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Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

s obtained in the Bio-Plex assays (see figure). Correlation coefficients (r2) ranged from 0.80 to 0.99 (p<0.001 for all analyses).

The dynamic range of the ELISA used in this study was significantly narrower than that of the Bio-Plex assays. The ELISAs detected 1.6 to 600 pg/ml cytokines, while Bio-Plex assays detected 1.6 to 32,000 pg/ml.


Conclusions
In this study, we were able to demonstrate that testing PBMC samples from asthmatic and healthy children for IL-5, IL-10, IL-13, and IFN-γ by ELISA and Bio-Plex assays yielded similar results. Bio-Plex assays have broader dynamic range and allow for multiplex analysis of cytokines in a single sample. Therefore, Bio-Plex assays have an advantage over the current ELISA methodology when multiple samples have to be analyzed for several cytokines and when available sample volume is limited.


Acknowledgements
We thank Ulrika Bengtsson, Anne-Marie Fornander, Lena Lindell, Ing-Marie Sandberg, and Kristina Warstedt, Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, for excellent technical assistance.

References
Bttcher MF et al., Allergen-induced cytokine secretion in atopic and non-atopic asthmatic children, Pediatr Allergy Immunol, in press

Jenmalm MC et al., Allergen-induced Th1 and Th2 cytokine secretion in relation to specific allergen sensitization and atopic symptoms in children, Clin Exp Allergy 31, 15281535 (2001)

OGarra A and Murphy K, Role of cytokines in determining T-lymphocyte function, Curr Opin Immunol 6, 458466 (1994)

Vignali DA, Multiplexed particle-based flow cy
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