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NEW Amino Allyl MessageAmp II Kit now with Cy Dyes included! *
Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis
Greater sensitivity due to higher yields of full-length cDNA
aRNA synthesis powered by MEGAscript, the best technology for in vitro transcription
High labeling efficiency yields consistently high percent present calls and low 3'/5' ratios in GeneChip analysis
The MessageAmp aRNA Amplification Kit was the first commercially available set of Eberwine-based aRNA amplification reagents, and the Amino Allyl MessageAmp aRNA Amplification Kit was the first kit to integrate amino allyl incorporation and aRNA amplification (see Incorporating Amino Allyl UTP During Amplification, at right). Now newer versions of both kits feature improvements that provide even greater yields of longer aRNA from inputs as small as 100 ng in a single round of amplification.
Improved RNA Amplification
The use of a modified M-MLV reverse transcriptase, ArrayScript,
in conjunction with the optimization of the second-strand cDNA synthesis
reaction results in increased conversion of mRNA into full-length, double-stranded
cDNA templates. ArrayScript produces the highest yields of full-length
cDNA compared to other reverse transcriptases (Figure 1). The second-strand
cDNA synthesis reaction has been optimized for maximal conversion of first-strand
cDNA into full-length double-stranded cDNA. The in vitro transcription
(IVT) reaction is powered by '"/>
Source:
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