Navigation Links
Biolistic Transfection of Organotypic Brain Slices

in brain slices using particle-mediated gene transfer. Neuron, 13, 1263-1268 (1994).

8. McAllister. A. K., Lo, D. C. and Katz. L. C., Neurotrophins regulate dendritic growth in developing visual cortex. Neuron, 15, 791-803 (1995).

9. Pecorino, L. T. and Lo, D. C. Having a blast with gene transfer. Curr. Biol., 2, 30-32 (1992).

10. Stoppini, L., Buchs, P. A. and Muller, D., A simple method for organotypic cultures of nervous tissue. J. Neurosci. Meth., 37, 173-182 (1991).

11. Sanford, J. C., Smith, F. D. and Russell, J. A., Optimizing the Biolistic process for different biological applications. Methods in Enzymology 217, 483-509 (1993).


Acknowledgements
We would like to thank Stephen Johnston for his continued support and advice in applying Biolistic transfection to cell lines and brain slices. Thanks also to Regeneron Pharmaceuticals for providing neurotrophins, Trk receptor cDNA, and PC12 cells; to Lloyd Green for his gift of PC12 cell lines; and to Martin Chalfie for providing GFP cDNA.

Biolistic is a registered trademark of E.I DuPont de Nemours and Company. Biolistic technology is exclusively licensed to Bio-Rad Laboratories for commercialization of devices.


back to top
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
3. Comparison of Performance Characteristics of Different Biolistic Devices
4. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
5. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
6. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
7. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
10. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
11. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells

Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Biolistic Transfection Organotypic Brain Slices

(Date:11/6/2009)...OOK, N.Y., Nov. 6 BioSpe...harmaceutical company developing first-in-class co...ncial results for the third quarter ended Septembe...in September of this year, the FDA,s Arthritis Adv... of 12-0, the approval of XIAFLEX(TM) for the trea...
(Date:11/6/2009)...IENNA, Austria, November 6 EUCODIS...nufacturing, and marketing enzymes for the,chemica...o a research,agreement with Bio Sidus, the leading..., The agreement gives EUCODIS Bioscience exclu...t - to Bio Sidus, proprietary collection of,psychr...
(Date:11/6/2009)...RY, Nov. 6 /PRNewswire-FirstCall/ - Oncolytics Bio...the "Company") today announced updated results fro...atients with sarcomas metastatic to the lung in a ...Tissue Oncology Society Meeting held in Miami Beac...resentation, entitled "A Phase II Study of Intrave...
(Date:11/5/2009)...ORTATION -- Gone hybrid . . . , Fuel economy r... now posted at the www.fueleconomy.gov web site,... maintains for the U.S. Department of Energy and t...he top 10 vehicles on the best fuel economy list a...PG city; 48 highway). "This list proves that hybri...
Breaking Biology Technology:Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 2Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 3Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 4Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 5Reissue with Tables: BioSpecifics Technologies Corp. Reports Third Quarter 2009 Financial Results 6EUCODIS Bioscience to Explore Novel Enzymatic Activities in Antarctic Bacteria Library From Bio Sidus 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 2Oncolytics Biotech(R) Inc. Collaborators Present Positive Phase II Sarcoma Trial Results at CTOS Annual Meeting 3November 2009 story tips from the US Department of Energy's Oak Ridge National Laboratory 2November 2009 story tips from the US Department of Energy's Oak Ridge National Laboratory 3
...ent Response to Novel Beta Blocker ...dolol, an investigational,pharmacologically unique...reduces hospitalization and death among heart fail...ing to results of a large DNA study presented,toda...ailure Society of,America. Researchers found that ...
...US$10 million received based on the positive revie...ultiple sclerosis trial - EDMONTON, Sept. 22 /PRN..., a leading developer in the treatment of multiple...ved the US$10 million milestone payment from its,p...ment is based on the previously announced positive...
...l Systems and BrainLAB enabling, non-invasive tr...vous system, PALO...ctors around the globe are turning more and,more t... for the treatment,of cancer and other conditions.... Systems (NYSE: VAR ) and BrainLAB, doctors are p...
Other Biology Technology:Bucindolol Significantly Reduces Hospitalization and Death in Heart Failure Patients With Very Favorable Genotypes 2Bucindolol Significantly Reduces Hospitalization and Death in Heart Failure Patients With Very Favorable Genotypes 3BioMS Medical announces receipt of milestone payment from Eli Lilly and Company 2Non-Invasive Radiosurgery with Novalis Tx(TM) Platform Rolling Out Worldwide 2Non-Invasive Radiosurgery with Novalis Tx(TM) Platform Rolling Out Worldwide 3Non-Invasive Radiosurgery with Novalis Tx(TM) Platform Rolling Out Worldwide 4Non-Invasive Radiosurgery with Novalis Tx(TM) Platform Rolling Out Worldwide 5
(Date:11/4/2009)...HILADELPHIA Although scientists are reluctant to ...n method, evidence continues to grow about its pro...published in Cancer Prevention Research , a journ..., which suggests some reduction in oral cancer. ,...f medicine in the Department of Thoracic/Head and ...
(Date:11/4/2009)...eavy traffic corridors in the cities of Long Beach...roportion of preventable childhood asthma, and the...n the disease has likely been underestimated, acco... California (USC). , The study, which appears i...blic Health , estimated that nine percent of all c...
(Date:11/4/2009)...ancouver, BC - Lowly bacteria, it turns out, hold ...turing plants around the world clean up toxic wast..., specifically RDX, were invented back in World Wa...decades. These high-energy compounds are often us...ful version of TNT. , But with increased knowl...
Breaking Biology News(10 mins):Does green tea prevent cancer? Evidence continues to brew, but questions remain 2USC study finds big air pollution impacts on local communities 2Genomic research will enable greener cleanup of military explosive test sites 2BioCryst Awarded Additional 2477 2 Million by the U S Department of Health 26 Human Services To Develop Peramivir for Influenza 57736 1BioCryst Awarded Additional 2477 2 Million by the U S Department of Health 26 Human Services To Develop Peramivir for Influenza 57736 2BioCryst Awarded Additional 2477 2 Million by the U S Department of Health 26 Human Services To Develop Peramivir for Influenza 57736 3BioCryst Awarded Additional 2477 2 Million by the U S Department of Health 26 Human Services To Develop Peramivir for Influenza 57736 4BioCryst Awarded Additional 2477 2 Million by the U S Department of Health 26 Human Services To Develop Peramivir for Influenza 57736 5DOR BioPharma Announces 249 4 Million NIH Grant Award to Develop Thermostable and Rapidly Acting Vaccines 57734 1DOR BioPharma Announces 249 4 Million NIH Grant Award to Develop Thermostable and Rapidly Acting Vaccines 57734 2DOR BioPharma Announces 249 4 Million NIH Grant Award to Develop Thermostable and Rapidly Acting Vaccines 57734 3DOR BioPharma Announces 249 4 Million NIH Grant Award to Develop Thermostable and Rapidly Acting Vaccines 57734 4DOR BioPharma Announces 249 4 Million NIH Grant Award to Develop Thermostable and Rapidly Acting Vaccines 57734 5DOR BioPharma Announces 249 4 Million NIH Grant Award to Develop Thermostable and Rapidly Acting Vaccines 57734 6AEterna Zentaris Announces Completion of Phase 1 Study with Oral AEZS 112 in Cancer 14165 1AEterna Zentaris Announces Completion of Phase 1 Study with Oral AEZS 112 in Cancer 14165 2AEterna Zentaris Announces Completion of Phase 1 Study with Oral AEZS 112 in Cancer 14165 3
...ug development : only "lucrative","markets" get ex...ve,seen very promising drugs candidates or related...de with it, even if millions would have,been saved...is to find,a drug candidate that affect a "profita...ne. PLoS Medecine has an article about a possible,...
...f the Chinese hybrid rice genome, which was publis...s have recently finished the fine map of the rice ...e of PLoS Biology (Vol.3 Issue 2, 2005), scientist...eported a "much improved, near-complete genome ana...sativa." , The fine maps of the rice genome seque...
...lar signaling mechanism whose existence has been,k...since the,identification of a mutant strain of Dro...Hunt Morgan’s lab in 1910. Later studies,revealed ...t extends,through both sides of the cell membrane ...partner, such as the protein Delta, presented,on t...
Other Biology News:Finding Cures For Tropical Diseases: Is Open Source An Answer? 2Whole genome fine map of rice completed 2Bound for destruction: Ubiquitination protects against improper Notch signaling 2Bound for destruction: Ubiquitination protects against improper Notch signaling 3
Mae II from Roche Applied Science
...etase Activity Kit ADAM17 Activity Kit TNF-α...T FROM LIGHT. AVOID FREEZE/THAW CYCLES. The InnoZy...itive assay designed to measure human TACE activit...creening enzyme inhibitors. An Anti-Human TACE-Coa...
...garose Gel-Digesting Preparation contains a unique...ENTRE for simple, quantitative recovery of intact ...els following electrophoresis in TAE, TBE, MOPS, o...tly in the TAE, TBE, MOPS, and phosphate electroph...
Hot StarTaq Master Mix Kit from QIAGEN
Biology Products: