The dynamic range of the ELISA used in this study was significantly narrower than that of the Bio-Plex assays. The ELISAs detected 1.6 to 600 pg/ml cytokines, while Bio-Plex assays detected 1.6 to 32,000 pg/ml.
In this study, we were able to demonstrate that testing PBMC samples from asthmatic and healthy children for IL-5, IL-10, IL-13, and IFN-γ by ELISA and Bio-Plex assays yielded similar results. Bio-Plex assays have broader dynamic range and allow for multiplex analysis of cytokines in a single sample. Therefore, Bio-Plex assays have an advantage over the current ELISA methodology when multiple samples have to be analyzed for several cytokines and when available sample volume is limited.
We thank Ulrika Bengtsson, Anne-Marie Fornander, Lena Lindell, Ing-Marie Sandberg, and Kristina Warstedt, Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, for excellent technical assistance.
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