Navigation Links
Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine ,,, in Bovine Plasma using the TSQ Quantum Mass Spectrometer

pg/L
Figure 4d: Plasma spiked at 0.5 pg/L LC/MS/MS Chromatograms of Bovine Plasma Spiked with Paroxetine and Internal Standard Quality Control samples at 0.3 and 25 pg/L Figure 5a: Quality Control at 0.3 pg/L

Figure 5b: Quality Control at 25 pg/L Results The chromatography (Figure 4a4d and 5a5b) was capable of resolving Paroxetine and De-Fluoro-Paroxetine from any matrix peaks and was suitable for the quantitation of unknowns.

A calibration curve was constructed from the first and eighth replicate set of calibration standards (Figure 6). The linear fit calibration curve, weighted 1/X, of area ratio plotted against concentration was used to calculate the concentrations of all calibration standards and quality control samples throughout the analytical batch.

A correlation coefficient of r2=0.9994 with an equation of Y=0.00346891 +0.0312025*x was obtained for the curve.

The internal standard peak area response was 3.1% cv for 121 injections (Figure 7). Figure 6: Paroxetine/Int Std area ratio

Figure 7: Internal Standard area response The calibration curve was used to calculate the values of the eight replicates at each level of quality control sample (Table 1) and of the remaining six replicates of standards (Table 2). Table 1: Intra-batch accuracy and precision
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. A New PCR-based Mycoplasma Detection Method
2. DNA-free A NEW Method to Remove DNA
3. RNase Activity in Mouse Tissue: Classification, Hierarchy, and Methods for Control
4. Comparison of Different Methods of mRNA Quantitation
5. Rapid Method Development With the BioLogic DuoFlow Chromatography System for the Purification of His-Tagged Proteins
6. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
7. Comparison of PCR Kleen Spin Columns to Traditional Methods for Purification of PCR Products Prior to Sequencing, Rev A
8. Commercial Plasmid Preparation Methods Yield DNA With Different Transfection Capabilities
9. Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A
10. A Rapid Method for the Purification of Analytical Grade Proteins From Plants Using Preparative SDS-PAGE and Preparative IEF
11. A Robust LC/MS/MS Method for the Quantitation of Very Long Chain Fatty Acids for Research into the Characterization of Peroxisomal Disorders
Post Your Comments:
(Date:8/27/2015)... -- The National Necrotizing Fasciitis Foundation (NNFF) ... doctors at Vanderbilt University Hospital in Nashville, ... Dr. John Crew , director of the ... in Daly City, CA. ... by Dr. Crew, that had previously saved the lives ...
(Date:8/27/2015)... , Aug. 27, 2015 HUYA Bioscience International ... of the Japan Society of HTLV-1, held this week ... The purpose of the Society is to promote research ... development of medical technology and research related to these ... "HUYA is proud to support this prestigious ...
(Date:8/27/2015)... ... August 27, 2015 , ... ... and expertly narrows down the product choices to a manageable shortlist of bench ... the list to models that fit best with the customer’s industry and application. ...
(Date:8/26/2015)... Manhattan, KS (PRWEB) , ... August 26, 2015 ... ... & Biologic Solutions, announces today that a second US laboratory is to open ... in accordance with the continued partnership and long-term growth of research and development ...
Breaking Biology Technology:Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 2Patient's Life and Eyes Saved from Gruesome Flesh-Eating Infection Consuming Her Face 3HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 2HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 3HUYA Bioscience International Sponsors Japan Society Of HTLV-1 Annual Meeting 4METTLER TOLEDO Updates Online Scale Selection Tool 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 2MediVet Biologics Animal Health Secures Additional Laboratory Facility in Manhattan, Kansas 3
... Inc.,(Nasdaq: ENMD ), a clinical-stage pharmaceutical company ... diseases, today,announced that it has received approval from ... of its common stock from,the Nasdaq Global Market ... effective as of the opening of the market ...
... II DATA FOR LEAD DRUG CANDIDATE ARIKACE(TM) DRIVES,CONTINUED ... Transave, Inc., a,biopharmaceutical company focused on developing ... lung diseases,today announced that it has secured a ... Horizon Funding Company LLC, an affiliate of Horizon,Technology ...
... children,s hospitals in nation selected as Top Hospital ... by the Leapfrog Group, ... of only seven children,s hospitals in the nation to have been,selected as ... more than 1,220 participating hospitals., Children,s Hospital,s recognition was based on ...
Cached Biology Technology:EntreMed Announces Stock Listing Transfer to Nasdaq Capital Market 2EntreMed Announces Stock Listing Transfer to Nasdaq Capital Market 3Transave, Inc. Secures $12.5 Million in New Financing 2Transave, Inc. Secures $12.5 Million in New Financing 3Transave, Inc. Secures $12.5 Million in New Financing 4Transave, Inc. Secures $12.5 Million in New Financing 5Children's Hospital of Pittsburgh of UPMC Receives Prestigious Recognition for Patient Safety Efforts in National Survey 2Children's Hospital of Pittsburgh of UPMC Receives Prestigious Recognition for Patient Safety Efforts in National Survey 3
(Date:8/20/2015)... 2015 NXT-ID, Inc. (NASDAQ: NXTD ) ... on the growing mobile commerce market and creator of ... Smart Card Alliance and the EMV Migration Forum.  ... Smart Card Alliance Latin America (SCALA) Chapter provides NXT-ID, ... thought leaders promoting adoption of smart card technology. ...
(Date:8/12/2015)... Aug. 12, 2015   MedNet Solutions , an ... spectrum of clinical research, is proud to announce that ... comprehensive SaaS-based eClinical technology platform, has led the way ... two quarters of 2015.   Q2 2015 and Q1 ... measured by contract value sold in the company,s 15 ...
(Date:8/10/2015)... Germany , August 10, 2015 /PRNewswire/ ... world leader in Eye Tracking Technology for more than ... Eye Tracking Platform for integration into all consumer display ... designs for seamless integration of eye tracking into consumer ... and augmented reality smart glasses. Omnivision,s leading sensor technology ...
Breaking Biology News(10 mins):NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4MedNet Solutions Experiences Explosive Corporate Growth 2SMI Completes OEM Eye Tracking Platform to Cover All Consumer Displays 2SMI Completes OEM Eye Tracking Platform to Cover All Consumer Displays 3
... Fla. --- When it comes to public access, the tree ... University of Florida researchers shows about 70 percent of published ... unable to get to critical data they may need to ... disease control. Scientists are using the genetic data ...
... FORENSICS Mass grave detection . . . ... may gain closure, and killers may receive appropriate punishment, ... clandestine graves. While investigators can find some graves, perhaps ... of Tennessee,s Forensic Anthropology Center and Oak Ridge National ...
... researchers have shown that they can turn genes on or ... is copied into messenger RNA an advance that could ... The technique could also make it easier to engineer ... detect disease, says Timothy Lu, an assistant professor of electrical ...
Cached Biology News:UF scientists encounter holes in tree of life, push for better data storage 2UF scientists encounter holes in tree of life, push for better data storage 3September 2013 story tips 2September 2013 story tips 3An easier way to control genes 2An easier way to control genes 3
Rat Antibody to Mouse TCR VB8.1,8.2:FITC Recognises the V beta 8.1 and V beta 8.2 determinants of the murine T cell receptor. Specificity: mouse TCR VB8.1 and 8.2...
... anti-Human Alpha 1 Acid Glycoprotein ... Goats were immunized with ... in Freund's adjuvant. The ... to ensure specificity. For ...
...
... This DuoSet ELISA Development kit contains ... of sandwich ELISAs to measure natural and ... in cell culture supernates and serum.1 Each ... on approximately fifteen 96-well plates, provided that ...
Biology Products: