Navigation Links
Biolistic Gene Transfer to Generate Transgenic Schistosomes, Rev A

Volker Wippersteg, Katja Kapp, Werner Kunz, and Christoph G Grevelding, Institute for Genetics, Center of Biological and Medical Research, Heinrich-Heine-University, D-40225 Dsseldorf, Germany


Introduction
Schistosomes are parasitic helminths that cause bilharzia (schistosomiasis) in humans, a tropical disease of worldwide significance (Savioli et al.1997). One focus of international research on such pathogens is to increase the understanding of their molecular biology and to identify and characterize genes with key cellular functions. These genes could be new targets for the development of drugs and vaccines (Johnston et al. 1999).

Gene transfer in homologous systems has been shown to be a powerful tool for the functional analysis of genes and their regulatory elements (Buono and Linser 1992). However, there is comparatively little documentation of attempts to transform multicellular parasites. The goal of our study was to establish a gene delivery system for Schistosoma mansoni, a digenean trematode with a complex life cycle. Specifically, we used biolistic gene transfer techniques to transiently transform adult and larval stages of the schistosome.

Biolistic gene transfer, also known as particle bombardment, was initially designed to transform plants; however, several other types of organisms have been successfully transformed using the Helios gene gun and the PDS-1000/He particle delivery system, both from Bio-Rad (Williams et al. 1991, Schiedlmeier et al. 1994, Davis et al. 1999, Jackstadt et al. 1999, Hara et al. 2002). The principle of biolistics is based on the acceleration of DNA- or RNA-coated microparticles by high-pressure helium. These particles are able to
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9

Related biology technology :

1. Using the VersaFluor Fluorometer to Quantitate GUS Expression in Plant Tissues Bombarded With the Biolistic PDS-1000/He System
2. Comparison of Performance Characteristics of Different Biolistic Devices
3. Biolistic Transfection of Organotypic Brain Slices
4. Optimization of Biolistic Transformation Using the Helium-Driven PDS-1000/He System
5. Sub-Micron Gold Particles Are Superior to Larger Particles for Efficient Biolistic Transformation of Organelles and Some Cells
6. DNA/RNA Blotting With the Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell
7. UV Nicking of DNA in Agarose Gels for Enhanced Transfer and Detection of Megabase Size DNAs
8. Increased Transfer Efficiency Using a Discontinuous Buffer System With the Trans-Blot SD Cell
9. New Kit Generates Exceptionally Pure Total RNA for RT-PCR
10. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
11. Using XL1-Red Mutator Strain to Generate Esterase Variants
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Biolistic Gene Transfer Generate Transgenic Schistosomes Rev

(Date:1/22/2015)... NH (PRWEB) January 22, 2015 GEA ... a table top laboratory homogenizer, the PandaPLUS 2000, which ... nanodispersions, nanoemulsions , and cell disruption . This ... dairy products, fruit juices, liquid food, food additives and ...
(Date:1/22/2015)... OH (PRWEB) January 22, 2015 Crystal Diagnostics ... today that it has received AOAC-PTM Certifications for the six ... O121, and O145; collectively referred to as STEC or the ... 1 colony forming unit (cfu) per 325 g of raw ...
(Date:1/22/2015)... Geneva, Switzerland (PRWEB) January 22, 2015 ... for characterized mammalian Research Cell Banks (RCBs) used for ... generated Research Cell Banks will include Next-Generation Sequencing ... cell’s genetic architecture de-risks biologic manufacturing by ensuring the ...
(Date:1/22/2015)... , Jan. 22, 2015   GenoSpace , a precision ... tools to enable the broad use of genomic, imaging and ... the appointment of Michelle Munson , CEO of Aspera, ... Logo - http://photos.prnewswire.com/prnh/20150122/170713 ...
Breaking Biology Technology:GEA Niro Soavi Announces the PandaPLUS 2000 Homogenizer for Processing New Applications for Nanoemulsions and Cell Disruption 2Crystal Diagnostics Awarded AOAC-PTM Accreditation for the Rapid Detection of “Big 6” E.coli Food Pathogens 2Selexis Generated Research Cell Banks Now Fully Sequenced Using Next-Generation Sequencing 2GenoSpace Expands Board with Appointment of Michelle Munson 2
... Zimmer Holdings, Inc. (NYSE: ZMH ; SWX: ... (OMeGA) has chosen its 2009 grant recipients. OMeGA ... medical education to which Zimmer committed $2 million in ... , OMeGA received more than 250 applications for its ...
... Corporation (NYSE: SYK ) learned today that a federal ... indictment charging Stryker Biotech LLC and certain current and former ... the U.S. Food and Drug Administration (FDA), distribution of a ... disclosed in March of this year that its Biotech division ...
... , INCLINE VILLAGE, Nev., Oct. 28 PDL BioPharma, ... it has priced a $300 million securitization transaction intended ... royalties. , Upon closing of the transaction, which is ... will sell to QHP Royalty Sub LLC ("QHP"), a ...
Cached Biology Technology:Zimmer Holdings Announces OMeGA Graduate Medical Education Grant Recipients 2Zimmer Holdings Announces OMeGA Graduate Medical Education Grant Recipients 3Zimmer Holdings Announces OMeGA Graduate Medical Education Grant Recipients 4Stryker Statement in Response to the Indictment of Its Biotech Division 2PDL BioPharma Announces Pricing of $300,000,000 Securitization Transaction 2PDL BioPharma Announces Pricing of $300,000,000 Securitization Transaction 3
(Date:12/22/2014)... 2014 Research and Markets ( http://www.researchandmarkets.com/research/fx4bg3/the_global ... "The Global Watermarking and Fingerprinting Markets" report ... http://photos.prnewswire.com/prnh/20130307/600769 This insight provides an ... fingerprinting markets. Watermarking aims to control and monitor ...
(Date:12/19/2014)... Inc., the leading personal genetics company, today announced the publication ... of individuals from across the United States ... hundred years ago, the United States ... continents. This study illuminates how American history and the ongoing ...
(Date:12/17/2014)... 15, 2014  HITLAB SM announced today its completion ... its adherence to current U.S. Food and Drug Administration ... smart device and smart phone application trials utilizing the ... "HITLAB is determined to improve global healthcare access, ...
Breaking Biology News(10 mins):The Global Watermarking and Fingerprinting Markets 223andMe Study Sketches Genetic Portrait of the United States 223andMe Study Sketches Genetic Portrait of the United States 323andMe Study Sketches Genetic Portrait of the United States 4HITLAB Completes GCP Audit for FDA Smart Device & App Compliance 2
... and other institutions declare that improvements in management ... the number of tigers currently existing in the region. ... the most recent edition of the journal Biological Conservation. ... Indian subcontinentcomprising India, Bangladesh, Bhutan, and Nepal. It found ...
... Topics include: continental deformation and the San Andreas ... rocks between Britain and America; environmental stress and ... in Canada,s Mackenzie River basin as a CO2 ... in the absence of active volcanism; and geologic ...
... a lot about you. Now, scientists say that the ... likely reveals a lot about you and your brain, too. ... Center, worms that are hermaphrodites (with characteristics of both females ... the other of the two sexes in these worms ...
Cached Biology News:WCS study finds potential to double tiger numbers in South Asia 2November GEOLOGY and GSA TODAY Media Highlights 2November GEOLOGY and GSA TODAY Media Highlights 3November GEOLOGY and GSA TODAY Media Highlights 4November GEOLOGY and GSA TODAY Media Highlights 5November GEOLOGY and GSA TODAY Media Highlights 6November GEOLOGY and GSA TODAY Media Highlights 7November GEOLOGY and GSA TODAY Media Highlights 8November GEOLOGY and GSA TODAY Media Highlights 9November GEOLOGY and GSA TODAY Media Highlights 10Worms take the sniff test to reveal sex differences in brain 2Worms take the sniff test to reveal sex differences in brain 3
BAFF-R (CT) BR3 Affinity Purified Polyclonal Antibody...
Goat polyclonal to NIR1 ( Abpromise for all tested applications). Antigen: Synthetic peptide: SWARGPPKFESVP, corresponding to C terminal amino acids 962-974 of NIR1. Entrez Gene ID: 83394 ...
PMM2 Immunogen: PMM2 (NP_000294, 47 a.a. ~ 112 a.a) partial recombinant protein with GST tag. Storage: -20 C, Avoid Freeze/Thaw Cycles *Available for distribution in the US only...
HOXC11 Antibody...
Biology Products: