( elines issued by the US Food a...)Bioanalytical Method Intraday Validation for the Quantitation of Paroxetine ,,, in Bovine Plasma using the TSQ Quantum Mass Spectrometer

elines issued by the US Food and Drug Administration.

The aim of this investigation was to demonstrate the sensitivity, linearity, and robustness of the TSQ Quantum to allow researchers to achieve excellent accuracy and precision for highthroughput quantitation of analytes in biological matrices. Paroxetine spiked into bovine plasma was injected as an intra-day batch consisting of blanks, calibration standards, and QCs at eight replicates. TSQ Quantum Tune View Optimization of Paroxetine Paroxetine (C₁₉H₂₀FNO₃, molecular weight 329.37) was infused, 0.1 ng/L, into the ESI source and the four most abundant product ions for the MS/MS breakdown were determined using the automated compound optimization procedure in the TSQ Quantum (Figure 1).

The transition yielding the most abundant product ion (m/z 192.1) was used for the analysis of Paroxetine. Figure 1: Automated optimization of MS/MS parameters for Paroxetine Experimental Conditions Sample Preparation: Aliquots (1 milliliter) of Bovine Plasma (Sigma P4639) were spiked with Paroxetine (Figure 2) in the concentration range of 0.1, 0.2, 0.5, 1, 2, 5, 10, 50, 100, 200, 500 and 1000 pg/L for the calibration standards and at levels of 0.3, 25 and 750 pg/L for quality control samples. De-Fluoro-Paroxetine (Internal Standard, Figure 3) was spiked at a concentration of 25 pg/L. Blank Bovine plasma and plasma containing internal standard were also prepared. Eight replicate plasma aliquots of 100 L were taken from each spiked level and protein precipitated using 250 L
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