of Acetonitrile, centrifuged at 13,000 rpm for 10 minutes and the supernatants decanted. The extracts were evaporated under a stream of nitrogen gas and reconstituted in 100 L of Water/Methanol/Acetic acid (80/20/0.1 v/v/v).

Sample Analysis: The plasma extracts were chromatographed (Thermo Finnigan Surveyor System) on a C18 Elite 100 mm 2.1 mm column (Thermo Hypersil-Keystone) at a flow rate of 250 L/min with a linear gradient of 10% Solvent B (methanol containing 0.1% acetic acid) to 95% B over five minutes. Solvent A was water containing 0.1% acetic acid. Injection volumes of 10 L were used. MS Conditions: Mass spectrometer: Thermo Finnigan TSQ Quantum
Ionization mode: Electrospray (ESI), positive ion
Resolution: 0.7 Dalton Full Width Half Maximum on Q1 and Q3
Selected Reaction Monitoring:
Paroxetine 330.20 > 192.1 +/- 0.3 Da, 22 V collision energy
De-Fluoro-Paroxetine 312.20 > 174.1 +/- 0.3 Da, 20 V collision energy
Argon collision gas pressure 1.5 mTorr

Figure 2: Paroxetine

Figure 3: De-Fluoro Paroxetine

LC/MS/MS Chromatograms of Bovine Plasma Spiked with Paroxetine and Internal Standard Blank Plasma,Plasma containing Internal Standard,Calibration Standards at 0.1 and 0.5 pg/L Figure 4a: Blank Plasma
Figure 4b: Plasma containing Int Std

Figure 4c: Plasma spiked at 0.1
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