Navigation Links
Benchmark Plus Microplate Reader Scan Well Feature

istencies in absorbance reading.

4. Incubate 5 min at room temperature.

5. Place microplate into Benchmark Plus microplate reader.

6. Open Microplate Manager. Under File, select Endpoint protocol.

7. Select 96-well format. Under Advanced Options, open Scan Well.

8. Scan well A1 at 340800 nm using a 5 nm step size. Save data.

9. Scan remaining wells (A2A7) and save data.

10. For bovine IgG at optimal wavelength, select Endpoint protocol, 96-well format, and single wavelength reading at 590 nm.

11. Highlight the appropriate wells to be read; i.e., A1 to C7 for the concentration range from 62.5 g/ml to 1 mg/ml and the blank. 12. Read absorbance. Save and analyze data.


Results
The most concentrated protein sample shows a higher peak at 590 nm than the least concentrated protein samples (Figure 1). The least concentrated samples and the blank showed higher peaks at 470 nm, whereas the more concentrated protein samples showed similar peak heights at around 470 and 590 nm. From the figure, one can conclude that whereas a peak at 470 nm is apparently caused by the cell culture medium used here, the optimal wavelength for bovine IgG is at 590 nm.

Further reading of bovine IgG concentration at 590 nm on Benchmark Plus yields a linear graph with a correlation coefficient of 0.9993 (Figure 2).


Conclusion
The Benchmark Plus Scan Well feature is used to ascertain the best wavelength for reading a desired assay. It allows scanning of a well from 340800 nm at various wavelength increments. A graph and data of the scan area are provided and can be exported into Microsoft Excel software for furt
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Cell Proliferation Assay Using the Benchmark Plus Microplate Spectrophotometer
2. Benchmark Plus Microplate Reader: Quantitation of Protein Concentration Using Two Different Colorimetric Assay Kits, Rev A
3. Cerenkov Counting Performance on the TopCountTM Microplate Scintillation and Luminescence Counter
4. Counting Non-Aqueous Samples in the TopCount Microplate Scintillation and Luminescence Counter
5. Fluorometric Protease Assays in the SpectraMax Gemini Microplate Spectrofluorometer: Example Using Caspase3 (MaxLine Application Note #35)
6. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
7. Using the OligoGreen Oligonucleotide Quantitation Reagent in the Gemini XS, Gemini EM and SpectraMax M2 Microplate Readers (MaxLine Application Note #21)
8. PathCheck Applied to Measurement of Protein Solutions in the SpectraMax Plus Microplate Spectrophotometer (MaxLine Application Note #26)
9. Microdetermination of Phosphorus Using the SpectraMax Plus Microplate Spectrophotometer: Choice of Microplate, Cuvette, or Test Tube Assay Formats (MaxLine Application Note #24)
10. UV Absorbance Measurements in SpectraMax Microplate Spectrophotometers MaxLine Application Note #32)
11. Ca++-Induced G Protein Coupled Receptor (GPCR) Activation Monitored via Aequorin Luminescence in the LMax Microplate Luminometer (MaxLine Application Note #42)
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Benchmark Plus Microplate Reader Scan Well Feature

(Date:1/14/2014)... Toronto, Canada (PRWEB) January 14, 2014 ... and its motives and methods in product development and ... of the industry. This mistrust, fueled by concerns about ... further fed by reports of spectacular fines to the ...
(Date:1/14/2014)... Kinaxis ®, provider of RapidResponse ®, a cloud-based supply ... ( S&OP ) service, is proud to be a gold ... held at the Hilton San Diego Bayfront, January 30 – ... Kaszas , Director of Supply Chain at Amgen, as she ...
(Date:1/14/2014)... During the 1600’s through the 1800’s doctors contributed unknowingly to ... time period, doctors did not know that they needed to ... of vulnerable patients. In the same way, medical interns and ... transmitting herpes viruses to their patients. The CBCD explains. , ...
(Date:1/14/2014)... Springfield, MO (PRWEB) January 14, 2014 ... a comprehensive database of material test reports (MTRs) for ... and fabricates. Documenting test results is particularly important for ... other regulations. The MTR Library features search functionality that ...
Breaking Biology Technology:The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 2The Sunshine Act: Necessary Regulation or Unnecessary Dysregulation? New Life Science Webinar Hosted by Xtalks and IRB Services 3Event Alert: Kinaxis Customer to Present at the Biomanufacturing Summit "Supply Chain: Improving Network Effectiveness" 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 2Study: Fatigued Medical Interns Infect Their Patients with Herpes Viruses; The CBCD Sees a Parallel with “The Doctor’s Plague” 3Pressure Vessel Design and Fabrication Company Builds Database for Material Test Reports 2Pressure Vessel Design and Fabrication Company Builds Database for Material Test Reports 3
... JOLLA, Calif., Nov. 22, 2011  If algae is to be ... workers are needed to make that a reality. Applications are ... California San Diego to retrain workers as general science technicians ... and Imperial County region. Approximately 55 students ...
... Sequenom, Inc. (NASDAQ: SQNM ), a life ... Company,s participation at the 23rd Annual Piper Jaffray Healthcare Conference ... 2011. Ronald M. Lindsay, Ph.D., Director and ... will present on Tuesday, November 29, 2011 beginning at 8:30 ...
... Among the many hurdles to be cleared before human embryonic ... or not transplanted cells can functionally integrate into target organs ... Proceedings of the National Academy of Sciences, a ... lab from blank slate human embryonic stem cells and implanted ...
Cached Biology Technology:Free Job Training: 55 Grants Available to Retrain Workers for Biofuels Industry 2Free Job Training: 55 Grants Available to Retrain Workers for Biofuels Industry 3Sequenom Announces Participation at the 23rd Annual Piper Jaffray Healthcare Conference 2Implanted neurons, grown in the lab, take charge of brain circuitry 2Implanted neurons, grown in the lab, take charge of brain circuitry 3
(Date:4/17/2014)... trials to treat tuberculosis could be the basis for ... bacteria, fungal infections and parasites, yet evade resistance, according ... collaborators. , Led by U. of I. chemistry professor ... drug SQ109 attacks the tuberculosis bacterium, how the drug ... to malaria and how targeting multiple pathways reduces ...
(Date:4/17/2014)... modeling study suggests that fish consumption advisories for ... to long-lived contaminants like persistent organic pollutants (POPs). ... of researchers including University of Toronto Scarborough PhD ... at how different levels of environmental contamination, a ... chemicals in the body influenced exposure in her ...
(Date:4/17/2014)... University engineer has developed a patented technique that ... devices. The same technique could help police during ... and Kay L. Theede chair in engineering and ... his research team have created a template-based system ... trunks. The distance detection method called stand-off ...
Breaking Biology News(10 mins):Multitarget TB drug could treat other diseases, evade resistance 2Fish consumption advisories fail to cover all types of contaminants 2Patented research remotely detects nitrogen-rich explosives 2
... Sharing is a behavior on which day care workers and kindergarten ... for our ape cousins the bonobos, sharing just comes naturally. ... Current Biology, it looks like bonobos never seem to ... notorious for hogging food to themselves, by physical aggression if necessary. ...
... Imagine that you are emerging from the subway and heading ... in the wrong direction. For a moment, you feel disoriented, ... surrounding streets quickly helps you pinpoint your location, and you ... Research tells us that human adults, toddlers, rats, chicks ...
... the first to design a bioactive nanomaterial that promotes the ... of expensive growth factors. Minimally invasive, the therapy activates the ... therapy can do this. The results will be published ... of the National Academy of Sciences (PNAS). "Unlike ...
Cached Biology News:'Peter Pan' apes never seem to learn selfishness 2'Peter Pan' apes never seem to learn selfishness 3Ability to navigate may be linked to genes, researcher says 2Ability to navigate may be linked to genes, researcher says 3Growing cartilage -- no easy task 2
Hybridization Buffer 3.6 ml...
Chicken polyclonal to Thaumatin 2 ( Abpromise for all tested applications). Antigen: Full length Thaumatin 2...
Proteinase K Buffer 3.9 ml...
Rabbit polyclonal to VEGF Receptor 2 (phospho Y1214) ( Abpromise for all tested applications). entrezGeneID: 3791 SwissProtID: P35968...
Biology Products: