Navigation Links
Better RNA Amplification

href="http://www.ambion.com/catalog/CatNum.php?1334">MEGAscript technology, requiring shorter IVT incubation times, thus enabling more reliable access to expression information from total RNA samples of less than 1 g. Both kits are effective with a wide dynamic range of RNA input (100 pg-5 g). A single round of amplification performed with MessageAmp II on samples as small as 100 ng results in up to 5000 fold amplification, generating sufficient aRNA for GeneChip analysis. These improvements in the MessageAmp II Kit also carry through to two-round reactions, facilitating robust amplification of total RNA from samples as small as 100 pg.

Figure 1. ArrayScript Reverse Transcriptase in MessageAmp II Produces Up to Twice the aRNA Yield. A comparison of ArrayScript RT (AS; 200 U), Competitor RT (Comp; 200 U); wild type M-MLV RT (200 U), and wild type AMV RT (10 U) using MessageAmp II conditions demonstrates that ArrayScript outperforms other commercial RTs. 90 ng HeLa-S3 total RNA (28S/18S rRNA ratio = 1.7) was heat denatured in the presence of T7 oligo(dT) at 70C for 10 min. Reverse transcription was initiated with the RT enzyme and incubated at 42C for 2 hr. Following first strand cDNA synthesis, DNA polymerase was added for second strand synthesis, which was incubated at 16C for 2 hr. The double-stranded cDNA was then filter purified and added to a 4 hr in vitro transcription reaction. The resulting aRNA was purified and analyzed by NanoDrop Spectrophotometer.


MessageAmp II vs. Affymetrix Protocol

We assessed the reproducibility and consistency of the MessageAmp II protocol compared to the protocol recommended by Affymetrix
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Better Way to Isolate RNA AND Protein From the Same Sample
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better RNA Amplification

(Date:8/29/2014)... MA (PRWEB) August 29, 2014 A ... in the biotech start-up dense realm of Boston-Cambridge, is ... and able investors. James Sherley, the Director of Boston’s ... increasing the local and national visibility of his company ... September 2013. , In addition to a social media ...
(Date:8/28/2014)... (PRWEB) August 28, 2014 Best Sanitizers, ... for the food processing industry, is asking industry professionals ... side comparison of the E2 soap they’re currently using ... Foam Soap . Hand hygiene is critical to ... food processing environment. Six key criteria are identified to ...
(Date:8/28/2014)... A new research platform uses a laser ... structures undergoing stress and heating, an approach ... for microelectronics and batteries., This new technique, ... how heating and the surface stress of ... have discussed the merits of surface-stress influence ...
(Date:8/28/2014)... Grove, PA (PRWEB) August 28, 2014 ... and create a culture that will allow individuals to ... are becoming increasingly important for employers looking to compete ... science industries are embracing this trend, as the sector ... fabric of a company, which will have real results ...
Breaking Biology Technology:The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 2The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 3The Adult Stem Cell Technology Center, LLC Participates in Multiple Stem Cell and Regenerative Medicine Conferences Before Year End 4Best Sanitizers, Inc. Asks Food Industry Professionals: With Fall Harvest On the Way, Is Your E2 Hand Soap Up to the Task? 2New analytical technology reveals 'nanomechanical' surface traits 2New analytical technology reveals 'nanomechanical' surface traits 3Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 2Klein Hersh International Leads Philadelphia Area Life Science Industry in Company Culture 3
... 2010 Advanced Life Sciences Holdings, Inc. ... company engaged in the discovery, development and commercialization of novel ... diseases, today announced that the Company has been awarded a ... created by Congress as part of the Patient Protection and ...
... 2010 Sangamo BioSciences, Inc. (Nasdaq: ... awarded a total of $978,000 in grants for four ... Program. Under the recently enacted Patient ... of the Internal Revenue Code, cash grants were awarded ...
... OF TWO NEW MEMBERS TO THE ADVISORY BOARD ... ... if (typeof(lpUnit)==,undefined,) var lpUnit=,PRN,; --> ... s = ,; var first_result; // Results are keyed by longUrl, so we need to grab the first one. ...
Cached Biology Technology:IRS Awards Cash Grant to Advanced Life Sciences Under the Qualifying Therapeutic Discovery Project 2IRS Awards Cash Grant to Advanced Life Sciences Under the Qualifying Therapeutic Discovery Project 3Sangamo BioSciences Awarded Grants for the Development of Four ZFP Therapeutic® Programs 2Sangamo BioSciences Awarded Grants for the Development of Four ZFP Therapeutic® Programs 3IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 2IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 3IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 4IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 5IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 6IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 7IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 8IGEN NETWORKS ANNOUNCES THE APPOINTMENT OF TWO NEW MEMBERS TO THE ADVISORY BOARD 9
(Date:8/28/2014)... peer groups and clinicians is critical to the ... University of Georgia research. , A qualitative study ... determined that role models for successful breastfeeding help ... , "Mothers who received that support are more ... co-author Alex Anderson, an associate professor in the ...
(Date:8/28/2014)... complex systems. Their infrastructure equipment requires ancillary sub-systems ... to supply fuel, and ICT systems for control ... is interconnected with a wider network and they ... scientists has studied various aspects of inter-network dependencies, ... in EPJ B by Gaihua Fu from Newcastle ...
(Date:8/28/2014)... could pose a significant health risk to people with asthma ... of Allergy and Clinical Immunology . , By critically reviewing ... research has found that the presence of several types of ... well as increasing the likelihood of developing the condition. , ... University of Exeter Medical School and is the first time ...
Breaking Biology News(10 mins):Breastfeeding study shows need for effective peer counseling programs 2Inter-dependent networks stress test 2Indoor mold poses health risk to asthma sufferers 2
... PORTLAND, Ore. -- Zeroing in on the early cell mutations that ... to find a personalized therapy to stop it. Scientists were able ... out the order in which these abnormalities form to analyze the ... The study,s findings, which are published in the July edition of ...
... -- The onset of puberty is a critical moment ... the stakes are particularly high in countries where the ... health abound. Yet to date, the healthcare community in ... instead concentrate efforts on young women who are older ...
... French . Montreal, June 29, 2011 What ... likely to become compulsive shoppers and men more likely to ... when they drive expensive sports cars? How does the fashion ... religions, cosmetic companies, and self-help gurus cater to consumers, Darwinian-based ...
Cached Biology News:Scientists develop method to determine order of mutations that lead to cancer 2Girl's Puberty Book Project provides an international model for promoting female health in Africa 2Girl's Puberty Book Project provides an international model for promoting female health in Africa 3Natural-born consumers 2
... ESGRO Complete Derivation Kit is ... ES cells from delayed blastocysts in ... Clonal ... Delayed Blastocyst Incubation Medium Trypsin ...
streptavidin, Alexa Fluor® 750–allophycocyanin conjugate (Alexa Fluor® 750–allophycocyanin streptavidin) *1 mg/mL*...
Template and primers for preparation of internal control spots...
Biotin-OSu is used to label proteins, immunological agents, DNA and synthetic oligonucleotides. Biotin labeling is detected through enzymatically labeled avidin or streptavidin proteins that conforma...
Biology Products: