant,s ... ability to respond to pollinator pressures. So which ...
Cached Biology News:Source found for immune system effects on learning, memory 2Source found for immune system effects on learning, memory 3For certain orchids, relatives more important than pollinators in shaping floral attractants 2For certain orchids, relatives more important than pollinators in shaping floral attractants 3For certain orchids, relatives more important than pollinators in shaping floral attractants 4
Anti-Goat HRP-DAB Cell & Tissue Staining Kit...
... The Light Diagnostics Cytomegalovirus (CMV) pp65 Antigenemia ... qualitative identification of lower matrix protein pp65 ... Kit Components: ... One bottle containing 220 mL of buffered ...
... The CultureWell cell culture ... for preparing cultured cells for ... uses medical-grade silicone gaskets preassembled ... cell culture plates. The entire ...
nuclear cap binding protein subunit 1, 80kDa, mRNA...
Biology Products: