Navigation Links
Better RNA Amplification

NEW Amino Allyl MessageAmp II Kit now with Cy Dyes included! *


Amplify as little as 100 ng of input RNA in a single round for GeneChip analysis

Greater sensitivity due to higher yields of full-length cDNA

aRNA synthesis powered by MEGAscript, the best technology for in vitro transcription

High labeling efficiency yields consistently high percent present calls and low 3'/5' ratios in GeneChip analysis

The MessageAmp aRNA Amplification Kit was the first commercially available set of Eberwine-based aRNA amplification reagents, and the Amino Allyl MessageAmp aRNA Amplification Kit was the first kit to integrate amino allyl incorporation and aRNA amplification (see Incorporating Amino Allyl UTP During Amplification, at right). Now newer versions of both kits feature improvements that provide even greater yields of longer aRNA from inputs as small as 100 ng in a single round of amplification.


Improved RNA Amplification

The use of a modified M-MLV reverse transcriptase, ArrayScript, in conjunction with the optimization of the second-strand cDNA synthesis reaction results in increased conversion of mRNA into full-length, double-stranded cDNA templates. ArrayScript produces the highest yields of full-length cDNA compared to other reverse transcriptases (Figure 1). The second-strand cDNA synthesis reaction has been optimized for maximal conversion of first-strand cDNA into full-length double-stranded cDNA. The in vitro transcription (IVT) reaction is powered by '"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. A Better Way to Isolate RNA AND Protein From the Same Sample
2. Designing a Better siRNA
3. RNA Amplification Just Got Better: MessageAmp II
4. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
5. Simple Purification of DNA from Plasmid Minipreps, PCR Amplifications and Agarose Gels
6. Greater Amplification Specificity with New Hot Start PCR Enzyme
7. Enhanced Amplification of Long Targets with PfuTurbo DNA Polymerase
8. Optimizing pfuturbo DNA Polymerase Amplification Reactions with Perfect Match PCR Enhancer
9. Improve Amplification Specificity with Hot Start PCR Enzyme
10. Amplification of the amoA Gene of Nitrosococcus oceani Bacteria Using Eppendorf MasterTaq
11. Amplification of Mouse cDNAs for Microarrays Using the Eppendorf MasterTaq Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Better RNA Amplification

(Date:8/22/2014)... (August 22nd), in the scientific journal PLoS ... establish the effect of serotonin on sensitivity to ... optical techniques., "Serotonin is a small molecule known ... brain functions, from the control of sleep and ... This neurotransmitter is also popularly thought to contribute ...
(Date:8/22/2014)... , Aug. 22, 2014 Research ... "Global and China Gel Permeation Chromatography (GPC) Industry ... The Global and China Gel Permeation Chromatography (GPC) ... the current state of the global gel permeation chromatography ... The report provides a basic overview of the industry ...
(Date:8/22/2014)... SC (PRWEB) August 22, 2014 ... indisputable acknowledgement is a national disgrace. Furthermore, every ... in establishing the current, deplorable state of unorganized ... "5 great mistakes," to merely serve as a ... the extent of colossal failure(s) that have accumulated ...
(Date:8/21/2014)... 2014 2014 Deep Research ... a professional and in-depth research report on ... basic Seaweed Fertilizer information, including Seaweed Fertilizer ... as well as industry overview. This research ... market as well as global industry analysis ...
Breaking Biology Technology:From happiness to pain: Understanding serotonin's function 2Global and China Gel Permeation Chromatography (GPC) Industry Report 2014 25 Great Mistakes in Healthcare and How to Fix Them by Dr. Ira Williams 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 2Internationals Seaweed Fertilizer Industry 2014 Development Trend Analysis in New Research Study at DeepResearchReports.com 3
... 15 Senetek PLC,(OTC Bulletin Board: SNKTY), a Life ... the science of healthy aging, today,announced an agreement with ... Sciences in Poznan, Poland for the exclusive rights to ... therapeutic technology for,the potential use against a broad range ...
... 15 bwin, the world,s,leading online gaming company, and ... Harvard Medical School Teaching Affiliate, have,just signed an agreement ... to use scientific evidence to create the safest,possible online ... longitudinal study. , ...
... ProGenTech Limited was a winner of the Red Herring 100 Award, ... Asia. , The Red Herring ... top 100 out of more than 1,000,closely evaluated companies that are ... "It is truly an honor to be recognized by ...
Cached Biology Technology:Senetek PLC Acquires Rights to Additional Anticancer RNAi Technology 2bwin and Harvard Medical School Faculty Sign 5-Year Research Cooperation Agreement 2Red Herring Awards ProGenTech Limited for the 2008 Red Herring Asia 100 2
(Date:8/22/2014)... an injury can probably remember the after-effects, including pain, ... is fighting back against the injury. When tissue in ... aid in tissue regeneration. An inflammatory response acts as ... the body to heal after injuries such as wounds ... healing in situations in which foreign material is introduced, ...
(Date:8/22/2014)... comprehensive study of captive black rhino reproduction in ... success of breeding programmes. , Researchers from Chester ... of Liverpool carried out a six-year study which ... , Dr Katie Edwards led the research as ... Liverpool. She says: "Although some black rhinoceros breed ...
(Date:8/22/2014)... will finally be able to purchase fuel cell cars ... vehicles, most of the cars will run on hydrogen ... to global warming. , Now scientists at Stanford University ... ordinary AAA battery to produce hydrogen by water electrolysis. ... electrodes that split liquid water into hydrogen and oxygen ...
Breaking Biology News(10 mins):Tissue regeneration using anti-inflammatory nanomolecules 2Hormone analysis helps identify horny rhinos 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 2Stanford scientists develop a water splitter that runs on an ordinary AAA battery 3
... ultra-low input power densities, researchers at the University of ... how low-power "optical nanotweezers" can be used to trap, ... "We already know that plasmonic nanoantennas enhance ... and thus, previously showed that we can use these ...
... John Trumble , a distinguished professor of entomology ... Integrated Pest Management Team Award from the Entomological ... people about science through insects. Sponsored by Dow AgroSciences, ... work team approach to pest control." Trumble and the ...
... WINSTON-SALEM, N.C. The Maya Angelou Center for Women,s ... it will hold a three-day international summit focusing on ... September 27 through Saturday, September 29. The Maya Angelou ... and will bring together internationally recognized advocates for women,s ...
Cached Biology News:Newly demonstrated capabilities of low-powered nanotweezers may benefit cellular-level studies 2Team including UC Riverside entomologist honored for research leading to healthier potato chips 2Maya Angelou Center Brings International Women’s Health Summit to Winston-Salem, NC 2Maya Angelou Center Brings International Women’s Health Summit to Winston-Salem, NC 3
... Adapted to Sf-900 ... were isolated from Spodoptera ... insect cells (1). The ... to serum-free suspension culture ...
... BAC (100+kb), fosmid (40kb), and large plasmid ... any othe BAC vector. On command 20-50 ... easy recombinant DNA purification. Available with blue-white ... vectors for the highest insert stability. Kits ...
MAGP-2 (C-19)...
... Immunogen: Synthetic peptide derived from ... junctophilin-2. Specificity: Specific for the ... controls: mouse skeletal muscle homogenates frozen ... Western blotting Immunohistochemistry(froz) ImmunofluorescenceStorage: Store at ...
Biology Products: