Navigation Links
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

and down, and then concentrated by centrifugation. Finally, the pellet was resuspended in 50 l of ethanol (100%). For each bombardment, 10 l of this DNA-gold suspension were loaded onto a macrocarrier.

For experiments with the gene gun, DNA-coating and loading of gold projectiles into Tefzel tubing were done according to Jackstadt et al. (bulletin 2433).

Bombardment and Heat Shock Procedures
For gene delivery experiments with adult schistosomes, approximately 25 males were cultured at 37C in 60 mm petri dishes for 1 day after collecting them from the final host. Immediately before bombardment, the medium was carefully removed, and the worms were placed in the middle of the dish. Biolistics were performed using the following parameters: 1.3502.000 psi helium pressure, target distance stage 1 (3 cm), and 1520"Hg chamber vacuum. After bombardment, medium was added, and the worms were cultivated for an additional 24 hr. Heat shock was applied for 4 hr at 42C.

For biolistic experiments with sporocysts, groups of 2,000 4,000 individuals were pooled and transferred to 35 mm petri dishes. Pressures of 900 and 1,350 psi were applied at a distance of 3 cm and a vacuum of 1520"Hg. After bombardment, 2 ml of culture medium were added, and the larvae were cultured for an additional 48 hr without heat shock.

Molecular and Microscopic Analyses
DNA or RNA for PCR or RT-PCR analyses was isolated from bombarded worms that had been frozen in liquid nitrogen 6 hr after heat shock (Neumann et al. 1993). Protein from bombarded adults was extracted 24 hr after heat shock (Wippersteg et al. 2002). Protein from larval stages was extracted 48 hr after bombardment. SDS-PAGE and western blots were done accor
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2. Comparison of Performance Characteristics of Different Biolistic Devices
3. Biolistic Transfection of Organotypic Brain Slices
4. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
5. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
6. DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
7. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
8. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
9. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
10. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
11. Using XL1-Red Mutator Strain to Generate Esterase Variants
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Biolistic Gene Transfer Generate Transgenic Schistosomes Rev

(Date:7/3/2015)... July 3, 2015 ... september 2015 på  http://www.openinnovationinscience.at till det ... Science" som hålls i Wien ... undersökning med internationella forskare och vetenskapsmän som ... hälsovetenskapens två största utmaningar bristen på incitament ...
(Date:7/2/2015)... ... , ... Cuvettes have been used in countless labs for decades. In the past, people ... As expected, this was a daunting and tiring process looking at endless data tables ... able to navigate the complex catalog structure. , Now thanks to FireflySci, scientists are throwing ...
(Date:7/2/2015)... PALO ALTO, Calif. (PRWEB) , ... July 02, 2015 , ... ... designed to improve an individual’s quality of sleep, launched its Indiegogo campaign on June ... and functionality: Luzi's voice command technology makes it effortless to use, while the integration ...
(Date:7/1/2015)... BURLINGTON, Mass. , July 1, 2015 /PRNewswire/ ... three most frequently cited advantages of Otezla are ... favorable safety profile, as reported by surveyed rheumatologists. ... and launched for the treatment of active psoriatic ... . The current standard of care includes conventional ...
Breaking Biology Technology:Lansering av världens första program för öppen innovation för att utbilda forskare 2Lansering av världens första program för öppen innovation för att utbilda forskare 3New Cuvette E-Commerce Website Launched by FireflySci 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 2Luzi Smart Lamp Recently Launched Indiegogo Campaign 3Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 2Surveyed Rheumatologists Report That Otezla's Oral Administration is its Single Greatest Advantage Over Currently Available Treatment Options 3
... Kan., Feb. 28 Aided by the Kansas,Bioscience ... go national,with specialized training programs that will keep ... continue enjoying the benefits of,cutting-edge, world-class research to ... have grown significantly in recent,years, leading to unprecedented ...
... BCRX ) today announced that its fourth quarter ... Tuesday, March 4,2008. At 8:30 a.m. Eastern Time BioCryst ... financial results and provide an update on the,Company,s programs ... by Jon,P. Stonehouse, BioCryst,s Chief Executive Officer, and Stuart ...
... of biotech contract service providers, INDIANAPOLIS, ... the life sciences, has released an extensive ... biotechnology industries,holding significant promise for Indiana,s continued ... launched a new Web site --, http://www.biocrossroadslinx.com ...
Cached Biology Technology:K-State Preparing to Share Expertise With Bioscientists 2BioCryst Pharmaceuticals to Announce Fourth Quarter and Fiscal Year 2007 Financial Results on March 4, 2008 2BioCryst Pharmaceuticals to Announce Fourth Quarter and Fiscal Year 2007 Financial Results on March 4, 2008 3BioCrossroads Launches www.biocrossroadslinx.com and Releases Report on Biopharma Discovery and Development Contract Services 2BioCrossroads Launches www.biocrossroadslinx.com and Releases Report on Biopharma Discovery and Development Contract Services 3BioCrossroads Launches www.biocrossroadslinx.com and Releases Report on Biopharma Discovery and Development Contract Services 4
(Date:6/25/2015)... -- According to a new market ... Technology, Material (Optical Prism, Piezoelectric, Capacitive & Adhesives), ... & Geography - Global Forecast to 2014 - ... expected to reach $14,500.07 Million by 2020, at ... 76 market Tables and 109 Figures spread through ...
(Date:6/25/2015)...  TAKE Solutions Ltd., a global business technology solutions ... Patent and Trademark Office (USPTO) for its "Method for ... Clinical Accelerators to reduce the time taken to standardize ... without the accelerators), thus reducing time to market. ... the heart of the patented Clinical Data Standardization Process ...
(Date:6/24/2015)... , June 24, 2015 This report provides ... the next six years. It contains an analysis of ... along with their impact from the short, medium, and ... discusses the industry, market, and technology trends that currently, ... need of concerned authorities to efficiently manage the increasing ...
Breaking Biology News(10 mins):Fingerprint Sensors Market Worth $14,500.07 Million by 2020 2Fingerprint Sensors Market Worth $14,500.07 Million by 2020 3Fingerprint Sensors Market Worth $14,500.07 Million by 2020 4TAKE Solutions Awarded Patent By USPTO 2Automatic Identification System Market by Class, by Platform, by Application and by Geography - Global Forecasts & Analysis to 2014 - 2020 2
... is the target of potent antibiotics such as penicillin ... of carbohydrate chains, linked together by peptides, which wrap ... according to research conducted by scientists at the California ... cell-wall structure in three dimensions was made possible by ...
... mentally, but genetically everyone is very similar. That,s ... But with population research becoming more and more ... concerned that genetic research could awaken racist attitudes. ... geneticist James Watson claimed there are genes responsible ...
... The International Commission for the Conservation of Atlantic ... Marrakech, Morocco to make decisions on measures to save ... this year with mounting evidence of the impending collapse, ... Mediterranean bluefin tuna fishery is an "international disgrace" and ...
Cached Biology News:Caltech researchers get first 3-D glimpse of bacterial cell-wall architecture 2Could genetic research awaken racist attitudes? 2Tuna Commission to decide the future of Mediterranean bluefin 2
Antibodies were affinity purified using epitopes specific to XLF immobilized on solid support....
... Clone/PAD: ZMD.460. Immunogen: Synthetic peptide ... human C35 protein (chromosome 17 open reading ... Specific for the C35 protein. On ... at ~15 kDa band. Reactivity: Human ...
Immunogen: Heat inactivated bacterial cells of Salmonella enteriditis,paratyphi and typhimurium Storage: 4 C...
... Sepharose 4B, 10 ... purification of calmodulin-binding ... of calmodulin-regulated proteins ... cells. Category: Chromatography ...
Biology Products: