Navigation Links
Big Blue Rodent Cell Cultures for Mutagenesis Studies

Rat and mouse embryonic cell lines are useful for in vitro mutagenesis studies

Denise L. Wyborski
Stratagene Cloning Systems, Inc.

The Big Blue rat cell line and the Big Blue transgenic mouse embryonic cell line are useful for in vitro mutagenesis studies. These cell lines are part of the Big Blue transgenic rodent mutagenesis assay system,* a system for detecting mutations from several known mutagens in a variety of tissues from transgenic mice and rats.1-4

figure 1

The Big Blue rat cell line is derived from a Rat2 embryonic fibroblast cell line transfected with the Big Blue lambda shuttle vector and the pSV2Neo plasmid, which provides an antibiotic-selection marker.5-7 The Big Blue lambda shuttle vector (figure 1) is 45 kb in size and contains the lacI and alphalacZ genes. Each cell contains 50 to 70 copies of the lambda shuttle vector integrated into the genome at two sites. The Big Blue rat cell line is resistant to the antibiotic G418 and is a thymidine kinase mutant; the chromosome number is polyploid. The spontaneous mutant frequency of this cell line is approximately 3.7 x 105.

The Big Blue transgenic mouse embryonic cell line is derived from embryonic cells of transgenic mice containing the Big Blue lambda shuttle vector. The cell line was immortalized by chemical transformation and differentiation and has survived more than 50 generations. Each cell contains at least 40 copies of the Big Blue lambda shuttle vector integrated into the genome of the cell at the identical location as in the genome of the transgenic mice. This cell line exhibits a spontaneous mutant frequency of approximately 9.28 x 105.

The Big Blue cell lines use the lac
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Detection of Reporter Gene Activity in Cell Cultures and Murine Epidermis After Helios Gene Gun-Mediated Particle Bombardment
2. High-Efficiency Site-Directed Mutagenesis of Large Plasmid Vectors
3. Randomize Gene Sequences with New PCR Mutagenesis Kit
4. In-Vitro Metabolism Studies Using Data-Dependent LC/MSn
5. Precursor miRNAs for Successful miRNA Functional Studies
6. DGGE Analysis for Non-Invasive Studies of Primate Diet
7. Using the DCode System to Identify DNA Sequence Variation for Studies of Population Structure in Marine Organisms
8. SNPs Powerful Tools for Association Studies
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Big Blue Rodent Cell Cultures for Mutagenesis Studies

(Date:4/2/2015)... 02, 2015 InGaAs PIN photo diodes ... sensitivity and high-speed response. Marktech introduces the newest series ... can with active areas of 0.1mm, 0.3mm and 0.8mm. ... and high speed fiber modules. , Standard spectral range ... These parts offer considerable speed improvements over the current ...
(Date:4/1/2015)... , April 1, 2015  Adaptive Biotechnologies ... The Lancet Oncology showing that ... company,s next-generation sequencing-based minimal residual disease (MRD) detection ... MRD test) accurately identifies patients with diffuse large ... relapse after initial treatment, usually several months before ...
(Date:4/1/2015)... -- Emerson Ecologics is excited to announce its first ... . The conference will provide integrative healthcare practitioners with ... fulfilling practice. Scheduled for November 13-15, ... the conference will be a one-of-a-kind, "get it done" ... and leave with an action plan in hand. Highlights ...
(Date:4/1/2015)... , April 1, 2015  ValGenesis Inc., the market ... today that it has completed its Service Organization Controls ... Engagements (AICPA, Professional Standards) for the period of January ... 2014.  ... The SOC 2 examination ...
Breaking Biology Technology:Marktech Optoelectronics Announces High Speed InGaAs PIN Diode 1.7µm 2Adaptive Biotechnologies' clonoSEQ Minimal Residual Disease Test Predicts Relapse in Diffuse Large B-Cell Lymphoma Patients by Sensitively Detecting Circulating Tumor DNA 2Adaptive Biotechnologies' clonoSEQ Minimal Residual Disease Test Predicts Relapse in Diffuse Large B-Cell Lymphoma Patients by Sensitively Detecting Circulating Tumor DNA 3Adaptive Biotechnologies' clonoSEQ Minimal Residual Disease Test Predicts Relapse in Diffuse Large B-Cell Lymphoma Patients by Sensitively Detecting Circulating Tumor DNA 4Adaptive Biotechnologies' clonoSEQ Minimal Residual Disease Test Predicts Relapse in Diffuse Large B-Cell Lymphoma Patients by Sensitively Detecting Circulating Tumor DNA 5Emerson Ecologics Announces First Annual Conference for Integrative Healthcare Practitioners 2Emerson Ecologics Announces First Annual Conference for Integrative Healthcare Practitioners 3ValGenesis Inc. Successfully Completes Service Organization Controls 2 (SOC 2) Report under AT Section 101 2ValGenesis Inc. Successfully Completes Service Organization Controls 2 (SOC 2) Report under AT Section 101 3
... technology in the field of electrospinning for applications primarily ... 16 Zeus, Inc. announces a new technology platform ... polymer fibers with thicknesses ranging from the nano to ... shapes from solutions, pastes, and bioabsorbables. The result is ...
... crisis? Probably not, but a hamster wearing a ... provide a new and renewable source of electricity. , ... researchers have also generated electrical current from a tapping ... phones and other handhelds one step closer to powering ...
... NEW YORK, Feb. 13 On February 10, 2009, ... notice from the NYSE Alternext US (the "Alternext") indicating ... 704 of the Alternext Company Guide (the "Guide"), which ... shareholders annually. On November 3, 2008, the Company had ...
Cached Biology Technology:Electrospinning: Emerging New Technology at Zeus 2Nanogenerators produce electricity from running rodents and tapping fingers 2Nanogenerators produce electricity from running rodents and tapping fingers 3NeoStem Receives Notice of Noncompliance from NYSE Alternext US 2
(Date:3/20/2015)... Conn. , March 20, 2015 NXT-ID, ... a biometric authentication company focused on the growing mobile ... smart wallet is one of the products featured in ... March 20th at 11:00 PM EST on the DIY ... to Las Vegas , site of ...
(Date:3/17/2015)... 2015 Emotient, the leader in facial ... general availability of Emotient Analytics , the ... of facial expressions. The system analyzes videos of ... and services. It delivers audience response metrics - ... as derived from facial evidence of emotional states. ...
(Date:3/12/2015)... BERLIN , March 12, 2015 ... IAM, a standardized identity and access management (IAM) ... reliable implementation schedule. With Beta System,s new IAM ... and best practices gleaned from multiple IAM implementations ... includes the software as well as any necessary ...
Breaking Biology News(10 mins):NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 2NXT-ID's Wocket Biometric Smart Wallet to Air on "I Want That" International CES Special 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 2New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 3New Emotient Web Service Measures Audience Response Using On-Demand Facial Expression Analysis 4Beta Systems Fast Forward IAM Available Now: a Standardized IAM Package at a Fixed Price 2
... Zeroing in on the core cellular mechanisms of sleep, ... for the first time a single gene mutation that ... fruit flies sleep. , In its normal state, the ... channel that controls the flow of potassium into cells, ...
... you're well aware that hair follicles are expendable. The product ... le chat nu, would quickly succumb in the wild—its winter ... down the midback and tail—and needs special care to thrive ... other hand, can be very instructive. Understanding how hair develops ...
... in the X chromosomes of women has revealed an ... work by researchers at the Duke University Institute for ... , The results may have important implications for understanding ... and females, in terms of both health and disease, ...
Cached Biology News:Wisconsin researchers identify sleep gene 2Wisconsin researchers identify sleep gene 3Wisconsin researchers identify sleep gene 4Hirsute Or Hairless? Two Proteins May Spell The Difference 2Hirsute Or Hairless? Two Proteins May Spell The Difference 3Variation in women's X chromosomes may explain differences among individuals, between sexes 2Variation in women's X chromosomes may explain differences among individuals, between sexes 3
Tris-Hepes kit of BG-163 & BG-165 (order number BG-166)...
... electronic from BRAND adds ergonomics and ... the only electronic, motor-driven repeating pipette ... including BRAND PD-Tip syringe tips, Eppendorf ... Tips,and VWRbrand Combi-Syringes. Select virtually any ...
Incorporating the innovative InGaAs detection system the Cary 6000i is the worlds most advanced UV-Vis-NIR spectrophotometer delivering superior signal to noise performance and outstanding sensitivit...
... coated microplates can be used for efficient, ... fusion proteins for ELISA or other assays ... such proteins are bound with high efficiency ... left) and will not elute during the ...
Biology Products: