Navigation Links
Bio-Plex Cytokine Immunoassays and ELISA: Comparison of Two Methodologies in Testing Samples From Asthmatic and Healthy Children, Rev A

Maria C Jenmalm,1 Michele Malit,2 Jim Torrence,2 and Aiguo Zhang,2
1
Department of Molecular and Clinical Medicine, Division of Paediatrics, Linkping University, Sweden, and 2 Bio-Rad Laboratories, Inc., 2000 Alfred Nobel Drive, Hercules, CA 94547 USA


Introduction
Cytokines play a significant role in hematopoiesis, inflammation, and wound healing. These important immunoregulators mediate interactions between various cells, and their dysregulated production may contribute to disease pathogenesis. Elevated levels of cytokines may indicate inflammation or disease progression. Although cytokine functions are complex, cytokine profiles are highly relevant parameters of an immune response. Different cytokines possess biologically overlapping functions, and they have the ability to regulate production of other cytokines. Therefore, analysis of the function of the complete set of cytokines expressed within microenvironments (e.g., a site of inflammation) is often of more value than analysis of a single isolated cytokine (OGarra and Murphy 1994).

A number of methodologies can be used to measure cytokines. The most common one is the enzyme-linked immunosorbent assay (ELISA), which allows measurement of different cytokines with high sensitivity and specificity. This technology, however, is restricted to analyzing one cytokine target at a time, which limits its usefulness when simultaneous measurement of multiple cytokine targets is desired. The new suspension bead array technology employed in the Bio-Plex system (Luminex xMAP technology) allows multiple cytokine targets to be measured from a single sample in a micr
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
2. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
3. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
4. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
5. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
6. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
8. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
9. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
10. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
11. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
Post Your Comments:
(Date:9/2/2014)... MA (PRWEB) September 02, 2014 ... today the signage of a distribution agreement with ... is now distributing Myriant’s bio-succinic acid in the ... and Ireland, targeting customers in the industrial and ... distribution powerhouse, Azelis offers a far-reaching chemical portfolio ...
(Date:9/2/2014)... Inc. (Nasdaq: ONTY ) announced today that ... present at the Rodman & Renshaw 16 th Annual ... on Tuesday, Sept. 9, 2014 at 2:05 p.m. Eastern. ... will be accessible by visiting the Oncothyreon website www.oncothyreon.com ... About Oncothyreon Oncothyreon is a biotechnology company ...
(Date:9/2/2014)... 2014  Spherix Incorporated (SPEX) -- an intellectual property development company ... today announced that the United States Patent & Trademark ... the month of August that are part of a ... issued patents are: , U.S.RE45,065 issued ... 2014; and , U.S.RE45,095 issued August 26, 2014.  ...
(Date:9/1/2014)... The global oligonucleotide synthesis market is expected ... million in 2014, growing at a CAGR of ... synthesis market is categorized on the basis of ... The synthesized oligonucleotides segment is expected to register ... market during the forecast period, owing to an ...
Breaking Biology Technology:Myriant and Azelis Sign European Distribution Agreement for Bio-Succinic Acid 2United States Patent & Trademark Office Issues Three New Standard Essential Patents to Spherix 29.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 29.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 39.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 49.8% CAGR for Oligonucleotide Synthesis Market Globally to 2019 Says a New Research Report Available at RnRMarketResearch.com 5
... Mass., Sept. 19 Boston Scientific,Corporation (NYSE: BSX ... Life,Sciences Conference, which is being held September 22 - ... Chief Executive Officer, will make a,30-minute presentation which will ... Thursday, September 25, beginning at approximately 9:00,a.m. ET., ...
... PARK, Ill., Sept. 19 Abbott (NYSE:,ABT) will announce ... before the market opens., The announcement will be ... at 8 a.m. Central time (9 a.m. Eastern), and ... http://www.abbottinvestor.com . An archived edition of the call will ...
... China, Sept. 19 /Xinhua-PRNewswire-FirstCall/ --,China-Biotics, Inc. (OTC ... Chinese firm specializing in the manufacture,research, development, ... that at its 2008 Annual General Meeting ... the Company,s nominees to the,Board of Directors., ...
Cached Biology Technology:China-Biotics, Inc. Announces Results of 2008 Annual General Meeting of Shareholders 2
(Date:9/2/2014)... distribution of media will have lower carbon emissions ... file sizes are large. , That,s the conclusion ... of Industrial Ecology that looked at the ... PlayStation3. Researchers found that Blu-ray Discs delivered via ... game files downloaded over broadband Internet. For their ...
(Date:9/2/2014)... New research suggests that professional baseball pitchers with poor core ... in a single season because of injury than are pitchers ... and pelvis. , In the study, 347 pitchers were assessed ... in their pelvis as they raised a leg to step ... at least 30 days cumulative, not consecutive during ...
(Date:9/2/2014)... new study published in the September issue of ... identifies a novel strategy to diagnose the leading ... irreversible structural damage has occurred. This advance involves ... on the endothelium of retinal vessels. Using new ... distinguish the early molecular development of diabetic retinopathy. ...
Breaking Biology News(10 mins):In pro baseball pitchers, weak core linked to more missed days 2In pro baseball pitchers, weak core linked to more missed days 3Molecular probes permit doctors to detect diabetic retinopathy before vision fails 2
... for hundreds of years as an herbal remedy to prevent ... commonly used herbal medicines in North America. However, in spite ... on the body,s immune system are conflicting. ... University Indianapolis are investigating the possibility that Echinacea has other ...
... 20 million people in the U.S., and many more worldwide, ... developing mesothelioma, a malignant cancer of the membranes that cover ... Moreover, asbestos exposure increases the risk of lung cancer among ... understand why asbestos causes cancer. The answer ...
... Tuesday 29 June 2010, announces this year,s award winners ... to theoretical physicist Professor Edward Witten for outstanding, transformative ... influential physicists of the past 30 years, he has ... particle physics and cosmology to theoretical areas of string ...
Cached Biology News:Mystery unraveled: How asbestos causes cancer 2IOP announces 2010 award winners 2