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m using an RNA transfection reagent. TransMessenger Transfection Reagent from QIAGEN efficiently delivers siRNA to cells for successful gene knock down (Figures 1 and 2).

Once inside the cell, current models suggest that siRNA interacts with endogenous cellular proteins to form a proteinRNA complex known as the RNA induced silencing complex (RISC). Due to the sequence homology between the siRNA and the target mRNA, the RISC specifically targets the mRNA for enzymatic degradation (see flowchart).
Efficient Gene Silencing Using QIAGEN siRNA and TransMessenger Reagent Efficient, Targeted Gene Silencing Figure 1 Western blot of extracts from transfected HeLa-S3 cells probed with lamin A/C monoclonal primary antibodies. Cells were transfected using the indicated reagents. siRNA: short interfering RNA duplex targeting lamin A/C at position 608630 relative to the start codon; TM: TransMessenger Transfection Reagent; ODN: Scrambled 24mer oligodeoxynucleotide (control). Figure 2 Relative expression levels of lamin A/C after siRNA transfection. The value for the mock transfection was set as 1. Data were normalized to the expression level of GAPDH (internal control), which was quantified by real-time RT-PCR using OmniscriptReverse Transcriptase and the QuantiTect SYBR Green PCR Kit. Expression levels were calculated using a standard curve generated using different amounts of HeLa S3 cDNA. Data obtained under the in
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Related biology technology :

1. BioRobot MDx Workstation
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