Navigation Links
Better RNA Amplification

. RNA samples were amplified and labeled using both procedures and hybridized to GeneChip Human Genome Focus Arrays following the Affymetrix guidelines. Microarray expression profiling data comparing MessageAmp II to the Affymetrix recommended protocol for GeneChip analysis indicates a high level of concordance between the two procedures (Figure 2).

Figure 2. Replicate Analysis of Two Amplification Protocols. Normalized array results are plotted from replicate amplifications. Both MessageAmp II (top) and Affymetrix standard protocol (bottom) were used to amplify identical total RNA samples (1 g), and equivalent amounts of the resulting aRNA were hybridized on matched lot Human Genome Focus arrays. IVT reactions were performed for 4 hr. Correlation values are included and indicate a measure of reproducibility with both methods.


Complete Kits for Amplifying RNA and Amino Allyl Labeling

The MessageAmp II and Amino Allyl MessageAmp II aRNA Amplification Kits contain all the necessary reagents for first-strand cDNA synthesis, RNase H digestion, second-strand cDNA synthesis, cDNA purification, in vitro transcription, and aRNA purification. The Amino Allyl MessageAmp II Kit also contains reagents for amino allyl labeling, dye coupling, and labeled aRNA clean-up. Reagents for 20 amplification reactions and a detailed Instruction Manual are included with both kits.

MessageAmp II is covered by patents exclusively licensed to Incyte Genomics.


* Incorporating Amino Allyl UTP during Amplification
Dir
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Better Way to Isolate RNA AND Protein From the Same Sample
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better RNA Amplification

(Date:5/23/2013)... Newmarket, NH (PRWEB) May 23, 2013 Ralph ... all of their emergency management products and emergency survival ... , This announcement was made at the quarterly executive ... meeting with a moment of silence for the Oklahoma tornado ... executives that the quality of their emergency kits is paramount. ...
(Date:5/22/2013)... Mechanicsburg, PA (PRWEB) May 22, 2013 ... Controller with the integrated potentiometers or via a ... compact design measuring two and one half inches square ... from a zero voltage switched, low noise solid state ... line power isolation for the communication port and sensor ...
(Date:5/22/2013)... Washington, NY (PRWEB) May 22, 2013 ... comprehensive line of accessories for the mobile lifestyle, announces ... charge, sync, protect, and manage multiple iPad devices efficiently. ... award and the 2012 Good Design award, MultiCharger-X is ... the iPad, wherever it is deployed. , ...
(Date:5/22/2013)... New York (PRWEB) May 22, 2013 ... the 3rd Annual Disruptive Innovations to Advance Clinical Trials ... Copley Plaza Hotel in Boston, MA. , Disruptive ... Janssen Pharmaceuticals and more share bold approaches to reduce ... , “Disruptive innovation needs to be big, or ...
Breaking Biology Technology:PHI Emergency Management on Announces Father's Day Specials All Emergency Survival Kits 2New AC Temperature Controller Announced by Oven Industries 2iLuv Now Shipping the All New MultiCharger-X 2iLuv Now Shipping the All New MultiCharger-X 3Disruptive Innovation Hits Clinical Trials, Dedicated Event Launches 2
... Sometimes, computer hard drives just go bad. Not so often, ... too. Such was the case beginning at 11:57 p.m. Monday ... failure that ricocheted systemwide and downed dial-up and DSL users ... She says service was restored at 2:40 a.m. to all ...
... Bratton is determined to give heavy email users something they lack: ... , and a heavy email user, talks about the inspiration for ... the wireless world. , , WTN: How much email do you ... around 1,000 messages a day from various email accounts with about ...
... conference, scheduled for June 3-4 in Milwaukee, is being ... its subsidiary, the Wisconsin Innovation Network . Organizers ... conference by giving entrepreneurs and other representatives of emerging ... big companies would finance - if they only could ...
Cached Biology Technology:TDS Suffers 29-State System Outage 2TDS Suffers 29-State System Outage 3WTN Interview: Alex Bratton: EmailRX's founder on viruses and spam 2WTN Interview: Alex Bratton: EmailRX's founder on viruses and spam 3Connecting Investment Capital with Entrepreneurs: Investors to lay out what theyre looking for in startup companies 2
(Date:5/22/2013)... major new effort to educate the public and decision ... clean water that looms ahead in the 21st century. ... current edition of Chemical & Engineering News , ... the world,s largest scientific society. , Bassam Z. Shakhashiri, ... Ph.D., explain that shortages of reliable supplies of fresh ...
(Date:5/22/2013)... changes in cellular DNA can endanger the whole organism, ... Researchers at Ludwig-Maximilians-Universitaet (LMU) in Munich now report how ... double helix. , The DNA in our cells controls ... our bodies. The instructions for this are encoded in ... DNA, the bases adenine (A), cytosine (C), guanine (G) ...
(Date:5/22/2013)... biodiversity of pollinating insects and wild plants have slowed ... Researchers led by the University of Leeds and the ... of dramatic reductions in the diversity of species in ... 1980s. , But the picture brightened markedly after 1990, ... among bees, hoverflies and wild plants. , ...
Breaking Biology News(10 mins):DNA damage: The dark side of respiration 2Encouraging signs for bee biodiversity 2Encouraging signs for bee biodiversity 3
... in the developing world heat and cook with a ... people especially women to large doses of ... lung disease. In a study just published online ... researchers at the University of Wisconsin-Madison have associated indoor ...
... This release is available in German . ... able to associate and store the name and face of a ... meet that person. In this example, the recall of the information ... the brain remains one of the great mysteries of modern neurobiology. ...
... mutation in plants that was selected twice - during both ... as the source of variation in the evolution of fruit ... of key changes during the domestication of rice. "We ... man-made changes was the same," said one of the authors ...
Cached Biology News:Indoor air pollution linked to cardiovascular risk 2Indoor air pollution linked to cardiovascular risk 3How memory is read out in the fly brain 2How memory is read out in the fly brain 3Evolution and domestication of seed structure shown to use same genetic mutation 2
...
Alexa Fluor 647 anti-mouse Qa-2...
Mouse monoclonal [0.T.81] to Lac1 ( Abpromise for all tested applications). Antigen: Recombinant TrpE fusion protein containing full length E. coli Lac1....
Peptide-affinity Purified Polyclonal Antibody to ICEBERG Peptide with sequence EEDPQLASKMGLH, from C Terminus of the protein sequence according to NP_067546...
Biology Products: