| HOME >> BIOLOGY >> TECHNOLOGY |
Maria C Jenmalm,1 Michele Malit,2 Jim Torrence,2
and Aiguo Zhang,2
1 Department of Molecular and Clinical Medicine, Division of Paediatrics,
Linkping University, Sweden, and 2 Bio-Rad Laboratories, Inc., 2000 Alfred
Nobel Drive, Hercules, CA 94547 USA
Introduction
Cytokines play a significant role in hematopoiesis, inflammation, and
wound healing. These important immunoregulators mediate interactions between
various cells, and their dysregulated production may contribute to disease
pathogenesis. Elevated levels of cytokines may indicate inflammation or
disease progression. Although cytokine functions are complex, cytokine
profiles are highly relevant parameters of an immune response. Different
cytokines possess biologically overlapping functions, and they have the
ability to regulate production of other cytokines. Therefore, analysis
of the function of the complete set of cytokines expressed within microenvironments
(e.g., a site of inflammation) is often of more value than analysis of
a single isolated cytokine (OGarra and Murphy 1994).
A number of methodologies can be used to measure cytokines. The most
common one is the enzyme-linked immunosorbent assay (ELISA), which allows
measurement of different cytokines with high sensitivity and specificity.
This technology, however, is restricted to analyzing one cytokine target
at a time, which limits its usefulness when simultaneous measurement of
multiple cytokine targets is desired. The new suspension bead array technology
employed in the Bio-Plex system (Luminex xMAP technology) allows multiple
cytokine targets to be measured from a single sample in a micr
'"/>
Source:
Related biology technology :
1. Detection of Phosphorylated and Total ERK and p38 MAPK by Bio-Plex Assay, ELISA, and Western Blotting, Rev A
2. Correlation of Bio-Plex Suspension Array Reader Validation With Multiplex Cytokine Assay Performance, Rev A
3. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
4. Application of the Bio-Plex Cell Lysis Kit in Western Blot Analysis of Phosphoproteins, Rev A
5. Development of a Multiplex Bead-Based Assay for Antibody Screening of a Nonhuman Primate Colony on the Bio-Plex System
6. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
7. Simultaneous Detection of 8 Cytokines in Mouse or Human Sera
8. Principles of Curve Fitting for Multiplex Sandwich Immunoassays, Rev B
9. Mix-and-Read, Bead- and Cell-Based Immunoassays Using the FMAT 8100 HTS System
10. Quantitative Measurement of Cell Proliferation Using the BrdU ELISA: A Comparison Between Colorimetric and Chemiluminescent Detection
11. PCR Performance Comparisons Between pfuturbo and Taq DNA Polymerases
| Copyright © 2003-2012 Bio-Medicine. All rights reserved. ABOUT | CONTACT US | DISCLAIMER | PRIVACY POLICY | TERMS AND CONDITIONS |  |