Navigation Links
Biolistic Transfection of Organotypic Brain Slices

l cell lines and postmitotic neurons in intact brain tissue. Once the specific parameters of the Biolistic transfection are determined, this method is efficient, reproducible, and does not require advanced molecular biological facilities for its application. Significantly, Biolistic bombardment allows the time and location of transfection to be determined by the age of the animal from which slices are made and allows gene manipulation of individual cells in otherwise normal background tissue, thus circumventing many of the problems of interpreting data from transgenic and knockout animals.


Bibliography
1. Arnold, D., Feng, L., Kim, J. and Heintz, N., A strategy for the analysis of gene expression during neural development. Proc. Natl. Acad. Sci. USA, 91, 9970-9974 (1994).

2. Chalfie, M., Tu, Y., Euskirchen, G., Ward, W.W. and Prasher, D.C., Green fluorescent protein as a marker for gene expression. Science, 263, 802-805 (1994).

3. Jiao, S., Cheng, L., Wolff, J. A. and Yang, N.-S. Particle-bombardment- mediated gene transfer and expression in rat brain tissues. Bio/Technol., 11, 497-502 (1993).

4. Johnston, S. A. Biolistic transformation: microbes to mice. Nature, 346, 776-777 (1990).

5. Klein, T. M., Wolf, E. D., Wu, R. and Sanford, J. C. Highvelocity microprojectiles for delivering nucleic acids into living cells. Nature, 327, 70-73 (1987).

6. Klein, T. M., Arentzen, R., Lewis, P. A. and Fitzpatrick- McElligott, S. Transformation of microbes, plants, and animals by particle bombardment. Bio/Technol., 10, 286- 291 (1992).

7. Lo, D. C., McAllister, A. K. and Katz, L. C. Neuronal transfection
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
3. Comparison of Performance Characteristics of Different Biolistic Devices
4. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
5. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
6. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
7. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
10. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
11. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Biolistic Transfection Organotypic Brain Slices

(Date:12/22/2014)... 2014 ITRA Global, one of the ... commercial real estate, has further expanded its global reach ... and Brisbane, Western Australia, reports Mylinda Vick, CCIM, Chairman ... ITRA Global / ACORPP (Australian Corporate Property and Projects) ... providing property services to a wide range of clients ...
(Date:12/19/2014)... MA (PRWEB) December 19, 2014 Charm ... MRLAFMQ test for the Detection of Aflatoxin M1 in ... to receive independent third party validation. The peer reviewed ... Unit of the Institute for Agricultural and Fisheries Research ... Group. , Aflatoxin B1, the most toxic aflatoxin ...
(Date:12/19/2014)... Dec. 19, 2014  Roche (SIX: RO, ROG; OTCQX: ... Inc. (Bina), a privately held company based in ... Bina provides a big data platform for centralized ... data. Bina,s proprietary on-market Genomic Management Solution, Bina-GMS ... to perform fast and scalable analyses to maximize ...
(Date:12/19/2014)... DUBLIN , Dec. 19, 2014 Research ... announced the addition of the "Technology Innovations ... their offering. http://photos.prnewswire.com/prnh/20130307/600769 ... Innovations in Smart Fabrics" offers a detailed assessment ... Key Findings: 1. ...
Breaking Biology Technology:ITRA Global Continues International Expansion in Perth and Brisbane, Australia 2Charm Sciences Achieves Independent Validation of First 15 Minute Quantitative Screening Method for Aflatoxin M1 2Roche acquires Bina Technologies and enters the genomic informatics market 2Roche acquires Bina Technologies and enters the genomic informatics market 3Technology Innovations in Smart Fabrics (Technical Insights) 2
... Cord Blood America, Inc.,(OTC Bulletin Board: CBAI), the ... ) focused on bringing the life,saving potential ... reported that CorCell Companies Inc., Philadelphia, Pa., which ... profitable quarter,ever for the three months ending March ...
... WuXi PharmaTech (NYSE:,WX), a leading pharmaceutical, biotechnology ... with operations in China and the United,States, ... Dr. Peng Wang as Vice,President of Discovery ... Prior to joining WuXi PharmaTech, Dr. ...
... Pharmaceutical Inc.,("Dragon Pharma" or the "Company" TSX: DDD; ... today announced that June 26,2008 has been set ... The Annual Meeting of Dragon stockholders will ... Tower, Fuhua Three Road, Shenzhen, China,at 2:00 p.m. ...
Cached Biology Technology:Cord Blood America Reports CorCell, Its Major Acquisition in 2006, Turns Profitable 2WuXi PharmaTech (NYSE: WX) Appoints Dr. Peng Wang as VP of Discovery Biology 2
(Date:12/11/2014)... , Dec. 09, 2014 Research and ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has ... in India 2015-2019" report to their offering. ... this market is the adoption of multimodal biometric ... characteristic of an individual for verification and identification ...
(Date:12/11/2014)... 10, 2014  Data Sciences International (DSI), the ... a new series of digital telemetry implants to ... M series, part of the PhysioTel™ Digital platform, ... possible physiologic data when incorporating functional endpoints into ... to toxicology studies has evolved from short ECG ...
(Date:12/10/2014)...  Valencell, a leader in performance biometric data sensor ... from its licensees for highly accurate, clinically validated biometric ... coming from fitness and health sectors, but first responders/military ... is only as useful as the biometric data it ... in long-term mass consumer adoption of wearable products," said ...
Breaking Biology News(10 mins):Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2New telemetry implants expected to change how large animal toxicology studies are conducted 2Wearable Technology Products Demand Highly Accurate Biometric Technology 2
... from the European Molecular Biology Laboratory (EMBL) in Heidelberg, ... UK, have revealed new insights into how sex chromosomes ... the fact that males have only one copy of ... enzyme distinguishes between male and female sex chromosomes in ...
... regions of the world, the impact of human activity ... The high world population growth rate and the expansion ... climatic changes (longer periods of drought, irregular rainfall patterns) ... of desertification. According to World Soil Information (ISRIC) rate, ...
... trillions of stowaways in the water held in their ... species could be released into new environments. Disease-causing microbes ... health, according to an article in the May issue ... or skullduggery at work here, said Professor Fred Dobbs ...
Cached Biology News:X chromosome exposed 2Reforestation using exotic plants can disturb the fertility of tropical soils 2Reforestation using exotic plants can disturb the fertility of tropical soils 3Microbial stowaways: Are ships spreading disease? 2
Mouse monoclonal [M2II-12] to MRP2 ( Abpromise for all tested applications). entrezGeneID: 1244 SwissProtID: Q92887...
Mouse monoclonal antibody to ANAPC2 - anaphase promoting complex subunit 2...
... The MAX POWER 500V Programmable ... operate instruments which can handle virtually ... to high current blotting. It is ... text displays. The MAX POWER Programmable ...
The Programmable MP-3500P-F Power Supply can be applied for any Microfluidic application....
Biology Products: