Navigation Links
Biolistic Transfection of Organotypic Brain Slices

A. Kimberley McAllister, Nina Tang, Lawrence C. Katz, and Donald C. Lo.
Department of Neurobiology, Box 3209, Duke University Medical Center, Durham, NC 27710


Introduction
The introduction of genetic material into cells and tissues of interest remains a rate-limiting step for molecular investigations in many fields, especially neurobiology. Many different methods have been developed to transfect cellsDNA-mediated gene transfer, microinjection, electroporation, lipofection, and viral transfectionand for many cell types, gene transfer using these methods has become a routine tool for studying gene regulation and function. However, many other cell types, particulary postmitotic neurons, have not been easily transfected by these methods.

In the late 1980s, John Sanford, Stephen Johnston, and colleagues developed a device that could potentially allow transfection of such resistant cells (Klein et al., 1987; Johnston, 1990). These inventors called this method biolistics, an anagram for biological ballistics that describes the process of shooting biological materials into living tissue. Biolistic transformation, which involves accelerating DNA-coated microprojectiles directly into cells, was originally designed to circumvent difficulties in transfecting plant cells. In the eight years since the method was first reported, Biolistic bombardment has also been proven effective in transfecting a wide variety of animal tissues as well as in both eukaryotic and prokaryotic microbes, mitochondria, and microbial and plant chloroplasts (Johnston, 1990; Klein et al., 1992; Pecorino and Lo, 1992; Jiao et al., 1993).

To study the signals which regulate neural
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2. Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A
3. Comparison of Performance Characteristics of Different Biolistic Devices
4. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
5. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
6. Highest Transfection Efficiency of an Endotoxin-Sensitive Mammalian Cell Line
7. Optimizing Transfection Conditions for Studying Signal Transduction Pathways
8. Transfection of Green Fluorescent Protein into Human Adrenalcarcinoma Cells
9. Improve Lipid- or Calcium Phosphate-Mediated Transfection of Human Dermal Fibroblasts
10. Versatile Transfection Reagent Offers Low Toxicity and Consistent Performance
11. Low-Toxicity, Lipid-Mediated Transfection of Mammalian Cells
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Biolistic Transfection Organotypic Brain Slices

(Date:7/10/2014)... 2014 Unraveling life’s mysteries can intrigue, ... Archer Family purchased a DNA test to ... up with a lifetime of memories, sharing, and closeness. ... members’ DNA genuinely brought the family closer together. ... into genetic history and prior to taking the ...
(Date:7/10/2014)... 2014 EvoDerma ’s NOOME Anti-Aging Motion ... device now comes with a second treatment cup to help ... The new cup is thinner on the edges for a ... rough, thin and uneven surfaces on the face, neck and ... stimulating treatment on areas such as the cheeks and neck, ...
(Date:7/10/2014)... 2014 On July 9 Prime ... presented Russia,s first national "Industry" ... Company is developing a unique project called MabNext ... a number of innovative drugs based on monoclonal antibodies ... took place at the International Exhibition "Innoprom 2014" in ...
(Date:7/10/2014)... , July 10, 2014 Research ... addition of the "International Photonic Integrated Circuit ... Forecasts to 2019" report to their offering. ... The concept of photonic integration traces its ... century. The promise of photonic integration went unexplored ...
Breaking Biology Technology:Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2
... Md. - Engineers at the University of Idaho have ... upcoming NASA missions that will study the Earth and ... a grant technical officer Pen-Shu Yeh, a senior engineer ... engineering team at the University of Idaho,s ...
... sensitive microscopy developed by researchers at Harvard University could ... scientists to track the location of minuscule metabolites and ... of any kind of fluorescent labeling. , The technique, ... the vibrations in chemical bonds between atoms. SRS microscopy ...
... iPods to those who test their knowledge at www.synbioproject.org ... Among the 75 people Esquire magazine recently chose as ... an emerging discipline that combines science and engineering in order to ... as synthetic biology. , , ...
Cached Biology Technology:New one-of-a-kind technology will fly on 2 NASA missions 2New one-of-a-kind technology will fly on 2 NASA missions 3New label-free method tracks molecules and drugs in live cells 2Synthetic Biology Importance in the 21st Century 2Synthetic Biology Importance in the 21st Century 3
(Date:7/11/2014)... with other group members to reduce aggression and gain ... strategy for grooming activities shows a certain pattern across ... scientific journal Biology Letters . , Grooming between ... without ulterior motives. To be groomed has hygienic benefits ... another individual can provide access to infants, mating opportunities ...
(Date:7/11/2014)... kidney injury related to the use of hydroxyethyl starch ... molecules, according to a report in Anesthesia & ... Society (IARS). , The "total mass of HES molecules" ... renal proximal tubule cells (PTCs), concludes the laboratory study ... Wrzburg, Austria. Other factorssuch as differences in the ...
(Date:7/11/2014)... Evidence exists that cannabinoid receptor type 1 ... Ca2+ influx, and reduce neurotransmitter release. However, ... 1 can increase extracellular Ca2+ influx and ... his team, Tongji Hospital Affiliated to Tongji ... Technology, China used whole cell voltage-clamp and ...
Breaking Biology News(10 mins):Baboons groom early in the day to get benefits later 2In lab studies, hydroxyethyl starch has direct harmful effects on kidney cells 2
... ways to automate the production of DNA and proteins, making ... complex carbohydrates, it,s been a different story. Until now, ... study has been slow and tedious. In what may change ... Georgia has created a method for the rapid chemical synthesis ...
... MADISON The "mineral-breathing" bacteria found in many ... or anaerobic, environments contain microbes sometimes described as "mineral-breathing" ... the same way we use oxygen. According to a ... Nature Geoscience , this bacterial respiration may be accelerated ...
... microbicide based on a new type of anti-HIV drug found ... similar to HIV, according to a study reported at the ... is the first of a gel with an integrase inhibitor, ... for the treatment of HIV but just one of the ...
Cached Biology News:New method for producing 'libraries' of important carbohydrate molecules 2New method for producing 'libraries' of important carbohydrate molecules 3Organic solids in soil may speed up bacterial breathing 2Vaginal gel with integrase inhibitor shows promise in monkeys 2Vaginal gel with integrase inhibitor shows promise in monkeys 3Vaginal gel with integrase inhibitor shows promise in monkeys 4Vaginal gel with integrase inhibitor shows promise in monkeys 5Vaginal gel with integrase inhibitor shows promise in monkeys 6
... Digital Sight DS-L2 imaging controller is a ... DS-L1, and allows users to comfortably observe, ... DS-L2 controller features an 8.4 inch LCD ... variety of Nikon cameras including the DS-Fi1 ...
... a premium quality recombinant form of Thermus ... suitable for a wide range of PCR ... reproducible performance, each preparation is extensively tested ... NovaTaq DNA Polymerase possesses an integral 5′→3′ ...
Mouse polyclonal antibody raised against a partial recombinant TSEN2. NCBI Entrez Gene ID = 80746...
Mouse polyclonal antibody raised against a partial recombinant NCLN. NCBI Entrez Gene ID = 56926...
Biology Products: