Navigation Links
Biolistic Transfection of Organotypic Brain Slices

d culture dish is placed approximately 9 cm below the launch assembly. A vacuum of 25"-27" Hg is pulled, and the cells are bombarded at a rupture pressure of either 450 or 900 psi. The higher pressure is more effective at propelling the gold particles into the cells; however, it may also cause slightly more cell death. Once the cells have been transfected, the media is replaced gently into the dish. Some cells may detach from the dish at this point, but the majority of them will reattach to the substrate and remain healthy.


Neuronal Cell Line Results
We routinely use biolistics for transfecting into neuronal cell lines (e.g. PC12 cells) various DNA constructs such as those expressing lacZ, Green-Fluorescent Protein (GFP; Chalfie et al., 1994) or the Trk neurotrophin receptors. Additionally, we are able to efficiently co-express combinations of these plasmids, which is useful for confirming successfully transfected cells and normalizing overall transfection efficiency.

Expression of the transfected DNA constructs can be assayed for by their color (such as the marker GFP or the β-galactosidase substrate, X-gal) or by the morphological change they induce in the cells. Following transfection of single or multiple types of DNA constructs, dissociated cells express the appropriate protein products by 24 hours after transfection; expression persists for a minimum of 4 days. In general, the presence of a gold particle in the nucleus of a cell confirms successful transfection. Under optimal conditions, up to 20% peak transfection rates can be achieved.

Atypical result is depicted in Figure 2, where cultured PC12 cells, which normally do not express the neurotrophin recept
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
3. Comparison of Performance Characteristics of Different Biolistic Devices
4. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
5. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
6. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
7. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
10. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
11. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Biolistic Transfection Organotypic Brain Slices

(Date:7/10/2014)... July 10, 2014 OMICS Group’s 5th ... during 18-20 August, 2014 at Double Tree by Hilton ... recent developments in Analytical & Bioanalytical research methods only ... Speaking on this occasion, Dr. Srinubabu Gedela, MD of ... conference is a remarkable one in bringing a unique ...
(Date:7/10/2014)... Ind. , July 10, 2014 /PRNewswire/ ... its use of capnography for respiratory monitoring ... group of healthcare leaders in embracing state-of-the-art ... how effectively patients are breathing and can ... occurs. By measuring the amount of carbon ...
(Date:7/10/2014)... 2014 /PRNewswire-iReach/ -- Tute Genomics, the leader ... an agreement with Lineagen, Inc., to provide ... Dx PLUS.  Lineagen, focused on the ... currently offers FirstStep Dx PLUS, a ... by healthcare providers as a first-line genetic ...
(Date:7/10/2014)... According to the International Atomic Energy Agency (IAEA) ... terrorists acquiring sufficient quantities of plutonium or highly ... explosive device. The IAEA also notes that most ... quantities, which can be challenging to detect with ... study appearing this week in the Journal ...
Breaking Biology Technology:A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 2Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 3Tute Genomics Platform Selected to Provide Clinical Interpretation for Lineagen's NextStepDx PLUS Next-Generation Sequencing-Based Diagnostic Test 4Sensitive detection method may help impede illicit nuclear trafficking 2
... Mass. , July 14 Sermo ( http://sermo.com/client ... Client Center. Based on four years of client feedback and ... meets demands for enhanced navigation, mining and monitoring of organic ... million comments and nearly 4 million votes, Sermo is rich ...
... , AKRON, Ohio , July 13 State and Federal Communications, ... report on how states are responding to the Supreme Court,s landmark decision in ... , , To ... "Citizens United Update" in the lower left of the home page. The report is ...
... of Technology announces a new type of nanopore devices that ... example to read off their sequence. In a paper entitled ... Letters ), they report a novel technique to fabricate tiny ... a thickness of only 1 atom) and they managed to ...
Cached Biology Technology:Sermo Announces New Client Center with Advanced Mining and Monitoring of Organic Physician Dialog 2Sermo Announces New Client Center with Advanced Mining and Monitoring of Organic Physician Dialog 3Report Details How States Respond to Citizens United Decision 2DNA through graphene nanopores 2
(Date:7/10/2014)... CORAL SPRINGS, Florida , July 1, 2014 ... smart wallets pave way for convenience and improved security: NXT-ID, ... Google Inc. (NASDAQ: GOOG ), Apple Inc. (NASDAQ: ... ) and MasterCard Corporation (NYSE: MA) NXT-ID, Inc., (OTCQB: ... market, is pleased to announce that the second series of ...
(Date:7/10/2014)... -- According to the new market research ... Material (Optical Prism, Pizeoelectric, Capacitive, and Adhesives), Application (Mobile ... Finance), and Geography - Global Trends & Forecasts to ... Sensors Market is projected to cross $14.35 Billion by ... 2014 to 2020. Browse more than 78 ...
(Date:7/10/2014)... Sweden , July 8, 2014 ... has won a new design win (DW). An Asian OEM ... date for mass production start in August 2014.   ... Asian smartphone OEM, which has a planned date for start ... an initial ramp order of SEK 5M, for delivery in ...
Breaking Biology News(10 mins):Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 2Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 3Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 4Advantages for Digital Wallets Eliminate Need to Carry Credit Cards - Large Scale CNBC Ad Campaign for Latest Smart Wallet to Commence July 7th 5Fingerprint Sensors Market worth $14.35 Billion by 2020 2Fingerprint Sensors Market worth $14.35 Billion by 2020 3FPC Wins DW and Initial Ramp Order for FPC1021 Touch Sensor for Flagship Smartphone from Asian OEM 2
... do skin cells know when to proliferate and at ... the Journal of Cell Biology , Nguan Soon ... protein called PPARβ/δ to make sure overlying epithelial cells ... between different cell types are critical to maintain the ...
... called quantum tunneling, may aid the quest for accurate, low-cost ... Nanotechnology Letters by Stuart Lindsay and his collaborators at ... a particle, say an electron, can cross a barrier, when, ... to do so. Unraveling the DNA sequences of the ...
... in Technology and Innovation in Global BiometricsGAITHERSBURG, Md., ... LMT ) has been named the Biometrics Systems ... Sullivan, recognizing the Corporation,s leadership in developing innovative ... Frost and Sullivan based the award on a ...
Cached Biology News:Time (and PPAR-beta/delta) heals all wounds 2Time (and PPAR-beta/delta) heals all wounds 3Covering the bases: Quantum effect may hold promise for low-cost DNA sequencing, sensor applications 2Covering the bases: Quantum effect may hold promise for low-cost DNA sequencing, sensor applications 3Covering the bases: Quantum effect may hold promise for low-cost DNA sequencing, sensor applications 4Lockheed Martin Named Biometrics 'Company of the Year' by Frost and Sullivan 2
Applications: Epitope mapping, Mapping protein-protein contacts, Identification of enzyme substrates/inhibitors, Identification of receptor agonists/antagonists...
Bethyl Laboratories packages antibodies, conjugates and calibrators to provide quantitative ELISA kits. Each kit contains the following components, sufficient for 1000 single well assays....
Request Info...
QML offers standard and customized paraffin embedded tissue blocks....
Biology Products: