Navigation Links
Better RNA Amplification

NEW Amino Allyl MessageAmp II Kit now with Cy Dyes included! *


Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis

Greater sensitivity due to higher yields of full-length cDNA

aRNA synthesis powered by MEGAscript, the best technology for in vitro transcription

High labeling efficiency yields consistently high percent present calls and low 3'/5' ratios in GeneChip analysis

The MessageAmp aRNA Amplification Kit was the first commercially available set of Eberwine-based aRNA amplification reagents, and the Amino Allyl MessageAmp aRNA Amplification Kit was the first kit to integrate amino allyl incorporation and aRNA amplification (see Incorporating Amino Allyl UTP During Amplification, at right). Now newer versions of both kits feature improvements that provide even greater yields of longer aRNA from inputs as small as 100 ng in a single round of amplification.


Improved RNA Amplification

The use of a modified M-MLV reverse transcriptase, ArrayScript, in conjunction with the optimization of the second-strand cDNA synthesis reaction results in increased conversion of mRNA into full-length, double-stranded cDNA templates. ArrayScript produces the highest yields of full-length cDNA compared to other reverse transcriptases (Figure 1). The second-strand cDNA synthesis reaction has been optimized for maximal conversion of first-strand cDNA into full-length double-stranded cDNA. The in vitro transcription (IVT) reaction is powered by '"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Better Way to Isolate RNA AND Protein From the Same Sample
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better RNA Amplification

(Date:12/20/2013)... Tampa, Fla. USA (PRWEB) December 20, 2013 ... by Janet Woodcock, M.D., Director of the US ... in her statement associated with her testimony to ... Reform’s Subcommittee on Energy Policy, Health Care and ... Check Up: Drug Development and Manufacturing Challenges,” Dr. ...
(Date:12/20/2013)... , 20. Dezember 2013 Wie ... Innovator bei der personalisierten Medizin, bekanntgab, hat ... im US-Bundesstaat Arizona von der American ... erste Akkreditierung als medizinisches Laboratorium nach ISO ... die Qualität und Kompetenz" im Bereich molekulares ...
(Date:12/20/2013)... DuPont congratulates David Hula of Charles City, ... Pioneer® brand hybrid P2088YHR in the 2013 National Corn ... Winning the No-Till/Strip-Till Irrigated category, David Hula produced an ... ever recorded by the NCGA in its annual contest. ... planting six different Pioneer® brand hybrids placed in the ...
(Date:12/20/2013)... ITRA Global announced today that Mylinda J. Vick, ... was re-elected chairman of the board of directors for 2014. ... Global remains one of the largest International organizations devoted ... thrilled to serve in the role of Chairman of the ... remains one of the few organizations that truly advocates for ...
Breaking Biology Technology:Two ISPE Studies Cited in FDA Statement to Congress 2Caris Life Sciences erhält erste Akkreditierung als medizinisches Laboratorium nach ISO 15189 im Bereich Tumor-Profiling 2Caris Life Sciences erhält erste Akkreditierung als medizinisches Laboratorium nach ISO 15189 im Bereich Tumor-Profiling 3Caris Life Sciences erhält erste Akkreditierung als medizinisches Laboratorium nach ISO 15189 im Bereich Tumor-Profiling 4Caris Life Sciences erhält erste Akkreditierung als medizinisches Laboratorium nach ISO 15189 im Bereich Tumor-Profiling 5DuPont Congratulates David Hula for Breaking Corn Yield World Record with Pioneer® Brand Hybrid P2088YHR in 2013 NCGA Corn Yield Contest 2DuPont Congratulates David Hula for Breaking Corn Yield World Record with Pioneer® Brand Hybrid P2088YHR in 2013 NCGA Corn Yield Contest 3ITRA Global Elects 2014 Board of Directors and Officers 2
... MyWeather LLC has named Kevin Baird as president ... went to Carleton College and began his career working ... He has served as vice resident of sales and ... vice president of sales and marketing for Achieve3000, a ...
... Researchers at the Ludwig Institute for Cancer ... , have developed a method to identify thousands of regulatory ... today in Nature. , ,The purpose of the study was ... as promoters, the starting points of the genes that determine ...
... - Governor Jim Doyle announced that the Department ... provides low-interest loans as seed capital to Wisconsin high-tech ... loan to Madison-based GenTel BioSurfaces, Inc. , helping ... about the size of a microscope slide that contain ...
Cached Biology Technology:NimbleGen identifies human genome sequences 2
(Date:12/20/2013)... scientists have assumed that if mercury is high and ... Arctic, the same is true of fish elsewhere in ... the U.S., Russia, and Canada has discovered that assumption ... In addition to differences in mercury processes as a ... turns out that the economic decline of the former ...
(Date:12/20/2013)... team of material scientists, chemical engineers and physicists from ... their effort to use liquid crystals as a medium ... team produced patterns of "defects," useful disruptions in the ... and rings. The new study adds a more complex ... array in the shape of a flower. ...
(Date:12/20/2013)... funded by the National Science Foundation (NSF) and led ... will attempt to characterize the complex dynamics of mercury ... ecosystems. , Mercury is a neurotoxic element found naturally ... fuel combustion, mining, smelting and waste combustion have increased ... to five over the last 150 years. That increased ...
Breaking Biology News(10 mins):Virginia Tech research overturns assumption about mercury in the Arctic 2Virginia Tech research overturns assumption about mercury in the Arctic 3Penn researchers grow liquid crystal 'flowers' that can be used as lenses 2Penn researchers grow liquid crystal 'flowers' that can be used as lenses 3New DRI project aimed at understanding Mercury dynamics in the Arctic tundra 2
... has published the genome sequence of the rhesus macaque ... genomes. Published April 13 in a special section of ... primate species share about 93 percent of their DNA, ... In its paper, the Rhesus Macaque Genome Sequence and ...
... people regain strength and normal use of affected hands ... California, Irvine study. , Stroke patients with ... release objects after therapy sessions using the Hand-Wrist Assisting ... residual weakness and reduced function of the right hand, ...
... arrival of genetically modified crops has added another level ... a sociocultural anthropologist at Washington University in St. Louis. ... of environmental studies, both in Arts & Sciences, at ... detailed anthropological fieldwork on these crops and the way ...
Cached Biology News:Analysis of rhesus monkey genome uncovers genetic differences with humans, chimps 2Analysis of rhesus monkey genome uncovers genetic differences with humans, chimps 3Robotic therapy helps restore hand use after stroke 2Robotic therapy helps restore hand use after stroke 3New technologies coming too fast for Indian farmers 2
Recognizes NSCL1. The molecular weight of the protein is 14,616 Daltons....
Secure-Seal™ spacer, one well, 20 mm diameter, 0.12 mm deep *set of 100*...
Rat Antibody to Mouse TCR VB8.1,8.2:FITC Recognises the V beta 8.1 and V beta 8.2 determinants of the murine T cell receptor. Specificity: mouse TCR VB8.1 and 8.2...
KAI 1 (G-2)...
Biology Products: