Navigation Links
Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

oplate well (Vignali 2000).

The goal of our study was to compare the Bio-Plex cytokine assays with cytokine ELISA developed in-house for testing cell culture samples from asthmatic and healthy children.


Methods
Cell Isolation and Cultures
Human peripheral blood mononuclear cells (PBMC) were isolated from fresh blood donated by atopic asthmatic children and non-atopic healthy children between the ages of 13 and 15 (Bttcher et al. in press). The cells (1 x 106/ml) were cultured in AIM-V serum-free medium (Life Technologies AB, Sweden) with 20 mM mercaptoethanol (Sigma-Aldrich, Stockholm, Sweden). The cells were stimulated with timothy grass, cat, and dog allergen extracts from ALK (Horsholm, Denmark) at 10,000 standardized units/ml each. The cells were cultured at 37C with 5% CO2 for 144 hr. The supernatants were collected after centrifugation and stored at 70C. Samples were tested simultaneously for cytokines IL-5, IL-10, IL-13, and IFN-γ using both a Bio-Plex human cytokine 4-plex assay and an ELISA made from commercially available antibodies.

Multiplex Assay
A Bio-Plex human cytokine assay for simultaneous quantitation of IL-5, IL-10, IL-13, and IFN-γ was run according to the recommended procedure. In brief, the premixed standards were reconstituted in 0.5 ml of culture medium, generating a stock concentration of 50,000 pg/ml for each cytokine. The standard stock was serially diluted in the same culture medium to generate 8 points for the standard curve. The assay was performed in a 96-well filtration plate supplied with the assay kit. Premixed beads (50 l) coated with target capture antibodi
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
2. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
3. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
4. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
5. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
6. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
8. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
9. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
10. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
11. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
Post Your Comments:
(Date:4/23/2015)... April 23, 2015 Proove ... Personalized Medicine, is excited to announce the launch ... . This profile assesses 62 genetic variations to ... information for commonly prescribed non-opioid pain medications, namely ... Neurontin), alprazolam (brand name Xanax) and acetaminophen (brand ...
(Date:4/23/2015)... NY , April 23, 2015 /PRNewswire/ - Crystal ... an Executive Informational Overview ® (EIO) on Aeterna ... 52-page report is available on Crystal Research Associates, website ... on Aeterna Zentaris, website at www.aezsinc.com . ... into a specialty biopharmaceutical company in oncology, endocrinology, and ...
(Date:4/22/2015)... 23, 2015  The Heinz Family Foundation today ... Heinz Awards, which will present unrestricted cash awards ... their creativity and determination in finding solutions to ... This year,s recipients include a prolific cartoonist whose ... and personal relationships; two former Marines who are ...
(Date:4/22/2015)... 2015   MPIRICA Health Inc. , a ... Series A financing from the McQuinn Trust, an ... consumer and employer awareness of MPIRICA.com, delivering easily ... 4,800 U.S. hospitals. "As a patient ... concerned about choosing the hospital and surgeon most ...
Breaking Biology Technology:Proove Biosciences Launches World’s First Test to Predict Responders to Commonly Prescribed Non-Opioid Pain Medications 2Proove Biosciences Launches World’s First Test to Predict Responders to Commonly Prescribed Non-Opioid Pain Medications 3Crystal Research Associates, LLC Issues Executive Informational Overview (EIO) on Aeterna Zentaris Inc. 2Crystal Research Associates, LLC Issues Executive Informational Overview (EIO) on Aeterna Zentaris Inc. 3From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 2From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 3From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 4From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 5From a Scientist Who Develops Human Microlivers to a New Yorker Cartoonist Who Masterfully Depicts the Humor and Heartbreak of Everyday Life: The 20th Heinz Awards Honors Six Changemakers 6MPIRICA Health Inc. Secures $1.6M to Promote FICO-Like Quality Scores for Surgeries 2
... capable of some pretty remarkable things when they,re placed ... can even produce electricity. Driven by the vision ... team of University of Houston scientists has set out ... known as piezoelectrics, which naturally produce electricity when literally ...
... , , , ... Inc. (NYSE: BEC ) announced today that its Board of ... of outstanding common stock, payable on August 24, 2009 to all stockholders ... consecutive, quarterly payout of dividends. , , (Logo: ...
... , , ... Amgen (Nasdaq: AMGN ) and GlaxoSmithKline (GSK) today announced ... monoclonal antibody denosumab for postmenopausal osteoporosis (PMO) in Europe, Australia, New ... Amgen will commercialize the drug for PMO and oncology in ...
Cached Biology Technology:Charging ahead: University of Houston team revealing secrets of electricity-producing materials 2Charging ahead: University of Houston team revealing secrets of electricity-producing materials 3Amgen to Collaborate With GlaxoSmithKline to Commercialize Denosumab in Europe for Postmenopausal Osteoporosis (PMO) 2Amgen to Collaborate With GlaxoSmithKline to Commercialize Denosumab in Europe for Postmenopausal Osteoporosis (PMO) 3Amgen to Collaborate With GlaxoSmithKline to Commercialize Denosumab in Europe for Postmenopausal Osteoporosis (PMO) 4Amgen to Collaborate With GlaxoSmithKline to Commercialize Denosumab in Europe for Postmenopausal Osteoporosis (PMO) 5Amgen to Collaborate With GlaxoSmithKline to Commercialize Denosumab in Europe for Postmenopausal Osteoporosis (PMO) 6Amgen to Collaborate With GlaxoSmithKline to Commercialize Denosumab in Europe for Postmenopausal Osteoporosis (PMO) 7
(Date:4/1/2015)... Conn. , Apr. 1, 2015  NXT-ID, Inc. ... biometric authentication company focused on the growing mobile commerce ... of Wocket smart wallets is underway to early access ... access group includes usage at retail outlets including Walmart, ... Pharmacy. Users report Wocket was accepted at all outlets ...
(Date:3/31/2015)... YORK , March 31, 2015   Guidepoint ... the launch of the Post-Surgical Pain Management TRACKER, part ... products tracking usage trends in the medical device and ... access to data including treatment volumes, market share, and ... the pain associated with joint surgery. The ...
(Date:3/24/2015)... Mar. 24, 2015 Research and Markets ... "Global Iris Recognition Market 2015-2019" report to ... Iris Recognition market to grow at a CAGR of ... report covers the present scenario and the growth prospects ... 2015-2019. To calculate the market size, the report considers ...
Breaking Biology News(10 mins):NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 2NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 3NXT-ID Reports Second Shipment of Wocket Smart Wallets Underway to Early Access Pre-order Customers and Provides User Feedback 4Guidepoint Launches Post-Surgical Pain Management TRACKER 2Guidepoint Launches Post-Surgical Pain Management TRACKER 3Global Iris Recognition Market 2015-2019 with 3M Cogent, Bioenable Technologies, Cross Match Technology, Iris ID Systems & Safran Dominating 2
... ARTICLE #1 FOR IMMEDIATE RELEASE Evidence that inexpensive ... Energy & Fuels Amid sticker-shock fuel prices, researchers ... tests verifying that a simple, inexpensive device attached to a ... to 20 percent. That translates into several more precious miles ...
... They are used in cancer treatments, automobile sensors, ... production. However, until recently, scientists couldn,t create the ... impacts on the environment. A new method, created ... only eliminates any negative environmental impact, but also ...
... and his collaborators have developed a way to mass-produce ... less expensive. Metcalf set out to ... as phosphonates, is made in nature by bacteria. He ... have antibiotic properties. Recently, Metcalf and his lab successfully ...
Cached Biology News:American Chemical Society's Weekly PressPac -- Sept. 24, 2008 2American Chemical Society's Weekly PressPac -- Sept. 24, 2008 3American Chemical Society's Weekly PressPac -- Sept. 24, 2008 4American Chemical Society's Weekly PressPac -- Sept. 24, 2008 5American Chemical Society's Weekly PressPac -- Sept. 24, 2008 6American Chemical Society's Weekly PressPac -- Sept. 24, 2008 7American Chemical Society's Weekly PressPac -- Sept. 24, 2008 8MU scientists go green with gold, distribute environmentally friendly nanoparticles 2New way to make malaria medicine also first step in finding new antibiotics 2New way to make malaria medicine also first step in finding new antibiotics 3New way to make malaria medicine also first step in finding new antibiotics 4
... Biotin label transfer is fast emerging ... interaction discovery and confirmation. Until now, only ... targeted with label transfer reagents such as ... chain analog, Mts-Atf-LC-Biotin, are exciting additions to ...
... of 3 siRNAs specifically targeted to your ... product. The siTrio reagent guarantees you get ... gene when used under standard conditions (100 ... optimal transfection with one of B-Bridges controls). ...
... a compact benchtop and stackable multi-function ... with a large display screen to ... Air circulation inside to evenly distribute ... be easily calibrated from the microprocessor ...
...
Biology Products: