Navigation Links
Better RNA Amplification

. RNA samples were amplified and labeled using both procedures and hybridized to GeneChip Human Genome Focus Arrays following the Affymetrix guidelines. Microarray expression profiling data comparing MessageAmp II to the Affymetrix recommended protocol for GeneChip analysis indicates a high level of concordance between the two procedures (Figure 2).

Figure 2. Replicate Analysis of Two Amplification Protocols. Normalized array results are plotted from replicate amplifications. Both MessageAmp II (top) and Affymetrix standard protocol (bottom) were used to amplify identical total RNA samples (1 g), and equivalent amounts of the resulting aRNA were hybridized on matched lot Human Genome Focus arrays. IVT reactions were performed for 4 hr. Correlation values are included and indicate a measure of reproducibility with both methods.


Complete Kits for Amplifying RNA and Amino Allyl Labeling

The MessageAmp II and Amino Allyl MessageAmp II aRNA Amplification Kits contain all the necessary reagents for first-strand cDNA synthesis, RNase H digestion, second-strand cDNA synthesis, cDNA purification, in vitro transcription, and aRNA purification. The Amino Allyl MessageAmp II Kit also contains reagents for amino allyl labeling, dye coupling, and labeled aRNA clean-up. Reagents for 20 amplification reactions and a detailed Instruction Manual are included with both kits.

MessageAmp II is covered by patents exclusively licensed to Incyte Genomics.


* Incorporating Amino Allyl UTP during Amplification
Dir
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Better Way to Isolate RNA AND Protein From the Same Sample
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better RNA Amplification

(Date:9/30/2014)... 2014  RegeneRx Biopharmaceuticals, Inc. (OTC: RGRX) ("the ... received a Canadian Patent for Inhibition or Reversal ... includes claims using thymosin beta 4, various fragments ... these purposes.  The patent will expire in 2021. ... www.regenerx.com ) RegeneRx is focused ...
(Date:9/30/2014)... Sept. 30, 2014  KemPharm, Inc., a clinical-stage ... development of proprietary new molecular entity (NME) prodrugs, ... Trademark Office (USPTO) issued U.S. Patent No. 8,816,083 ... Acid, Benzoic Acid Derivatives and Heteroaryl Carboxylic Acid ... Use Thereof." The patent, which extends ...
(Date:9/30/2014)... (PRWEB) September 30, 2014 The custom ... ISPE Boston Product Show 2014. The Boston Area Chapter ... the 23rd annual, day-long event for Wed., Oct. 1 ... promises to be the largest in ISPE Boston’s long ... upward of 3,000 attendees expected. , HOLLOWAY AMERICA ...
(Date:9/30/2014)... September 30, 2014 UFP Technologies, ... specialty packaging has recently introduced a custom insulated ... Pack . The new insulated shipper solves the ... distribution process. UFP Technologies’ BioShell is a universal ... during storage, handling and shipping. The insulated shipper ...
Breaking Biology Technology:RegeneRx Receives Canadian Patent for Inhibition or Reversal of Skin Aging 2KemPharm, Inc. Receives Patent from the USPTO for Novel Prodrug of Hydromorphone, KP511 2HOLLOWAY AMERICA to Exhibit at ISPE Boston Product Show 2014 2HOLLOWAY AMERICA to Exhibit at ISPE Boston Product Show 2014 3UFP Technologies Introduces Insulated Shipping Container For Bulk Drug Transportation 2UFP Technologies Introduces Insulated Shipping Container For Bulk Drug Transportation 3
... of a three-part series focusing on the past, present and future of ... to WTNs interview with Nettles in March 2003. , , ... the next six to 12 months, what Dept. of Commerce initiatives would ... I had a meeting with Gov. Doyle a couple of weeks ...
... people who helped North Carolina and Los Angeles become ... of angel investors shared their most effective practices Tuesday ... ,Their techniques included educational programsbut you have to be ... the Council for Entrepreneurial Development , based in ...
... The second annual Wisconsin Entrepreneurs Conference got off to a ... treating at least a hundred entrepreneurs and industry representatives to ... The entrepreneurial boot camp was designed serve both veteran ... success from a panel of seasoned industrialists. , ,Our purpose ...
Cached Biology Technology:WTN exclusive interview with Commerce Secretary Cory Nettles (part 2) 2WTN exclusive interview with Commerce Secretary Cory Nettles (part 2) 3WTN exclusive interview with Commerce Secretary Cory Nettles (part 2) 4WTN exclusive interview with Commerce Secretary Cory Nettles (part 2) 5Experts share advice for Wisconsin entrepreneur programs 2Experts share advice for Wisconsin entrepreneur programs 3Experts share advice for Wisconsin entrepreneur programs 4Entrepreneurs get crash course for building a successful company 2Entrepreneurs get crash course for building a successful company 3
(Date:9/30/2014)... Health (NIH) awarded Lawrence Livermore National Laboratory (LLNL) ... system that will enable researchers to better understand ... scale. , LLNL,s grant-funded project is part of ... (Brain Research through Advancing Innovative Neurotechnologies) Initiative , ... of the human mind and uncover ways to ...
(Date:9/30/2014)... live in almost any aquatic environment on Earth, but ... species are pushed to the limit. The amount of ... critical for how different species cope in the future, ... of Gothenburg, published in the scientific journal Proceedings ... continues apace thanks to increasing levels of greenhouse gases ...
(Date:9/30/2014)... want your doctor to know what goes wrong with your ... idea to know what "normal" actually is. That,s where a ... the FASEB Journal comes in. In ... key set of molecules that can help scientists "see" which ... What,s more, they found never-before-detected gene activity and that men ...
Breaking Biology News(10 mins):NIH taps lab to develop sophisticated electrode array system to monitor brain act 2NIH taps lab to develop sophisticated electrode array system to monitor brain act 3Shape up quickly -- applies to fish, too! 2Scientists identify which genes are active in muscles of men and women 2
... researchers from Princeton University and the Drexel University College ... malaria breaks down an important amino acid in its ... By depleting this substance called arginine, the parasite may ... disease. The scientists believe that shedding light on ...
... and University of Malaya (UM) today announced the establishment ... This is the first Centre of its kind in ... communities in terms of their diet and disease, conducting ... environment and health, providing assistance for the national cancer ...
... of the oil in the ocean bubbles up naturally from ... was of no value. Likewise, NASA satellites collect thousands of ... of it gets passed over because no one thinks there ... black gold bubbling up from an otherwise undistinguished mass of ...
Cached Biology News:Malaria parasite zeroes in on molecule to enhance its survival, team finds 2Malaria parasite zeroes in on molecule to enhance its survival, team finds 3Queen's University Belfast improves Malaysian public health 2Scientists find black gold amidst overlooked data 2Scientists find black gold amidst overlooked data 3
Makes gels easy to load and track during electrophoresis. 10X concentrate of Bromophenol Blue, Xylene Cyanol, glycerol solution in TE...
Xanthine Oxidase (Bovine Buttermilk). Monospecific by IEP. Some cross reactivity with Xanthine Oxidase of other species and tissues may occur....
Rabbit polyclonal to XTP4 ( Abpromise for all tested applications). entrezGeneID: 84299 SwissProtID: Q9BRT3...
Low melting point. Ideally suited for in gel enzymatic reactions. Gelling temp. 24-28C Melting temp. 65C. EEO 0.12 Resolution 0.5-20 kb; Rnase/Dnase: none detected....
Biology Products: