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Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine ,,, in Bovine Plasma using the TSQ Quantum Mass Spectrometer

of Acetonitrile, centrifuged at 13,000 rpm for 10 minutes and the supernatants decanted. The extracts were evaporated under a stream of nitrogen gas and reconstituted in 100 L of Water/Methanol/Acetic acid (80/20/0.1 v/v/v).

Sample Analysis: The plasma extracts were chromatographed (Thermo Finnigan Surveyor System) on a C18 Elite 100 mm 2.1 mm column (Thermo Hypersil-Keystone) at a flow rate of 250 L/min with a linear gradient of 10% Solvent B (methanol containing 0.1% acetic acid) to 95% B over five minutes. Solvent A was water containing 0.1% acetic acid. Injection volumes of 10 L were used. MS Conditions: Mass spectrometer: Thermo Finnigan TSQ Quantum
Ionization mode: Electrospray (ESI), positive ion
Resolution: 0.7 Dalton Full Width Half Maximum on Q1 and Q3
Selected Reaction Monitoring:
Paroxetine 330.20 > 192.1 +/- 0.3 Da, 22 V collision energy
De-Fluoro-Paroxetine 312.20 > 174.1 +/- 0.3 Da, 20 V collision energy
Argon collision gas pressure 1.5 mTorr

Figure 2: Paroxetine

Figure 3: De-Fluoro Paroxetine

LC/MS/MS Chromatograms of Bovine Plasma Spiked with Paroxetine and Internal Standard Blank Plasma,Plasma containing Internal Standard,Calibration Standards at 0.1 and 0.5 pg/L Figure 4a: Blank Plasma
Figure 4b: Plasma containing Int Std


Figure 4c: Plasma spiked at 0.1
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