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Biolistic Transfection of Organotypic Brain Slices

in brain slices using particle-mediated gene transfer. Neuron, 13, 1263-1268 (1994).

8. McAllister. A. K., Lo, D. C. and Katz. L. C., Neurotrophins regulate dendritic growth in developing visual cortex. Neuron, 15, 791-803 (1995).

9. Pecorino, L. T. and Lo, D. C. Having a blast with gene transfer. Curr. Biol., 2, 30-32 (1992).

10. Stoppini, L., Buchs, P. A. and Muller, D., A simple method for organotypic cultures of nervous tissue. J. Neurosci. Meth., 37, 173-182 (1991).

11. Sanford, J. C., Smith, F. D. and Russell, J. A., Optimizing the Biolistic process for different biological applications. Methods in Enzymology 217, 483-509 (1993).


Acknowledgements
We would like to thank Stephen Johnston for his continued support and advice in applying Biolistic transfection to cell lines and brain slices. Thanks also to Regeneron Pharmaceuticals for providing neurotrophins, Trk receptor cDNA, and PC12 cells; to Lloyd Green for his gift of PC12 cell lines; and to Martin Chalfie for providing GFP cDNA.

Biolistic is a registered trademark of E.I DuPont de Nemours and Company. Biolistic technology is exclusively licensed to Bio-Rad Laboratories for commercialization of devices.


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