Navigation Links
Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

s obtained in the Bio-Plex assays (see figure). Correlation coefficients (r2) ranged from 0.80 to 0.99 (p<0.001 for all analyses).

The dynamic range of the ELISA used in this study was significantly narrower than that of the Bio-Plex assays. The ELISAs detected 1.6 to 600 pg/ml cytokines, while Bio-Plex assays detected 1.6 to 32,000 pg/ml.


Conclusions
In this study, we were able to demonstrate that testing PBMC samples from asthmatic and healthy children for IL-5, IL-10, IL-13, and IFN-γ by ELISA and Bio-Plex assays yielded similar results. Bio-Plex assays have broader dynamic range and allow for multiplex analysis of cytokines in a single sample. Therefore, Bio-Plex assays have an advantage over the current ELISA methodology when multiple samples have to be analyzed for several cytokines and when available sample volume is limited.


Acknowledgements
We thank Ulrika Bengtsson, Anne-Marie Fornander, Lena Lindell, Ing-Marie Sandberg, and Kristina Warstedt, Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, for excellent technical assistance.

References
Bttcher MF et al., Allergen-induced cytokine secretion in atopic and non-atopic asthmatic children, Pediatr Allergy Immunol, in press

Jenmalm MC et al., Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children, Clin Exp Allergy 31, 15281535 (2001)

OGarra A and Murphy K, Role of cytokines in determining T-lymphocyte function, Curr Opin Immunol 6, 458466 (1994)

Vignali DA, Multiplexed particle-based flow cy
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
2. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
3. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
4. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
5. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
6. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
8. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
9. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
10. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
11. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
Post Your Comments:
(Date:10/7/2008)...ected to aid drug discovery by allowing for the ra...gle reaction, ST. LOUIS, Oct. 7 /PRNewswire-First...o announce that it has signed an exclusive licensi...bana-Champaign allowing,Sigma-Aldrich to offer res...ogates for use in Suzuki-Miyaura coupling to resea...
(Date:10/7/2008)...s to focus on new patient data supporting the Ross...ynerGraft processed human heart .../- CryoLife, Inc. (NYSE: C...ocessing company, announced today,that cardiovascu...e in the,Ross Summit 2008, a two-day surgical cong...
(Date:10/7/2008)...Institutes of Health, The Infectious Disease Resea...Launch and Board of Advisors, SEATTLE, Oct. 7 /PR...,Initiative today announced its first acquisition ...osis (TB) drug candidates. Agreements were reached...nd the Microbial Chemistry,Research Foundation (MC...
(Date:10/7/2008)... Oct. 7 /- BioEnergy International, L...r and manufacturer of,next-generation, renewable b...f Norman R. Augustine to its Board of Advisors eff...f distinguished leadership and innovation,at the h...g in his role as,Chairman and Chief Executive Offi...
Breaking Biology Technology:Sigma-Aldrich and the University of Illinois Offer New Boronic Acid Surrogates to Researchers Worldwide Through Licensing Agreement 2Sigma-Aldrich and the University of Illinois Offer New Boronic Acid Surrogates to Researchers Worldwide Through Licensing Agreement 3CryoLife Hosts Worldwide Surgical Congress for the Ross Procedure 2CryoLife Hosts Worldwide Surgical Congress for the Ross Procedure 3CryoLife Hosts Worldwide Surgical Congress for the Ross Procedure 4Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 2Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 3Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 4Update: The Lilly TB Drug Discovery Initiative Begins Work on New Compounds to Fill Early-Stage TB Drug Pipeline 5BioEnergy International, LLC Announces Norman R. Augustine to Join Advisory Board 2BioEnergy International, LLC Announces Norman R. Augustine to Join Advisory Board 3
... - Last week Gov. Jim Doyle announced a new eHealt...l work to further the adoption of electronic medic... has yet to harness the power of information techn...re system - patient treatment," Doyle said in a st...ealthcare costs are unnecessary or redundant. "Thi...
... Thomas Edison invented the electric light, the ce...,000 patents, but his effort to extract low-grade ...d was dubbed Edisons Folly in the late 1800s. , ,S...ompany he helped to create. Dean Kamen, the creato...0 patents, jokes that his biggest failure is that ...
... GE Healthcare announced today that Rockwood ...ilities in the Spokane, Washington area, has purch...products. , ,One of the new technologies the clini... allow patients to schedule appointments, update t...ically. The patient can take an active role in upl...
...ays low prices. When it comes to consumer goods, A...shop product selection and fanatical commitment to...let going. We like our stuff cheap, and Wal-Mart i...ericans hate to love is getting loads of unfavorab...e successfully derailed its growth plans in certai...
Other Biology Technology:Wisconsin pushes for adoption of electronic health records 2Embracing failure is a necessary step for successful entrepreneurs 2Embracing failure is a necessary step for successful entrepreneurs 3Wal-Mart: How low can you go? 2Wal-Mart: How low can you go? 3Wal-Mart: How low can you go? 4
(Date:10/7/2008)...logy at the University of Minnesota, has been name...r Biology. Tilman will receive a medal, a $100,000...an in a ceremony in Tokyo on Dec. 8. Following the...a scientific symposium. , The award, which is o...receive, is given to one individual in a different...
(Date:10/7/2008)...stitute of Biological Sciences (AIBS) and the Nati...convene a special symposium and workshop for educa...rs (NABT) conference in Memphis, TN. The special ...ach year,s NABT conference, provides classroom tea...ternationally recognized evolutionary biology scho...
(Date:10/7/2008)...s characterized a mechanism that allows for asymme... tracking chromosome movement in live mouse oocyte...t to their vicinity a protein called formin-2. Thi... of the cytoplasm a requirement for embryonic dev...hter cell (called a polar body) resulting from the...
(Date:10/7/2008)...olleagues in France have identified a gene variant...m disorder called sinus node disease. Also known a...ximately one in 600 heart patients older than 65 a...rmanent pacemaker placements in the United States....are, the study provides insight into cellular mech...
Breaking Biology News(10 mins):Minnesota ecology professor wins international award for biodiversity and biofuels research 2Illuminating biology: An evolutionary perspective 2Illuminating biology: An evolutionary perspective 3Illuminating biology: An evolutionary perspective 4Study provides insight on a common heart rhythm disorder 2Study provides insight on a common heart rhythm disorder 3Healthy Interactions and Merck Announce Further Collaboration to Transform Diabetes Education in the U S 21134 1Healthy Interactions and Merck Announce Further Collaboration to Transform Diabetes Education in the U S 21134 2Healthy Interactions and Merck Announce Further Collaboration to Transform Diabetes Education in the U S 21134 3Healthy Interactions and Merck Announce Further Collaboration to Transform Diabetes Education in the U S 21134 4The febit science lounge presents 3A Webinar About Role of Non Protein Coding RNA in Human Genome Set for 11 a m EDT on June 12 21132 1The febit science lounge presents 3A Webinar About Role of Non Protein Coding RNA in Human Genome Set for 11 a m EDT on June 12 21132 2NeurogesX to Present at the Seventh Annual Needham 26 Company Biotechnology and Medical Technology Conference 21130 1NeurogesX to Present at the Seventh Annual Needham 26 Company Biotechnology and Medical Technology Conference 21130 2NeurogesX to Present at the Seventh Annual Needham 26 Company Biotechnology and Medical Technology Conference 21130 3CSCs DynPort Vaccine Company to Continue Plague Vaccine Development 21128 1CSCs DynPort Vaccine Company to Continue Plague Vaccine Development 21128 2CSCs DynPort Vaccine Company to Continue Plague Vaccine Development 21128 3
...ls breathing is fundamental to the treatment of re...automatic rhythmic process that persists without c...t the question that has intrigued many scientists ...ost fail safe vital rhythm throughout life? , Expe...o world renowned scientists Dr. Guyenet from the U...
...in on smaller sections of cell chromosomes than ev...tute have identified nearly 100 chromosome regions...non-small cell lung cancer. The findings provide n...nvolved in the most common type of lung cancer –?a...a range of possible targets for future therapies. ...
...uis is developing new techniques to render a more ...erged on Earth ?with implications for Mars. Carrin...fessor of earth and planetary sciences in Arts &am...al microbes that could lead to provocative conclus...anets. , Blank approaches the task by resolving ph...
... the approval of Baraclude (entecavir) tablets and...tis B in adults. Chronic hepatitis B is a serious ... attacks the liver. The virus can cause lifelong i... cancer, liver failure, and death. According to th...oximately 1.25 million Americans are chronically i...
Other Biology News:How exactly does the brain control breathing? 2High-powered gene profiles provide clues to genes involved in common form of lung cancer 2High-powered gene profiles provide clues to genes involved in common form of lung cancer 3Map of life on Earth could be used on Mars 2Map of life on Earth could be used on Mars 3
...t can be used as a fully functional, stand-alone i...rovides the means to record and tabulate data impo...then be copied and pasted into commercially availa... 123, or into a curve-fitting program. The Data Ma...
This CLS number is a new product number, created to easily match Cornings product number. If showing no availability yet, please order under the old Sigma-Aldrich number (Z71,346-5) or contact custom
...), 2 x 11K will enable researchers to simultaneous...ast genes and identify gene activity during import... sequences representing probes representing 6,256 ...cerevisiae, sourced from the Saccharomyces Genome ...
...tuitive, easy-to-use operating environment for sim... and integration with regulatory compliance requir...sition, re-analysis, custom reports, and data mana.... LCMSsolutions assistant bar provides easy naviga...
Biology Products: