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Brilliant Core Reagent Kits for QPCR and QRT-PCR

CR amplification. With the two-step format, which includes both random oligomers and oligo(dT) primers, only a small portion of the reverse transcription reaction is used for PCR amplification. Consequently, cDNA samples can be archived or used for amplifying multiple targets. With the Brilliant Plus two-step QRT-PCR format, UNG can be used for controlling carry-over contamination.

Compatibility with Fluorescent Chemistries

Total RNA

Threshold Cycle (Ct)

100 ng

14.8

10 ng

18.8

1.0 ng

22.1

100 pg

25.6

10 pg

28.5

1.0 pg

32.3

0.1 pg

35.2

We tested the Brilliant single-step QRT-PCR core reagent kits with a number of fluorescent probes to confirm the kits compatibility with different fluorescent chemistries. We used a linear hybridization probe specific for detecting human glyceraldehyde-3-phosphate dehydrogenase (GAPDH) mRNA with the Brilliant single-step QRT-PCR core reagents. The indicated amounts of human total RNA were added. Table 4 shows the real-time analysis of this target titration for the abundant GAPDH mRNA. As expected, both the no-reverse transcriptase (no-RT) control and the no
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