( l cell lines and postmitotic neurons in...)Biolistic Transfection of Organotypic Brain Slices

l cell lines and postmitotic neurons in intact brain tissue. Once the specific parameters of the Biolistic transfection are determined, this method is efficient, reproducible, and does not require advanced molecular biological facilities for its application. Significantly, Biolistic bombardment allows the time and location of transfection to be determined by the age of the animal from which slices are made and allows gene manipulation of individual cells in otherwise normal background tissue, thus circumventing many of the problems of interpreting data from transgenic and knockout animals.

Bibliography
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2. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C., Green fluorescent protein as a marker for gene expression. Science, 263, 802-805 (1994).

3. Jiao, S., Cheng, L., Wolff, J. A. and Yang, N.-S. Particle-bombardment- mediated gene transfer and expression in rat brain tissues. Bio/Technol., 11, 497-502 (1993).

4. Johnston, S. A. Biolistic transformation: microbes to mice. Nature, 346, 776-777 (1990).

5. Klein, T. M., Wolf, E. D., Wu, R. and Sanford, J. C. Highvelocity microprojectiles for delivering nucleic acids into living cells. Nature, 327, 70-73 (1987).

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7. Lo, D. C., McAllister, A. K. and Katz, L. C. Neuronal transfection
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