Navigation Links
Better RNA Amplification

ect incorporation of dye coupled NTPs is inefficient and results in low yields and low specific activity aRNA. This can make the cost of producing aRNA by direct incorporation very high. In addition, incorporation efficiency varies for different labels (e.g. Cy3 NTP vs Cy5 NTP). These problems can be avoided by incorporating an amino allyl NTP into the aRNA and subsequently coupling its reactive amino group with an NHS ester labeled dye (e.g. Cy or other dye). Unlike dye coupled NTPs, amino allyl modified NTPs are incorporated almost as efficiently as unmodified NTPs and are much less expensive than the dye coupled NTPs.

In the Ambion Amino Allyl MessageAmp II protocols, amino allyl UTP is incorporated during the transcription step to produce amino allyl modified aRNA. After a quick purification step, the aRNA is ready for coupling to the NHS ester label of your choice (a variety of inexpensive NHS ester dyes and other nonisotopic labels are available from Amersham Biosciences, Molecular Probes, and Pierce). After dye coupling the reaction is quenched and a final purification is performed, yielding labeled aRNA ready for hybridization.


back to top


Ordering Information Cat# Product Name Size 1751 MessageAmp II aRNA Amplification Kit 20 rxns 1753 Amino Allyl MessageAmp II aRNA Amplification Kit
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Better Way to Isolate RNA AND Protein From the Same Sample
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better RNA Amplification

(Date:12/24/2014)... (PRWEB) December 23, 2014 Earlier this ... James L. Sherley, director of the Adult Stem Cell ... an often overlooked and under appreciated unique property of ... by Distributed Stem Cells: Misunderstood in the Past, Important ... to congress participants. He gave the address at ...
(Date:12/24/2014)... Island, New York (PRWEB) December 23, 2014 ... and the Long Island Affiliate of ITRA Global, the national ... slow but steady recovery. This is evidenced by the ... the strong stock market. Low energy costs have held ... The only negative is the housing market remaining soft. ...
(Date:12/24/2014)... report expects global cell isolation and expansion market to ... carefully analyzed data about the vital drivers, restraints, trends, ... Copy of Report @ http://bit.ly/1yUxy0T , According ... will keep witnessing growth at an impressive CAGR during ... by rapid technological advancements, government funding and investments, and ...
(Date:12/24/2014)... Texas , Dec. 23, 2014 ... company focused on gynecologic disease, announced today that ... Schuler , Birchview Fund LLC and several Vermillion ... of unregistered shares of Vermillion,s common stock and ... stock in a private placement.  ...
Breaking Biology Technology:Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 2Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 3Adult Stem Cell Technology Center, LLC’s Director Sherley's Address on What’s Holding Back Regenerative Medicine Featured on Omics Group Website 4ITRA Global Reports Long Island Is Out of Sync With The National Office Market 2ITRA Global Reports Long Island Is Out of Sync With The National Office Market 3The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 2The Cell Expansion Market is Expected to Reach $14.8 billion by 2019 - New Research Report by MarketsandMarkets 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 2Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 3Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 4Vermillion Announces Equity Financing of up to $18.9 Million; Suspends ATM Program 5
... Jan. 11, 2011 Reportlinker.com announces that ... in its catalogue: Sample ... (Results of The Kalorama Survey of Labs) ... Sample preparation and library preparation are ...
... of Chemistry Dunwei Wang has received a career award ... energy technology research and expand the community outreach programs ... on developing novel technologies for clean energy applications, has ... a flexible webbing of nano-scale wires that has shown ...
... N.C., Jan. 10, 2011 Takeda Pharmaceutical Company ... they have entered into an exclusive, worldwide licensing ... for the risk of Alzheimer,s disease in high-risk ... study pioglitazone in connection with the TOMM40 biomarker ...
Cached Biology Technology:Reportlinker Adds Sample Preparation and Library Preparation for DNA Sequencers (Results of The Kalorama Survey of Labs) 2Reportlinker Adds Sample Preparation and Library Preparation for DNA Sequencers (Results of The Kalorama Survey of Labs) 3Developer of 'nanonets' snares National Science Foundation Career Award 2Takeda and Zinfandel Pharmaceuticals Sign Licensing Agreement for Alzheimer's Disease Biomarker in Combination with Pioglitazone 2
(Date:12/3/2014)... 2014   Marvin Test Solutions , a ... for military, aerospace, and manufacturing organizations, today announced ... TS-900 PXI semiconductor test platform . The ... systems to customers at a fraction of the ... ATE. "Our semiconductor customers asked ...
(Date:11/21/2014)... , Nov. 19, 2014  Earlier this year ... York College, and one of the most prolific inventors ... that are transmitted from Smartphones to third party agencies. ... and has one of the earliest known patents in ... usage in the military, child care, elder care and ...
(Date:11/21/2014)... JOSE, Calif. , Nov. 20, 2014   ... global leader in microcontroller (MCU) and touch technology solutions, ... digital temperature sensors with the widest V cc ... delivers higher temperature accuracy and faster I 2 C ... registers and serial EEPROM memory making them ideal for ...
Breaking Biology News(10 mins):Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 2Marvin Test Solutions Brings New Capabilities to PXI-Based Semiconductor Test with TS-960 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 2Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 3Atmel Launches Industry's First Wide-V(cc) Low-Power Temperature Sensor Family 4
... a majority of hip fracture patients of all ages and ... a new study presented today at the 2013 Annual Meeting ... whether or not living in a warm, sunny climate improves ... vitamin D levels of 1,539 patients, including 448 acute hip ...
... environmental engineer has received a three-year, $561,730 grant to identify ... water providers can take action to contain and curb the ... Foundation have awarded Assistant Professor Hyeok Choi the grant to ... exist so that the harmful algae can be monitored. , ...
... Some diseases are caused by single gene mutations. Current ... produce hundreds of potential gene candidates, making it difficult ... a team of researchers led by Rockefeller University scientists ... the hunt for disease-causing genes. The investigation, spearheaded ...
Cached Biology News:UT Arlington engineer to search for bad algal blooms 2Researchers create map of 'shortcuts' between all human genes 2Researchers create map of 'shortcuts' between all human genes 3
E1A (adenovirus early region 1)...
... 1 ( Abpromise for all tested ... peptide: MAATGANAEK AESHNDCPVR LLNPNIAKMK EDILYHFNLT TSRHNFPALF GDVKFVCVGG ... acids 1-99 of Human Uridine Phosphorylase 1 ... 7378 Swiss Protein ID: ...
Nucleobindin 1 [NUCB1]...
Presenilin-1, N-terminus....
Biology Products: